Strain Fitness in Escherichia coli BL21 around ECD_00298

Experiment: Bas51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00297 and ECD_00298 are separated by 51 nucleotidesECD_00298 and ECD_00299 are separated by 122 nucleotides ECD_00297: ECD_00297 - lactose permease, at 331,461 to 332,714 _00297 ECD_00298: ECD_00298 - beta-D-galactosidase, at 332,766 to 335,840 _00298 ECD_00299: ECD_00299 - lactose-inducible lac operon transcriptional repressor, at 335,963 to 337,045 _00299 Position (kb) 332 333 334 335 336Strain fitness (log2 ratio) -2 -1 0 1 2 3at 331.808 kb on + strand, within ECD_00297at 331.808 kb on + strand, within ECD_00297at 331.821 kb on - strand, within ECD_00297at 331.824 kb on - strand, within ECD_00297at 331.902 kb on - strand, within ECD_00297at 331.974 kb on - strand, within ECD_00297at 332.008 kb on + strand, within ECD_00297at 332.009 kb on - strand, within ECD_00297at 332.009 kb on - strand, within ECD_00297at 332.220 kb on - strand, within ECD_00297at 332.381 kb on + strand, within ECD_00297at 332.381 kb on + strand, within ECD_00297at 332.710 kb on + strandat 333.052 kb on + strandat 333.061 kb on + strandat 333.061 kb on + strandat 333.061 kb on + strandat 333.061 kb on + strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.062 kb on - strandat 333.138 kb on + strand, within ECD_00298at 333.288 kb on - strand, within ECD_00298at 333.291 kb on + strand, within ECD_00298at 333.353 kb on + strand, within ECD_00298at 333.354 kb on - strand, within ECD_00298at 333.428 kb on + strand, within ECD_00298at 333.454 kb on - strand, within ECD_00298at 333.738 kb on + strand, within ECD_00298at 333.745 kb on - strand, within ECD_00298at 333.809 kb on + strand, within ECD_00298at 333.858 kb on - strand, within ECD_00298at 334.105 kb on + strand, within ECD_00298at 334.106 kb on - strand, within ECD_00298at 334.331 kb on - strand, within ECD_00298at 334.347 kb on - strand, within ECD_00298at 334.381 kb on + strand, within ECD_00298at 334.615 kb on + strand, within ECD_00298at 334.615 kb on + strand, within ECD_00298at 334.615 kb on + strand, within ECD_00298at 334.615 kb on + strand, within ECD_00298at 334.615 kb on + strand, within ECD_00298at 334.616 kb on - strand, within ECD_00298at 335.077 kb on - strand, within ECD_00298at 335.190 kb on - strand, within ECD_00298at 335.840 kb on + strandat 335.890 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas51
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331,808 + ECD_00297 0.28 -1.4
331,808 + ECD_00297 0.28 +0.6
331,821 - ECD_00297 0.29 -0.1
331,824 - ECD_00297 0.29 +0.9
331,902 - ECD_00297 0.35 +0.3
331,974 - ECD_00297 0.41 -1.3
332,008 + ECD_00297 0.44 +1.9
332,009 - ECD_00297 0.44 -0.3
332,009 - ECD_00297 0.44 -0.5
332,220 - ECD_00297 0.61 -0.5
332,381 + ECD_00297 0.73 -1.4
332,381 + ECD_00297 0.73 -0.8
332,710 + -1.9
333,052 + -0.4
333,061 + +0.8
333,061 + -0.7
333,061 + -1.1
333,061 + +0.3
333,062 - +2.9
333,062 - -1.4
333,062 - +0.2
333,062 - +0.0
333,062 - +0.6
333,062 - +1.5
333,062 - -0.8
333,062 - +0.0
333,138 + ECD_00298 0.12 -1.0
333,288 - ECD_00298 0.17 -0.6
333,291 + ECD_00298 0.17 +0.9
333,353 + ECD_00298 0.19 +0.1
333,354 - ECD_00298 0.19 +0.2
333,428 + ECD_00298 0.22 +0.2
333,454 - ECD_00298 0.22 +0.1
333,738 + ECD_00298 0.32 -0.4
333,745 - ECD_00298 0.32 -1.4
333,809 + ECD_00298 0.34 -0.5
333,858 - ECD_00298 0.36 -1.7
334,105 + ECD_00298 0.44 -0.4
334,106 - ECD_00298 0.44 -1.4
334,331 - ECD_00298 0.51 -0.6
334,347 - ECD_00298 0.51 +0.1
334,381 + ECD_00298 0.53 -0.7
334,615 + ECD_00298 0.60 +1.1
334,615 + ECD_00298 0.60 +0.6
334,615 + ECD_00298 0.60 -1.4
334,615 + ECD_00298 0.60 +0.0
334,615 + ECD_00298 0.60 +0.2
334,616 - ECD_00298 0.60 -0.1
335,077 - ECD_00298 0.75 +0.4
335,190 - ECD_00298 0.79 -0.3
335,840 + +1.3
335,890 - -0.6

Or see this region's nucleotide sequence