Strain Fitness in Escherichia coli BW25113 around b4120
Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | inner cut, LB soft agar motility assay |
---|---|---|---|---|---|
remove | |||||
4,340,413 | - | melA | b4119 | 0.35 | +4.1 |
4,340,413 | - | melA | b4119 | 0.35 | +2.8 |
4,340,430 | + | melA | b4119 | 0.37 | -0.4 |
4,340,440 | + | melA | b4119 | 0.37 | +0.3 |
4,340,452 | - | melA | b4119 | 0.38 | -0.4 |
4,340,452 | - | melA | b4119 | 0.38 | -0.2 |
4,340,457 | - | melA | b4119 | 0.39 | -0.6 |
4,340,457 | - | melA | b4119 | 0.39 | +1.8 |
4,340,746 | + | melA | b4119 | 0.60 | -3.0 |
4,340,746 | + | melA | b4119 | 0.60 | +2.2 |
4,340,746 | + | melA | b4119 | 0.60 | +0.6 |
4,340,754 | - | melA | b4119 | 0.60 | +2.5 |
4,340,765 | + | melA | b4119 | 0.61 | +0.2 |
4,340,765 | + | melA | b4119 | 0.61 | +0.4 |
4,340,799 | - | melA | b4119 | 0.64 | -0.1 |
4,340,869 | + | melA | b4119 | 0.69 | -1.2 |
4,340,917 | - | melA | b4119 | 0.72 | -0.4 |
4,340,924 | + | melA | b4119 | 0.73 | +3.0 |
4,340,934 | - | melA | b4119 | 0.74 | -0.4 |
4,340,944 | - | melA | b4119 | 0.74 | +1.5 |
4,340,944 | - | melA | b4119 | 0.74 | +1.8 |
4,340,981 | - | melA | b4119 | 0.77 | +0.0 |
4,341,084 | - | melA | b4119 | 0.85 | +0.1 |
4,341,084 | - | melA | b4119 | 0.85 | -2.3 |
4,341,111 | - | melA | b4119 | 0.87 | +0.9 |
4,341,133 | - | melA | b4119 | 0.88 | +0.7 |
4,341,176 | + | -0.3 | |||
4,341,176 | - | -0.8 | |||
4,341,211 | - | +0.4 | |||
4,341,211 | - | -3.3 | |||
4,341,230 | + | -0.0 | |||
4,341,262 | - | -0.8 | |||
4,341,293 | - | +0.6 | |||
4,341,293 | - | -0.9 | |||
4,341,296 | + | -0.2 | |||
4,341,329 | + | +0.8 | |||
4,341,380 | + | +1.9 | |||
4,341,396 | - | -1.3 | |||
4,341,464 | + | +1.9 | |||
4,341,472 | - | -0.6 | |||
4,341,477 | - | -2.0 | |||
4,341,479 | + | -0.6 | |||
4,341,484 | + | +1.0 | |||
4,341,508 | + | +1.0 | |||
4,341,508 | + | +0.5 | |||
4,341,518 | - | -0.6 | |||
4,341,518 | - | -3.2 | |||
4,341,528 | + | +0.9 | |||
4,341,538 | + | melB | b4120 | 0.10 | -0.0 |
4,341,538 | + | melB | b4120 | 0.10 | -0.7 |
4,341,641 | + | melB | b4120 | 0.18 | +0.3 |
4,341,670 | + | melB | b4120 | 0.20 | +2.5 |
4,341,688 | + | melB | b4120 | 0.21 | +0.4 |
4,341,688 | + | melB | b4120 | 0.21 | +0.1 |
4,341,696 | + | melB | b4120 | 0.21 | -2.5 |
4,341,696 | + | melB | b4120 | 0.21 | +0.2 |
4,341,704 | - | melB | b4120 | 0.22 | -0.0 |
4,341,704 | - | melB | b4120 | 0.22 | -2.7 |
4,341,724 | + | melB | b4120 | 0.23 | -0.0 |
4,341,763 | - | melB | b4120 | 0.26 | -0.4 |
4,341,786 | + | melB | b4120 | 0.28 | -0.7 |
4,341,809 | - | melB | b4120 | 0.29 | -0.0 |
4,341,939 | + | melB | b4120 | 0.38 | +0.8 |
4,341,939 | + | melB | b4120 | 0.38 | -0.7 |
4,341,939 | + | melB | b4120 | 0.38 | -0.3 |
4,341,939 | + | melB | b4120 | 0.38 | +1.1 |
4,341,951 | - | melB | b4120 | 0.39 | +1.1 |
4,341,951 | - | melB | b4120 | 0.39 | +0.0 |
4,341,975 | + | melB | b4120 | 0.41 | -0.7 |
4,341,975 | + | melB | b4120 | 0.41 | -0.6 |
4,341,979 | - | melB | b4120 | 0.41 | -0.1 |
4,341,980 | + | melB | b4120 | 0.41 | -0.7 |
4,341,981 | - | melB | b4120 | 0.41 | +1.1 |
4,341,983 | - | melB | b4120 | 0.42 | -3.5 |
4,341,985 | + | melB | b4120 | 0.42 | +0.7 |
4,341,985 | + | melB | b4120 | 0.42 | -3.5 |
4,341,990 | + | melB | b4120 | 0.42 | +2.5 |
4,341,990 | + | melB | b4120 | 0.42 | -0.5 |
4,342,014 | + | melB | b4120 | 0.44 | +0.7 |
4,342,028 | + | melB | b4120 | 0.45 | +0.2 |
4,342,039 | - | melB | b4120 | 0.45 | +0.3 |
4,342,041 | + | melB | b4120 | 0.46 | -1.4 |
4,342,041 | + | melB | b4120 | 0.46 | -0.1 |
4,342,055 | - | melB | b4120 | 0.47 | -0.6 |
4,342,055 | - | melB | b4120 | 0.47 | -0.8 |
4,342,104 | - | melB | b4120 | 0.50 | -1.5 |
4,342,179 | - | melB | b4120 | 0.55 | +0.5 |
4,342,187 | + | melB | b4120 | 0.56 | -0.8 |
4,342,200 | + | melB | b4120 | 0.57 | +2.9 |
4,342,200 | + | melB | b4120 | 0.57 | -0.7 |
4,342,202 | + | melB | b4120 | 0.57 | +0.2 |
4,342,202 | + | melB | b4120 | 0.57 | +2.4 |
4,342,215 | - | melB | b4120 | 0.58 | +0.6 |
4,342,218 | - | melB | b4120 | 0.58 | -0.6 |
4,342,220 | + | melB | b4120 | 0.58 | +2.1 |
4,342,228 | - | melB | b4120 | 0.59 | -0.7 |
4,342,228 | - | melB | b4120 | 0.59 | -1.9 |
4,342,244 | - | melB | b4120 | 0.60 | -1.3 |
4,342,261 | - | melB | b4120 | 0.61 | -2.1 |
4,342,265 | - | melB | b4120 | 0.61 | -0.6 |
4,342,283 | + | melB | b4120 | 0.63 | +0.1 |
4,342,313 | - | melB | b4120 | 0.65 | -0.5 |
4,342,313 | - | melB | b4120 | 0.65 | -1.3 |
4,342,318 | - | melB | b4120 | 0.65 | -0.3 |
4,342,331 | - | melB | b4120 | 0.66 | +0.9 |
4,342,331 | - | melB | b4120 | 0.66 | +0.3 |
4,342,333 | - | melB | b4120 | 0.66 | -0.6 |
4,342,333 | - | melB | b4120 | 0.66 | -0.2 |
4,342,357 | - | melB | b4120 | 0.68 | +0.1 |
4,342,357 | - | melB | b4120 | 0.68 | +0.3 |
4,342,359 | - | melB | b4120 | 0.68 | -0.0 |
4,342,359 | - | melB | b4120 | 0.68 | -0.2 |
4,342,369 | - | melB | b4120 | 0.69 | -1.1 |
4,342,416 | + | melB | b4120 | 0.72 | -1.5 |
4,342,416 | + | melB | b4120 | 0.72 | +2.3 |
4,342,469 | - | melB | b4120 | 0.76 | +1.3 |
4,342,469 | - | melB | b4120 | 0.76 | -1.4 |
4,342,488 | - | melB | b4120 | 0.77 | -1.0 |
4,342,492 | + | melB | b4120 | 0.77 | -0.4 |
4,342,492 | + | melB | b4120 | 0.77 | +1.3 |
4,342,492 | + | melB | b4120 | 0.77 | +0.4 |
4,342,500 | - | melB | b4120 | 0.78 | -1.8 |
4,342,500 | - | melB | b4120 | 0.78 | +2.5 |
4,342,507 | + | melB | b4120 | 0.78 | -2.5 |
4,342,509 | + | melB | b4120 | 0.79 | +1.4 |
4,342,509 | + | melB | b4120 | 0.79 | +0.2 |
4,342,510 | - | melB | b4120 | 0.79 | +0.2 |
4,342,517 | - | melB | b4120 | 0.79 | -0.5 |
4,342,517 | - | melB | b4120 | 0.79 | +0.3 |
4,342,549 | + | melB | b4120 | 0.81 | +1.9 |
4,342,579 | - | melB | b4120 | 0.83 | +1.3 |
4,342,579 | - | melB | b4120 | 0.83 | +0.1 |
4,342,587 | + | melB | b4120 | 0.84 | +1.2 |
4,342,597 | + | melB | b4120 | 0.85 | -2.9 |
4,342,597 | + | melB | b4120 | 0.85 | +1.7 |
4,342,597 | + | melB | b4120 | 0.85 | -0.9 |
4,342,597 | + | melB | b4120 | 0.85 | +0.8 |
4,342,597 | + | melB | b4120 | 0.85 | -1.8 |
4,342,597 | + | melB | b4120 | 0.85 | +0.8 |
4,342,597 | + | melB | b4120 | 0.85 | +1.9 |
4,342,597 | + | melB | b4120 | 0.85 | -1.5 |
4,342,597 | + | melB | b4120 | 0.85 | -0.0 |
4,342,605 | - | melB | b4120 | 0.85 | -0.5 |
4,342,605 | - | melB | b4120 | 0.85 | -1.2 |
4,342,605 | - | melB | b4120 | 0.85 | -1.2 |
4,342,605 | - | melB | b4120 | 0.85 | +1.0 |
4,342,605 | - | melB | b4120 | 0.85 | +1.7 |
4,342,605 | - | melB | b4120 | 0.85 | +0.4 |
4,342,605 | - | melB | b4120 | 0.85 | +1.3 |
4,342,605 | - | melB | b4120 | 0.85 | -3.3 |
4,342,605 | - | melB | b4120 | 0.85 | -3.6 |
4,342,624 | + | melB | b4120 | 0.87 | -0.3 |
4,342,633 | - | melB | b4120 | 0.87 | -1.3 |
4,342,633 | - | melB | b4120 | 0.87 | +0.7 |
4,342,659 | + | melB | b4120 | 0.89 | -2.8 |
4,342,662 | - | melB | b4120 | 0.89 | -0.6 |
4,342,705 | - | -0.1 | |||
4,342,718 | + | +0.8 | |||
4,342,719 | - | +0.3 | |||
4,342,719 | - | +1.7 | |||
4,342,720 | - | -0.3 | |||
4,342,773 | + | +0.5 | |||
4,342,786 | - | +2.8 | |||
4,342,786 | - | +0.6 | |||
4,342,788 | + | +2.0 | |||
4,342,797 | - | -1.7 | |||
4,342,817 | + | +0.9 | |||
4,342,831 | - | +0.8 | |||
4,342,831 | - | +0.2 | |||
4,342,913 | + | +0.1 | |||
4,342,913 | + | -0.5 | |||
4,342,937 | + | +1.5 | |||
4,342,966 | + | -0.1 | |||
4,343,050 | - | yjdF | b4121 | 0.16 | -1.0 |
4,343,151 | + | yjdF | b4121 | 0.32 | +0.6 |
4,343,169 | - | yjdF | b4121 | 0.34 | +4.4 |
4,343,182 | - | yjdF | b4121 | 0.37 | +0.6 |
4,343,182 | - | yjdF | b4121 | 0.37 | -0.9 |
4,343,242 | + | yjdF | b4121 | 0.46 | -1.2 |
4,343,268 | - | yjdF | b4121 | 0.50 | +3.1 |
4,343,289 | + | yjdF | b4121 | 0.53 | -0.8 |
4,343,292 | + | yjdF | b4121 | 0.54 | +0.4 |
4,343,292 | + | yjdF | b4121 | 0.54 | +1.9 |
4,343,299 | + | yjdF | b4121 | 0.55 | -0.6 |
4,343,313 | - | yjdF | b4121 | 0.57 | -0.4 |
4,343,313 | - | yjdF | b4121 | 0.57 | +0.3 |
4,343,376 | - | yjdF | b4121 | 0.67 | -0.8 |
4,343,399 | + | yjdF | b4121 | 0.71 | +1.0 |
4,343,402 | - | yjdF | b4121 | 0.71 | -2.1 |
4,343,516 | + | yjdF | b4121 | 0.90 | -2.6 |
4,343,516 | + | yjdF | b4121 | 0.90 | +1.5 |
4,343,527 | + | +1.3 | |||
4,343,546 | + | +1.7 | |||
4,343,547 | + | -0.3 | |||
4,343,547 | + | +0.0 | |||
4,343,579 | + | +0.7 | |||
4,343,579 | + | -3.8 | |||
4,343,666 | + | +1.7 | |||
4,343,684 | - | -2.2 | |||
4,343,700 | + | -2.8 | |||
4,343,715 | + | +3.1 |
Or see this region's nucleotide sequence