Strain Fitness in Escherichia coli BW25113 around b2970
Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | inner cut, LB soft agar motility assay |
---|---|---|---|---|---|
remove | |||||
3,109,123 | - | -2.4 | |||
3,109,174 | + | -0.4 | |||
3,109,220 | - | +0.6 | |||
3,109,437 | + | yghE | b2969 | 0.30 | +1.3 |
3,109,538 | + | yghE | b2969 | 0.40 | -1.3 |
3,109,551 | + | yghE | b2969 | 0.42 | -0.9 |
3,109,704 | + | yghE | b2969 | 0.58 | -2.8 |
3,109,704 | + | yghE | b2969 | 0.58 | +0.7 |
3,109,712 | - | yghE | b2969 | 0.58 | -0.8 |
3,109,886 | - | yghE | b2969 | 0.76 | +1.2 |
3,109,933 | - | yghE | b2969 | 0.81 | +0.3 |
3,110,044 | - | -1.0 | |||
3,110,073 | + | +2.1 | |||
3,110,073 | + | -3.1 | |||
3,110,085 | - | +0.4 | |||
3,110,085 | - | -0.3 | |||
3,110,184 | + | +2.4 | |||
3,110,209 | - | yghF | b2970 | 0.11 | +0.5 |
3,110,231 | - | yghF | b2970 | 0.14 | -0.1 |
3,110,296 | - | yghF | b2970 | 0.21 | +3.5 |
3,110,328 | + | yghF | b2970 | 0.25 | +1.4 |
3,110,328 | + | yghF | b2970 | 0.25 | +3.7 |
3,110,329 | - | yghF | b2970 | 0.25 | +2.0 |
3,110,342 | - | yghF | b2970 | 0.27 | +1.4 |
3,110,426 | + | yghF | b2970 | 0.37 | +0.6 |
3,110,427 | + | yghF | b2970 | 0.37 | -0.0 |
3,110,427 | + | yghF | b2970 | 0.37 | -3.2 |
3,110,507 | + | yghF | b2970 | 0.46 | +0.1 |
3,110,550 | - | yghF | b2970 | 0.51 | +2.8 |
3,110,622 | - | yghF | b2970 | 0.60 | +0.8 |
3,110,622 | - | yghF | b2970 | 0.60 | +1.4 |
3,110,664 | + | yghF | b2970 | 0.65 | +1.2 |
3,110,723 | + | yghF | b2970 | 0.72 | -1.1 |
3,110,732 | + | yghF | b2970 | 0.73 | +0.7 |
3,110,741 | + | yghF | b2970 | 0.74 | +0.2 |
3,110,819 | + | yghF | b2970 | 0.83 | +0.7 |
3,110,826 | - | yghF | b2970 | 0.84 | +0.8 |
3,110,855 | + | yghF | b2970 | 0.87 | +0.4 |
3,110,855 | + | yghF | b2970 | 0.87 | -2.8 |
3,110,855 | + | yghF | b2970 | 0.87 | -0.6 |
3,110,857 | + | yghF | b2970 | 0.87 | -0.4 |
3,110,865 | - | yghF | b2970 | 0.88 | -0.2 |
3,110,877 | + | yghF | b2970 | 0.90 | +0.9 |
3,110,877 | + | yghF | b2970 | 0.90 | +0.3 |
3,110,886 | + | +0.1 | |||
3,110,886 | + | +1.3 | |||
3,110,888 | + | +0.8 | |||
3,110,888 | + | -1.6 | |||
3,110,894 | - | +5.4 | |||
3,110,935 | - | +6.2 | |||
3,110,935 | - | -0.2 | |||
3,110,977 | + | +1.0 | |||
3,110,977 | + | -0.3 | |||
3,110,986 | - | -0.4 | |||
3,110,994 | + | +1.3 | |||
3,110,996 | + | -0.3 | |||
3,111,031 | - | +0.9 | |||
3,111,031 | - | -0.4 | |||
3,111,070 | + | -0.6 | |||
3,111,086 | + | +0.3 | |||
3,111,102 | + | -1.4 | |||
3,111,103 | + | +3.1 | |||
3,111,108 | - | -3.2 | |||
3,111,108 | - | -1.4 | |||
3,111,110 | - | +0.9 | |||
3,111,110 | - | +0.6 | |||
3,111,111 | - | +0.3 | |||
3,111,111 | - | -1.8 | |||
3,111,111 | - | -0.2 | |||
3,111,111 | - | +1.5 | |||
3,111,111 | - | -0.5 | |||
3,111,111 | - | +0.4 | |||
3,111,111 | - | -1.2 | |||
3,111,111 | - | -1.5 | |||
3,111,111 | - | +0.4 | |||
3,111,141 | - | yghG | b2971 | 0.13 | -0.0 |
3,111,141 | - | yghG | b2971 | 0.13 | -2.2 |
3,111,143 | + | yghG | b2971 | 0.13 | +1.7 |
3,111,144 | + | yghG | b2971 | 0.13 | -1.4 |
3,111,145 | - | yghG | b2971 | 0.14 | +0.6 |
3,111,145 | - | yghG | b2971 | 0.14 | -0.1 |
3,111,145 | - | yghG | b2971 | 0.14 | -4.1 |
3,111,145 | - | yghG | b2971 | 0.14 | -0.1 |
3,111,147 | + | yghG | b2971 | 0.14 | +0.6 |
3,111,151 | + | yghG | b2971 | 0.15 | +2.4 |
3,111,152 | - | yghG | b2971 | 0.15 | +0.5 |
3,111,152 | - | yghG | b2971 | 0.15 | -1.8 |
3,111,153 | - | yghG | b2971 | 0.16 | +1.0 |
3,111,155 | - | yghG | b2971 | 0.16 | -0.7 |
3,111,155 | - | yghG | b2971 | 0.16 | -0.4 |
3,111,155 | - | yghG | b2971 | 0.16 | +0.2 |
3,111,178 | - | yghG | b2971 | 0.22 | -0.3 |
3,111,178 | - | yghG | b2971 | 0.22 | +0.2 |
3,111,204 | + | yghG | b2971 | 0.28 | -0.1 |
3,111,204 | + | yghG | b2971 | 0.28 | -2.7 |
3,111,216 | + | yghG | b2971 | 0.31 | +2.1 |
3,111,223 | - | yghG | b2971 | 0.33 | +3.3 |
3,111,223 | - | yghG | b2971 | 0.33 | +0.0 |
3,111,223 | - | yghG | b2971 | 0.33 | +0.7 |
3,111,224 | - | yghG | b2971 | 0.33 | -2.2 |
3,111,224 | - | yghG | b2971 | 0.33 | -0.5 |
3,111,224 | - | yghG | b2971 | 0.33 | -0.9 |
3,111,224 | - | yghG | b2971 | 0.33 | +1.8 |
3,111,224 | - | yghG | b2971 | 0.33 | +1.3 |
3,111,262 | + | yghG | b2971 | 0.42 | -1.8 |
3,111,265 | + | yghG | b2971 | 0.43 | +0.7 |
3,111,265 | + | yghG | b2971 | 0.43 | +0.8 |
3,111,275 | - | yghG | b2971 | 0.45 | -2.3 |
3,111,275 | - | yghG | b2971 | 0.45 | -0.5 |
3,111,329 | + | yghG | b2971 | 0.58 | -1.3 |
3,111,329 | + | yghG | b2971 | 0.58 | -1.5 |
3,111,418 | + | yghG | b2971 | 0.80 | +0.9 |
3,111,450 | - | yghG | b2971 | 0.88 | -1.1 |
3,111,473 | - | -2.8 | |||
3,111,473 | - | +1.1 | |||
3,111,474 | - | +0.2 | |||
3,111,475 | + | +1.7 | |||
3,111,483 | - | -0.3 | |||
3,111,571 | - | +0.5 | |||
3,111,599 | - | +4.8 | |||
3,111,599 | - | -0.7 | |||
3,111,599 | - | -0.5 | |||
3,111,599 | - | -0.5 | |||
3,111,604 | - | +0.9 | |||
3,111,609 | - | +0.5 | |||
3,111,616 | - | +0.7 | |||
3,111,626 | - | +0.4 | |||
3,111,636 | - | -0.7 | |||
3,111,638 | - | -2.3 | |||
3,111,638 | - | -0.8 | |||
3,111,638 | - | +1.2 | |||
3,111,664 | + | b2972 | b2972 | 0.12 | -2.0 |
3,111,664 | + | b2972 | b2972 | 0.12 | -1.6 |
3,111,667 | - | b2972 | b2972 | 0.13 | +1.0 |
3,111,667 | - | b2972 | b2972 | 0.13 | +0.5 |
3,111,671 | - | b2972 | b2972 | 0.13 | -1.0 |
3,111,703 | - | b2972 | b2972 | 0.17 | +0.3 |
3,111,705 | - | b2972 | b2972 | 0.17 | +0.2 |
3,111,705 | - | b2972 | b2972 | 0.17 | +1.9 |
3,111,716 | - | b2972 | b2972 | 0.19 | +0.6 |
3,111,716 | - | b2972 | b2972 | 0.19 | -0.7 |
3,111,724 | + | b2972 | b2972 | 0.20 | +0.1 |
3,111,732 | - | b2972 | b2972 | 0.21 | +0.3 |
3,111,750 | - | b2972 | b2972 | 0.23 | -1.0 |
3,111,754 | + | b2972 | b2972 | 0.23 | -0.9 |
3,111,754 | + | b2972 | b2972 | 0.23 | +1.3 |
3,111,762 | - | b2972 | b2972 | 0.24 | +0.6 |
3,111,762 | - | b2972 | b2972 | 0.24 | -2.8 |
3,111,788 | - | b2972 | b2972 | 0.28 | +0.1 |
3,111,804 | - | b2972 | b2972 | 0.30 | -0.0 |
3,111,823 | + | b2972 | b2972 | 0.32 | -0.1 |
3,111,823 | + | b2972 | b2972 | 0.32 | -3.6 |
3,111,831 | - | b2972 | b2972 | 0.33 | -1.4 |
3,111,834 | - | b2972 | b2972 | 0.33 | +0.1 |
3,111,849 | + | b2972 | b2972 | 0.35 | +0.3 |
3,111,855 | - | b2972 | b2972 | 0.36 | +1.3 |
3,111,857 | - | b2972 | b2972 | 0.36 | +0.5 |
3,111,864 | + | b2972 | b2972 | 0.37 | -0.0 |
3,111,871 | - | b2972 | b2972 | 0.38 | +0.8 |
3,111,878 | - | b2972 | b2972 | 0.39 | -0.9 |
3,111,880 | + | b2972 | b2972 | 0.39 | +0.8 |
3,111,896 | + | b2972 | b2972 | 0.41 | -1.4 |
Or see this region's nucleotide sequence