Strain Fitness in Escherichia coli BW25113 around b2863

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsD-4 and insC overlap by 43 nucleotidesinsC and ygeQ are separated by 91 nucleotidesygeQ and glyU are separated by 155 nucleotidesglyU and b2865 are separated by 78 nucleotides b2860: insD-4 - IS2 insertion element transposase InsAB' (NCBI), at 2,994,394 to 2,995,299 insD-4 b2861: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 2,995,257 to 2,995,622 insC b2863: ygeQ - orf, hypothetical protein (VIMSS), at 2,995,714 to 2,996,850 ygeQ b2864: glyU - tRNA-Gly (NCBI), at 2,997,006 to 2,997,079 glyU b2865: b2865 - putative lipoprotein (VIMSS), at 2,997,158 to 2,997,913 b2865 Position (kb) 2995 2996 2997Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 2995.688 kb on + strandat 2995.688 kb on + strandat 2995.709 kb on + strandat 2995.769 kb on - strandat 2995.769 kb on - strandat 2995.771 kb on + strandat 2995.773 kb on + strandat 2995.801 kb on - strandat 2995.801 kb on - strandat 2995.828 kb on - strand, within ygeQat 2995.828 kb on - strand, within ygeQat 2995.882 kb on - strand, within ygeQat 2995.882 kb on - strand, within ygeQat 2995.882 kb on - strand, within ygeQat 2995.882 kb on - strand, within ygeQat 2995.884 kb on + strand, within ygeQat 2995.885 kb on + strand, within ygeQat 2995.885 kb on + strand, within ygeQat 2995.888 kb on - strand, within ygeQat 2995.907 kb on + strand, within ygeQat 2995.912 kb on + strand, within ygeQat 2995.912 kb on + strand, within ygeQat 2995.924 kb on + strand, within ygeQat 2995.932 kb on - strand, within ygeQat 2995.939 kb on + strand, within ygeQat 2995.939 kb on + strand, within ygeQat 2995.939 kb on + strand, within ygeQat 2995.975 kb on - strand, within ygeQat 2995.975 kb on - strand, within ygeQat 2995.981 kb on + strand, within ygeQat 2995.981 kb on - strand, within ygeQat 2995.992 kb on + strand, within ygeQat 2995.993 kb on + strand, within ygeQat 2995.998 kb on + strand, within ygeQat 2995.998 kb on + strand, within ygeQat 2995.998 kb on + strand, within ygeQat 2996.005 kb on + strand, within ygeQat 2996.006 kb on - strand, within ygeQat 2996.006 kb on - strand, within ygeQat 2996.016 kb on - strand, within ygeQat 2996.017 kb on + strand, within ygeQat 2996.018 kb on - strand, within ygeQat 2996.018 kb on - strand, within ygeQat 2996.038 kb on + strand, within ygeQat 2996.052 kb on - strand, within ygeQat 2996.062 kb on - strand, within ygeQat 2996.080 kb on - strand, within ygeQat 2996.102 kb on - strand, within ygeQat 2996.118 kb on + strand, within ygeQat 2996.118 kb on + strand, within ygeQat 2996.173 kb on - strand, within ygeQat 2996.173 kb on - strand, within ygeQat 2996.173 kb on - strand, within ygeQat 2996.173 kb on - strand, within ygeQat 2996.177 kb on + strand, within ygeQat 2996.177 kb on + strand, within ygeQat 2996.177 kb on + strand, within ygeQat 2996.178 kb on + strand, within ygeQat 2996.180 kb on + strand, within ygeQat 2996.180 kb on + strand, within ygeQat 2996.199 kb on - strand, within ygeQat 2996.200 kb on + strand, within ygeQat 2996.200 kb on + strand, within ygeQat 2996.212 kb on + strand, within ygeQat 2996.212 kb on + strand, within ygeQat 2996.216 kb on + strand, within ygeQat 2996.225 kb on + strand, within ygeQat 2996.225 kb on + strand, within ygeQat 2996.225 kb on - strand, within ygeQat 2996.232 kb on + strand, within ygeQat 2996.232 kb on + strand, within ygeQat 2996.232 kb on + strand, within ygeQat 2996.232 kb on + strand, within ygeQat 2996.247 kb on + strand, within ygeQat 2996.250 kb on + strand, within ygeQat 2996.250 kb on + strand, within ygeQat 2996.250 kb on - strand, within ygeQat 2996.265 kb on + strand, within ygeQat 2996.275 kb on - strand, within ygeQat 2996.281 kb on + strand, within ygeQat 2996.308 kb on - strand, within ygeQat 2996.308 kb on - strand, within ygeQat 2996.331 kb on + strand, within ygeQat 2996.331 kb on + strand, within ygeQat 2996.341 kb on + strand, within ygeQat 2996.341 kb on + strand, within ygeQat 2996.341 kb on + strand, within ygeQat 2996.351 kb on + strand, within ygeQat 2996.352 kb on - strand, within ygeQat 2996.352 kb on - strand, within ygeQat 2996.360 kb on + strand, within ygeQat 2996.364 kb on + strand, within ygeQat 2996.375 kb on + strand, within ygeQat 2996.378 kb on - strand, within ygeQat 2996.378 kb on - strand, within ygeQat 2996.383 kb on - strand, within ygeQat 2996.384 kb on + strand, within ygeQat 2996.391 kb on + strand, within ygeQat 2996.391 kb on + strand, within ygeQat 2996.391 kb on + strand, within ygeQat 2996.391 kb on + strand, within ygeQat 2996.391 kb on + strand, within ygeQat 2996.392 kb on - strand, within ygeQat 2996.392 kb on - strand, within ygeQat 2996.397 kb on + strand, within ygeQat 2996.397 kb on + strand, within ygeQat 2996.397 kb on + strand, within ygeQat 2996.431 kb on + strand, within ygeQat 2996.431 kb on + strand, within ygeQat 2996.461 kb on - strand, within ygeQat 2996.471 kb on + strand, within ygeQat 2996.471 kb on + strand, within ygeQat 2996.482 kb on + strand, within ygeQat 2996.486 kb on + strand, within ygeQat 2996.486 kb on + strand, within ygeQat 2996.494 kb on - strand, within ygeQat 2996.494 kb on - strand, within ygeQat 2996.500 kb on - strand, within ygeQat 2996.554 kb on + strand, within ygeQat 2996.561 kb on + strandat 2996.562 kb on - strand, within ygeQat 2996.621 kb on + strand, within ygeQat 2996.645 kb on + strand, within ygeQat 2996.645 kb on + strand, within ygeQat 2996.765 kb on + strandat 2996.772 kb on - strandat 2996.772 kb on - strandat 2996.775 kb on - strandat 2996.775 kb on - strandat 2996.780 kb on - strandat 2996.785 kb on - strandat 2996.791 kb on - strandat 2996.798 kb on - strandat 2996.798 kb on - strandat 2996.868 kb on - strandat 2996.892 kb on - strandat 2996.949 kb on - strandat 2997.039 kb on - strand, within glyUat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.124 kb on - strandat 2997.320 kb on - strand, within b2865at 2997.320 kb on - strand, within b2865at 2997.358 kb on - strand, within b2865at 2997.364 kb on + strand, within b2865at 2997.364 kb on + strand, within b2865at 2997.372 kb on - strand, within b2865at 2997.379 kb on + strand, within b2865at 2997.379 kb on + strand, within b2865at 2997.413 kb on - strand, within b2865at 2997.413 kb on - strand, within b2865at 2997.429 kb on - strand, within b2865at 2997.429 kb on - strand, within b2865at 2997.435 kb on + strand, within b2865at 2997.437 kb on + strand, within b2865at 2997.444 kb on + strand, within b2865at 2997.450 kb on + strand, within b2865at 2997.450 kb on + strand, within b2865at 2997.452 kb on - strand, within b2865at 2997.525 kb on + strand, within b2865at 2997.537 kb on - strand, within b2865at 2997.553 kb on - strand, within b2865at 2997.587 kb on - strand, within b2865at 2997.602 kb on + strand, within b2865at 2997.632 kb on + strand, within b2865at 2997.696 kb on + strand, within b2865at 2997.698 kb on - strand, within b2865at 2997.700 kb on + strand, within b2865at 2997.727 kb on + strand, within b2865at 2997.727 kb on + strand, within b2865at 2997.738 kb on + strand, within b2865at 2997.754 kb on + strand, within b2865at 2997.759 kb on + strand, within b2865at 2997.759 kb on + strand, within b2865at 2997.761 kb on + strand, within b2865at 2997.789 kb on - strand, within b2865at 2997.794 kb on - strand, within b2865at 2997.795 kb on - strand, within b2865at 2997.796 kb on - strand, within b2865at 2997.830 kb on - strand, within b2865

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,995,688 + +1.6
2,995,688 + -2.5
2,995,709 + +0.8
2,995,769 - -1.1
2,995,769 - +0.9
2,995,771 + +3.4
2,995,773 + +1.4
2,995,801 - -0.3
2,995,801 - +0.1
2,995,828 - ygeQ b2863 0.10 -0.3
2,995,828 - ygeQ b2863 0.10 -1.2
2,995,882 - ygeQ b2863 0.15 +0.7
2,995,882 - ygeQ b2863 0.15 +1.7
2,995,882 - ygeQ b2863 0.15 -3.1
2,995,882 - ygeQ b2863 0.15 -0.3
2,995,884 + ygeQ b2863 0.15 -1.4
2,995,885 + ygeQ b2863 0.15 -1.8
2,995,885 + ygeQ b2863 0.15 +0.0
2,995,888 - ygeQ b2863 0.15 +0.4
2,995,907 + ygeQ b2863 0.17 -0.1
2,995,912 + ygeQ b2863 0.17 +1.2
2,995,912 + ygeQ b2863 0.17 +0.9
2,995,924 + ygeQ b2863 0.18 -2.1
2,995,932 - ygeQ b2863 0.19 +2.0
2,995,939 + ygeQ b2863 0.20 -0.2
2,995,939 + ygeQ b2863 0.20 -0.6
2,995,939 + ygeQ b2863 0.20 +0.6
2,995,975 - ygeQ b2863 0.23 -2.4
2,995,975 - ygeQ b2863 0.23 -0.1
2,995,981 + ygeQ b2863 0.23 -3.2
2,995,981 - ygeQ b2863 0.23 +0.9
2,995,992 + ygeQ b2863 0.24 +1.8
2,995,993 + ygeQ b2863 0.25 +1.7
2,995,998 + ygeQ b2863 0.25 +0.0
2,995,998 + ygeQ b2863 0.25 -0.2
2,995,998 + ygeQ b2863 0.25 +3.2
2,996,005 + ygeQ b2863 0.26 +1.0
2,996,006 - ygeQ b2863 0.26 -0.6
2,996,006 - ygeQ b2863 0.26 -0.6
2,996,016 - ygeQ b2863 0.27 -0.4
2,996,017 + ygeQ b2863 0.27 -0.7
2,996,018 - ygeQ b2863 0.27 +1.2
2,996,018 - ygeQ b2863 0.27 +1.1
2,996,038 + ygeQ b2863 0.28 -0.7
2,996,052 - ygeQ b2863 0.30 +1.4
2,996,062 - ygeQ b2863 0.31 -0.3
2,996,080 - ygeQ b2863 0.32 +1.9
2,996,102 - ygeQ b2863 0.34 -3.4
2,996,118 + ygeQ b2863 0.36 -1.5
2,996,118 + ygeQ b2863 0.36 +3.2
2,996,173 - ygeQ b2863 0.40 +1.3
2,996,173 - ygeQ b2863 0.40 +1.8
2,996,173 - ygeQ b2863 0.40 -2.5
2,996,173 - ygeQ b2863 0.40 +0.0
2,996,177 + ygeQ b2863 0.41 +0.3
2,996,177 + ygeQ b2863 0.41 +1.4
2,996,177 + ygeQ b2863 0.41 +0.2
2,996,178 + ygeQ b2863 0.41 +1.4
2,996,180 + ygeQ b2863 0.41 +1.1
2,996,180 + ygeQ b2863 0.41 +0.8
2,996,199 - ygeQ b2863 0.43 +0.2
2,996,200 + ygeQ b2863 0.43 -0.2
2,996,200 + ygeQ b2863 0.43 -1.4
2,996,212 + ygeQ b2863 0.44 +1.6
2,996,212 + ygeQ b2863 0.44 -2.2
2,996,216 + ygeQ b2863 0.44 +2.4
2,996,225 + ygeQ b2863 0.45 +0.1
2,996,225 + ygeQ b2863 0.45 +0.5
2,996,225 - ygeQ b2863 0.45 +0.5
2,996,232 + ygeQ b2863 0.46 -0.5
2,996,232 + ygeQ b2863 0.46 -0.1
2,996,232 + ygeQ b2863 0.46 +3.3
2,996,232 + ygeQ b2863 0.46 -2.2
2,996,247 + ygeQ b2863 0.47 -0.1
2,996,250 + ygeQ b2863 0.47 -0.5
2,996,250 + ygeQ b2863 0.47 -0.2
2,996,250 - ygeQ b2863 0.47 +0.9
2,996,265 + ygeQ b2863 0.48 +1.0
2,996,275 - ygeQ b2863 0.49 +1.1
2,996,281 + ygeQ b2863 0.50 +0.8
2,996,308 - ygeQ b2863 0.52 -0.6
2,996,308 - ygeQ b2863 0.52 +0.5
2,996,331 + ygeQ b2863 0.54 -2.7
2,996,331 + ygeQ b2863 0.54 -1.5
2,996,341 + ygeQ b2863 0.55 +1.6
2,996,341 + ygeQ b2863 0.55 +0.9
2,996,341 + ygeQ b2863 0.55 -0.2
2,996,351 + ygeQ b2863 0.56 -0.0
2,996,352 - ygeQ b2863 0.56 -0.3
2,996,352 - ygeQ b2863 0.56 +2.1
2,996,360 + ygeQ b2863 0.57 -1.0
2,996,364 + ygeQ b2863 0.57 +0.5
2,996,375 + ygeQ b2863 0.58 -1.4
2,996,378 - ygeQ b2863 0.58 -3.2
2,996,378 - ygeQ b2863 0.58 -1.4
2,996,383 - ygeQ b2863 0.59 +0.3
2,996,384 + ygeQ b2863 0.59 -0.6
2,996,391 + ygeQ b2863 0.60 -0.7
2,996,391 + ygeQ b2863 0.60 +0.3
2,996,391 + ygeQ b2863 0.60 +3.9
2,996,391 + ygeQ b2863 0.60 +1.9
2,996,391 + ygeQ b2863 0.60 +0.2
2,996,392 - ygeQ b2863 0.60 +2.1
2,996,392 - ygeQ b2863 0.60 +0.6
2,996,397 + ygeQ b2863 0.60 +0.3
2,996,397 + ygeQ b2863 0.60 +3.5
2,996,397 + ygeQ b2863 0.60 -1.1
2,996,431 + ygeQ b2863 0.63 +1.3
2,996,431 + ygeQ b2863 0.63 +0.3
2,996,461 - ygeQ b2863 0.66 +1.1
2,996,471 + ygeQ b2863 0.67 -1.4
2,996,471 + ygeQ b2863 0.67 +0.8
2,996,482 + ygeQ b2863 0.68 +0.7
2,996,486 + ygeQ b2863 0.68 +0.9
2,996,486 + ygeQ b2863 0.68 -1.1
2,996,494 - ygeQ b2863 0.69 +0.2
2,996,494 - ygeQ b2863 0.69 +4.8
2,996,500 - ygeQ b2863 0.69 +0.5
2,996,554 + ygeQ b2863 0.74 -0.9
2,996,561 + +1.0
2,996,562 - ygeQ b2863 0.75 +1.2
2,996,621 + ygeQ b2863 0.80 +0.9
2,996,645 + ygeQ b2863 0.82 -2.7
2,996,645 + ygeQ b2863 0.82 +0.7
2,996,765 + -2.8
2,996,772 - +1.3
2,996,772 - -3.3
2,996,775 - -0.6
2,996,775 - +4.2
2,996,780 - -1.1
2,996,785 - -2.3
2,996,791 - -2.7
2,996,798 - -0.7
2,996,798 - -1.3
2,996,868 - -0.2
2,996,892 - +0.2
2,996,949 - -1.2
2,997,039 - glyU b2864 0.45 +0.1
2,997,124 - +0.0
2,997,124 - -0.1
2,997,124 - +1.5
2,997,124 - -1.9
2,997,124 - -1.3
2,997,124 - +1.5
2,997,124 - +0.0
2,997,124 - +1.0
2,997,124 - -0.2
2,997,320 - b2865 b2865 0.21 -3.3
2,997,320 - b2865 b2865 0.21 -0.4
2,997,358 - b2865 b2865 0.26 +2.3
2,997,364 + b2865 b2865 0.27 -1.6
2,997,364 + b2865 b2865 0.27 +0.1
2,997,372 - b2865 b2865 0.28 -1.2
2,997,379 + b2865 b2865 0.29 +1.2
2,997,379 + b2865 b2865 0.29 +1.3
2,997,413 - b2865 b2865 0.34 +1.1
2,997,413 - b2865 b2865 0.34 +0.5
2,997,429 - b2865 b2865 0.36 -0.4
2,997,429 - b2865 b2865 0.36 +1.0
2,997,435 + b2865 b2865 0.37 +4.3
2,997,437 + b2865 b2865 0.37 +1.0
2,997,444 + b2865 b2865 0.38 +0.1
2,997,450 + b2865 b2865 0.39 +1.9
2,997,450 + b2865 b2865 0.39 -2.3
2,997,452 - b2865 b2865 0.39 +1.1
2,997,525 + b2865 b2865 0.49 +1.7
2,997,537 - b2865 b2865 0.50 +0.7
2,997,553 - b2865 b2865 0.52 +0.7
2,997,587 - b2865 b2865 0.57 +3.7
2,997,602 + b2865 b2865 0.59 -0.1
2,997,632 + b2865 b2865 0.63 -0.8
2,997,696 + b2865 b2865 0.71 -3.2
2,997,698 - b2865 b2865 0.71 +0.6
2,997,700 + b2865 b2865 0.72 -0.5
2,997,727 + b2865 b2865 0.75 +0.8
2,997,727 + b2865 b2865 0.75 +2.1
2,997,738 + b2865 b2865 0.77 -0.8
2,997,754 + b2865 b2865 0.79 +0.3
2,997,759 + b2865 b2865 0.79 -0.5
2,997,759 + b2865 b2865 0.79 +0.5
2,997,761 + b2865 b2865 0.80 -2.2
2,997,789 - b2865 b2865 0.83 +0.2
2,997,794 - b2865 b2865 0.84 +0.4
2,997,795 - b2865 b2865 0.84 +1.5
2,997,796 - b2865 b2865 0.84 +0.2
2,997,830 - b2865 b2865 0.89 +0.8

Or see this region's nucleotide sequence