Strain Fitness in Escherichia coli BW25113 around b1523

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyneF and yneG are separated by 111 nucleotidesyneG and yneH overlap by 1 nucleotidesyneH and b1525 are separated by 63 nucleotides b1522: yneF - predicted diguanylate cyclase (NCBI), at 1,608,931 to 1,609,878 yneF b1523: yneG - hypothetical protein (NCBI), at 1,609,990 to 1,610,349 yneG b1524: yneH - predicted glutaminase (NCBI), at 1,610,349 to 1,611,275 yneH b1525: b1525 - putative aldehyde dehydrogenase (VIMSS), at 1,611,339 to 1,612,727 b1525 Position (kb) 1609 1610 1611Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1608.994 kb on + strandat 1609.041 kb on - strand, within yneFat 1609.234 kb on - strand, within yneFat 1609.408 kb on - strand, within yneFat 1609.728 kb on - strand, within yneFat 1609.730 kb on - strand, within yneFat 1609.859 kb on - strandat 1609.946 kb on + strandat 1610.016 kb on + strandat 1610.047 kb on - strand, within yneGat 1610.249 kb on + strand, within yneGat 1610.422 kb on - strandat 1610.543 kb on + strand, within yneHat 1611.288 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,608,994 + +0.3
1,609,041 - yneF b1522 0.12 -0.7
1,609,234 - yneF b1522 0.32 -1.3
1,609,408 - yneF b1522 0.50 -1.3
1,609,728 - yneF b1522 0.84 +0.4
1,609,730 - yneF b1522 0.84 +1.0
1,609,859 - -3.5
1,609,946 + +2.5
1,610,016 + +0.8
1,610,047 - yneG b1523 0.16 +1.1
1,610,249 + yneG b1523 0.72 +1.0
1,610,422 - +2.6
1,610,543 + yneH b1524 0.21 -2.0
1,611,288 + -1.9

Or see this region's nucleotide sequence