Strain Fitness in Escherichia coli BW25113 around b1409

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydbD and b1408 are separated by 170 nucleotidesb1408 and ynbB overlap by 1 nucleotidesynbB and ynbC are separated by 15 nucleotides b1407: ydbD - orf, hypothetical protein (VIMSS), at 1,473,168 to 1,475,474 ydbD b1408: b1408 - probable enzyme (VIMSS), at 1,475,645 to 1,476,250 b1408 b1409: ynbB - predicted CDP-diglyceride synthase (NCBI), at 1,476,250 to 1,477,146 ynbB b1410: ynbC - predicted hydrolase (NCBI), at 1,477,162 to 1,478,919 ynbC Position (kb) 1476 1477 1478Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1475.254 kb on + strandat 1475.348 kb on + strandat 1475.383 kb on - strandat 1475.610 kb on - strandat 1475.691 kb on - strandat 1475.719 kb on + strand, within b1408at 1475.771 kb on + strand, within b1408at 1475.771 kb on + strand, within b1408at 1475.790 kb on + strand, within b1408at 1475.838 kb on + strand, within b1408at 1475.838 kb on + strand, within b1408at 1475.850 kb on + strand, within b1408at 1475.850 kb on + strand, within b1408at 1475.862 kb on + strand, within b1408at 1475.862 kb on + strand, within b1408at 1475.866 kb on + strand, within b1408at 1475.866 kb on + strand, within b1408at 1475.866 kb on - strand, within b1408at 1475.903 kb on + strand, within b1408at 1476.147 kb on + strand, within b1408at 1476.147 kb on + strand, within b1408at 1476.882 kb on - strand, within ynbBat 1476.882 kb on - strand, within ynbBat 1476.894 kb on - strand, within ynbBat 1476.894 kb on - strand, within ynbBat 1476.902 kb on + strand, within ynbBat 1476.924 kb on + strand, within ynbBat 1476.934 kb on + strand, within ynbBat 1476.934 kb on + strand, within ynbBat 1477.000 kb on + strand, within ynbBat 1477.008 kb on + strand, within ynbBat 1477.055 kb on + strand, within ynbBat 1477.064 kb on - strandat 1477.066 kb on + strandat 1477.074 kb on + strandat 1477.088 kb on + strandat 1477.214 kb on - strandat 1477.256 kb on - strandat 1477.265 kb on - strandat 1477.317 kb on - strandat 1477.333 kb on - strandat 1477.397 kb on + strand, within ynbCat 1477.401 kb on - strand, within ynbCat 1477.563 kb on - strand, within ynbCat 1477.563 kb on - strand, within ynbCat 1477.624 kb on - strand, within ynbCat 1477.688 kb on - strand, within ynbCat 1477.781 kb on - strand, within ynbCat 1477.859 kb on - strand, within ynbCat 1478.058 kb on + strand, within ynbC

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,475,254 + -0.8
1,475,348 + -1.0
1,475,383 - +2.3
1,475,610 - -0.8
1,475,691 - +0.9
1,475,719 + b1408 b1408 0.12 -0.7
1,475,771 + b1408 b1408 0.21 -0.4
1,475,771 + b1408 b1408 0.21 +1.9
1,475,790 + b1408 b1408 0.24 +0.8
1,475,838 + b1408 b1408 0.32 -0.7
1,475,838 + b1408 b1408 0.32 +0.4
1,475,850 + b1408 b1408 0.34 +0.4
1,475,850 + b1408 b1408 0.34 -1.3
1,475,862 + b1408 b1408 0.36 +1.3
1,475,862 + b1408 b1408 0.36 -0.3
1,475,866 + b1408 b1408 0.36 -1.2
1,475,866 + b1408 b1408 0.36 +1.4
1,475,866 - b1408 b1408 0.36 -1.7
1,475,903 + b1408 b1408 0.43 +1.7
1,476,147 + b1408 b1408 0.83 -0.9
1,476,147 + b1408 b1408 0.83 -0.7
1,476,882 - ynbB b1409 0.70 -2.7
1,476,882 - ynbB b1409 0.70 -2.9
1,476,894 - ynbB b1409 0.72 -1.5
1,476,894 - ynbB b1409 0.72 -1.6
1,476,902 + ynbB b1409 0.73 -0.2
1,476,924 + ynbB b1409 0.75 +1.7
1,476,934 + ynbB b1409 0.76 -2.2
1,476,934 + ynbB b1409 0.76 -0.1
1,477,000 + ynbB b1409 0.84 -0.0
1,477,008 + ynbB b1409 0.85 -2.0
1,477,055 + ynbB b1409 0.90 +1.3
1,477,064 - -3.6
1,477,066 + +2.1
1,477,074 + +0.3
1,477,088 + +0.2
1,477,214 - -4.3
1,477,256 - -0.5
1,477,265 - +1.9
1,477,317 - +0.5
1,477,333 - +1.2
1,477,397 + ynbC b1410 0.13 +1.7
1,477,401 - ynbC b1410 0.14 +1.3
1,477,563 - ynbC b1410 0.23 +1.9
1,477,563 - ynbC b1410 0.23 +0.7
1,477,624 - ynbC b1410 0.26 -1.4
1,477,688 - ynbC b1410 0.30 -0.0
1,477,781 - ynbC b1410 0.35 +0.3
1,477,859 - ynbC b1410 0.40 -0.1
1,478,058 + ynbC b1410 0.51 +2.0

Or see this region's nucleotide sequence