Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdI and rarA are separated by 9 nucleotides rarA and ycdK are separated by 7 nucleotides ycdK and b1011 are separated by 11 nucleotides b1011 and b1012 overlap by 1 nucleotides
b1008: ycdI - hypothetical protein (NCBI), at 1,069,588 to 1,070,178
ycdI
b1009: rarA - predicted hydrolase (NCBI), at 1,070,188 to 1,070,988
rarA
b1010: ycdK - hypothetical protein (NCBI), at 1,070,996 to 1,071,382
ycdK
b1011: b1011 - putative synthetase (VIMSS), at 1,071,394 to 1,072,086
b1011
b1012: b1012 - orf, hypothetical protein (VIMSS), at 1,072,086 to 1,073,234
b1012
Position (kb)
1070
1071
1072 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1070.145 kb on - strand at 1070.145 kb on - strand at 1070.152 kb on - strand at 1070.241 kb on - strand at 1070.241 kb on - strand at 1070.569 kb on - strand, within rarA at 1070.580 kb on - strand, within rarA at 1070.580 kb on - strand, within rarA at 1070.633 kb on - strand, within rarA at 1070.633 kb on - strand, within rarA at 1070.678 kb on - strand, within rarA at 1070.684 kb on + strand, within rarA at 1070.689 kb on + strand, within rarA at 1070.840 kb on + strand, within rarA at 1070.863 kb on - strand, within rarA at 1070.875 kb on - strand, within rarA at 1070.875 kb on - strand, within rarA at 1070.956 kb on + strand at 1070.956 kb on + strand at 1070.988 kb on + strand at 1071.230 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.319 kb on - strand, within ycdK at 1071.343 kb on - strand, within ycdK at 1071.343 kb on - strand, within ycdK at 1071.382 kb on + strand at 1071.696 kb on + strand, within b1011 at 1071.712 kb on + strand, within b1011 at 1071.737 kb on - strand, within b1011 at 1072.068 kb on + strand at 1072.068 kb on + strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.188 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,070,145 - +0.9 1,070,145 - +0.0 1,070,152 - -2.7 1,070,241 - -0.1 1,070,241 - -1.6 1,070,569 - rarA b1009 0.48 -1.4 1,070,580 - rarA b1009 0.49 +1.0 1,070,580 - rarA b1009 0.49 +1.2 1,070,633 - rarA b1009 0.56 +2.1 1,070,633 - rarA b1009 0.56 +0.7 1,070,678 - rarA b1009 0.61 +1.7 1,070,684 + rarA b1009 0.62 -0.8 1,070,689 + rarA b1009 0.63 +2.7 1,070,840 + rarA b1009 0.81 +0.3 1,070,863 - rarA b1009 0.84 -0.2 1,070,875 - rarA b1009 0.86 -1.3 1,070,875 - rarA b1009 0.86 -0.1 1,070,956 + -0.1 1,070,956 + +0.2 1,070,988 + -1.7 1,071,230 - ycdK b1010 0.60 +0.5 1,071,301 - ycdK b1010 0.79 +2.5 1,071,301 - ycdK b1010 0.79 +0.5 1,071,301 - ycdK b1010 0.79 +4.2 1,071,319 - ycdK b1010 0.83 +1.2 1,071,343 - ycdK b1010 0.90 -1.0 1,071,343 - ycdK b1010 0.90 +0.1 1,071,382 + +1.0 1,071,696 + b1011 b1011 0.44 +1.6 1,071,712 + b1011 b1011 0.46 -0.1 1,071,737 - b1011 b1011 0.49 +0.8 1,072,068 + +2.8 1,072,068 + -0.1 1,072,104 - -2.0 1,072,104 - +1.8 1,072,188 + +0.4
Or see this region's nucleotide sequence