Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpcA and yafJ are separated by 205 nucleotides yafJ and yafK overlap by 30 nucleotides yafK and yafQ are separated by 155 nucleotides yafQ and dinJ are separated by 2 nucleotides dinJ and yafL are separated by 209 nucleotides
b0222: lpcA - phosphoheptose isomerase (NCBI), at 243,543 to 244,121
lpcA
b0223: yafJ - predicted amidotransfease (NCBI), at 244,327 to 245,094
yafJ
b0224: yafK - hypothetical protein (NCBI), at 245,065 to 245,805
yafK
b0225: yafQ - predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI), at 245,961 to 246,239
yafQ
b0226: dinJ - predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI), at 246,242 to 246,502
dinJ
b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461
yafL
Position (kb) 245
246 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 244.070 kb on - strand at 244.078 kb on + strand at 244.080 kb on - strand at 244.124 kb on - strand at 244.129 kb on - strand at 244.159 kb on + strand at 244.197 kb on + strand at 244.217 kb on - strand at 244.311 kb on + strand at 244.311 kb on + strand at 244.319 kb on - strand at 244.371 kb on - strand at 244.396 kb on - strand at 244.396 kb on - strand at 244.442 kb on + strand, within yafJ at 244.453 kb on + strand, within yafJ at 244.516 kb on + strand, within yafJ at 244.551 kb on + strand, within yafJ at 244.564 kb on - strand, within yafJ at 244.564 kb on - strand, within yafJ at 244.616 kb on + strand, within yafJ at 244.616 kb on + strand, within yafJ at 244.637 kb on - strand, within yafJ at 244.728 kb on + strand, within yafJ at 244.763 kb on - strand, within yafJ at 244.799 kb on - strand, within yafJ at 244.799 kb on - strand, within yafJ at 244.868 kb on + strand, within yafJ at 245.001 kb on - strand, within yafJ at 245.001 kb on - strand, within yafJ at 245.011 kb on - strand, within yafJ at 245.083 kb on + strand at 245.117 kb on - strand at 245.175 kb on + strand, within yafK at 245.187 kb on + strand, within yafK at 245.207 kb on + strand, within yafK at 245.278 kb on - strand, within yafK at 245.278 kb on - strand, within yafK at 245.285 kb on + strand, within yafK at 245.296 kb on - strand, within yafK at 245.315 kb on - strand, within yafK at 245.315 kb on - strand, within yafK at 245.355 kb on - strand, within yafK at 245.357 kb on - strand, within yafK at 245.388 kb on - strand, within yafK at 245.388 kb on - strand, within yafK at 245.407 kb on + strand, within yafK at 245.431 kb on - strand, within yafK at 245.448 kb on - strand, within yafK at 245.480 kb on - strand, within yafK at 245.524 kb on - strand, within yafK at 245.524 kb on - strand, within yafK at 245.526 kb on + strand, within yafK at 245.611 kb on + strand, within yafK at 245.611 kb on + strand, within yafK at 245.671 kb on + strand, within yafK at 245.679 kb on - strand, within yafK at 245.704 kb on - strand, within yafK at 245.704 kb on - strand at 245.706 kb on - strand, within yafK at 245.706 kb on - strand, within yafK at 245.706 kb on - strand, within yafK at 245.735 kb on - strand at 245.743 kb on - strand at 245.743 kb on - strand at 245.837 kb on + strand at 245.928 kb on - strand at 245.928 kb on - strand at 245.964 kb on + strand at 245.964 kb on + strand at 245.971 kb on - strand at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.097 kb on - strand, within yafQ at 246.294 kb on - strand, within dinJ at 246.295 kb on - strand, within dinJ at 246.304 kb on - strand, within dinJ at 246.330 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 244,070 - -2.1 244,078 + -2.8 244,080 - +0.3 244,124 - +2.8 244,129 - +0.3 244,159 + +3.6 244,197 + +0.3 244,217 - +0.5 244,311 + +0.6 244,311 + -2.3 244,319 - +0.4 244,371 - +1.0 244,396 - -1.3 244,396 - +0.6 244,442 + yafJ b0223 0.15 +1.2 244,453 + yafJ b0223 0.16 -0.8 244,516 + yafJ b0223 0.25 +0.5 244,551 + yafJ b0223 0.29 +2.0 244,564 - yafJ b0223 0.31 +1.0 244,564 - yafJ b0223 0.31 -0.6 244,616 + yafJ b0223 0.38 -1.5 244,616 + yafJ b0223 0.38 +1.3 244,637 - yafJ b0223 0.40 -0.1 244,728 + yafJ b0223 0.52 +2.2 244,763 - yafJ b0223 0.57 +0.9 244,799 - yafJ b0223 0.61 -0.9 244,799 - yafJ b0223 0.61 +0.7 244,868 + yafJ b0223 0.70 +0.4 245,001 - yafJ b0223 0.88 +2.2 245,001 - yafJ b0223 0.88 +1.1 245,011 - yafJ b0223 0.89 +0.1 245,083 + +1.2 245,117 - +0.5 245,175 + yafK b0224 0.15 +0.4 245,187 + yafK b0224 0.16 +0.4 245,207 + yafK b0224 0.19 +1.3 245,278 - yafK b0224 0.29 +0.0 245,278 - yafK b0224 0.29 +3.9 245,285 + yafK b0224 0.30 +0.6 245,296 - yafK b0224 0.31 +2.2 245,315 - yafK b0224 0.34 +2.4 245,315 - yafK b0224 0.34 +3.7 245,355 - yafK b0224 0.39 +0.6 245,357 - yafK b0224 0.39 +1.0 245,388 - yafK b0224 0.44 +1.2 245,388 - yafK b0224 0.44 +0.6 245,407 + yafK b0224 0.46 -0.8 245,431 - yafK b0224 0.49 -0.3 245,448 - yafK b0224 0.52 +2.8 245,480 - yafK b0224 0.56 +0.8 245,524 - yafK b0224 0.62 +0.1 245,524 - yafK b0224 0.62 -0.2 245,526 + yafK b0224 0.62 +0.9 245,611 + yafK b0224 0.74 +1.2 245,611 + yafK b0224 0.74 +1.9 245,671 + yafK b0224 0.82 +1.0 245,679 - yafK b0224 0.83 +0.4 245,704 - yafK b0224 0.86 +3.2 245,704 - +4.0 245,706 - yafK b0224 0.87 +1.3 245,706 - yafK b0224 0.87 -2.3 245,706 - yafK b0224 0.87 -2.8 245,735 - +1.9 245,743 - -3.3 245,743 - +0.1 245,837 + +0.8 245,928 - -0.2 245,928 - -2.6 245,964 + +1.3 245,964 + -0.2 245,971 - +1.6 246,048 + yafQ b0225 0.31 +0.4 246,048 + yafQ b0225 0.31 -1.1 246,048 + yafQ b0225 0.31 +3.0 246,086 - yafQ b0225 0.45 -0.1 246,086 - yafQ b0225 0.45 +1.1 246,086 - yafQ b0225 0.45 +0.2 246,086 - yafQ b0225 0.45 -0.4 246,097 - yafQ b0225 0.49 +1.4 246,294 - dinJ b0226 0.20 +2.3 246,295 - dinJ b0226 0.20 +1.5 246,304 - dinJ b0226 0.24 -0.4 246,330 - dinJ b0226 0.34 -1.4 246,338 - dinJ b0226 0.37 +1.5 246,338 - dinJ b0226 0.37 -0.1 246,411 + dinJ b0226 0.65 -2.8 246,411 + dinJ b0226 0.65 -0.9 246,742 - +2.9
Or see this region's nucleotide sequence