Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argH and oxyS are separated by 61 nucleotides oxyS and oxyR are separated by 95 nucleotides
b3960: argH - argininosuccinate lyase (NCBI), at 4,154,873 to 4,156,246
argH
b4458: oxyS - global regulatory RNA (NCBI), at 4,156,308 to 4,156,417
oxyS
b3961: oxyR - DNA-binding transcriptional dual regulator (NCBI), at 4,156,513 to 4,157,430
oxyR
b3962: udhA - putative oxidoreductase (VIMSS), at 4,157,413 to 4,158,813
udhA
Position (kb)
4156
4157 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4155.336 kb on - strand, within argH at 4155.338 kb on + strand, within argH at 4155.366 kb on - strand, within argH at 4155.366 kb on - strand, within argH at 4155.373 kb on + strand, within argH at 4155.381 kb on - strand, within argH at 4155.385 kb on - strand, within argH at 4155.436 kb on - strand, within argH at 4155.437 kb on + strand, within argH at 4155.437 kb on + strand, within argH at 4155.619 kb on - strand, within argH at 4155.699 kb on - strand, within argH at 4155.699 kb on - strand, within argH at 4155.713 kb on - strand, within argH at 4155.713 kb on - strand, within argH at 4155.767 kb on + strand, within argH at 4155.767 kb on + strand, within argH at 4155.770 kb on - strand, within argH at 4155.770 kb on - strand, within argH at 4155.865 kb on + strand, within argH at 4155.875 kb on + strand, within argH at 4155.876 kb on + strand, within argH at 4155.883 kb on - strand, within argH at 4155.885 kb on + strand, within argH at 4155.885 kb on + strand, within argH at 4155.958 kb on + strand, within argH at 4155.961 kb on - strand, within argH at 4156.012 kb on + strand, within argH at 4156.024 kb on + strand, within argH at 4156.024 kb on + strand, within argH at 4156.034 kb on - strand, within argH at 4156.046 kb on + strand, within argH at 4156.054 kb on - strand, within argH at 4156.117 kb on + strand at 4156.156 kb on + strand at 4156.156 kb on + strand at 4156.196 kb on + strand at 4156.196 kb on + strand at 4156.215 kb on - strand at 4156.235 kb on + strand at 4156.239 kb on - strand at 4156.322 kb on - strand, within oxyS at 4156.322 kb on - strand, within oxyS at 4156.476 kb on - strand at 4156.476 kb on - strand at 4156.476 kb on - strand at 4156.517 kb on - strand at 4156.555 kb on - strand at 4156.633 kb on - strand, within oxyR at 4156.633 kb on - strand, within oxyR at 4156.667 kb on + strand, within oxyR at 4156.703 kb on + strand, within oxyR at 4156.703 kb on + strand, within oxyR at 4156.750 kb on - strand, within oxyR at 4156.757 kb on + strand, within oxyR at 4156.775 kb on - strand, within oxyR at 4156.841 kb on + strand, within oxyR at 4156.841 kb on + strand, within oxyR at 4156.886 kb on + strand, within oxyR at 4156.886 kb on + strand, within oxyR at 4156.899 kb on - strand, within oxyR at 4156.926 kb on - strand, within oxyR at 4156.926 kb on - strand, within oxyR at 4157.000 kb on + strand, within oxyR at 4157.001 kb on + strand, within oxyR at 4157.021 kb on + strand, within oxyR at 4157.031 kb on + strand, within oxyR at 4157.050 kb on + strand, within oxyR at 4157.156 kb on + strand, within oxyR at 4157.166 kb on + strand, within oxyR at 4157.166 kb on + strand, within oxyR at 4157.180 kb on + strand, within oxyR at 4157.204 kb on - strand, within oxyR at 4157.213 kb on + strand, within oxyR at 4157.213 kb on + strand, within oxyR at 4157.230 kb on - strand, within oxyR at 4157.283 kb on + strand, within oxyR at 4157.291 kb on - strand, within oxyR at 4157.330 kb on - strand, within oxyR at 4157.338 kb on - strand, within oxyR at 4157.341 kb on + strand at 4157.349 kb on - strand at 4157.354 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,155,336 - argH b3960 0.34 +0.5 4,155,338 + argH b3960 0.34 -0.9 4,155,366 - argH b3960 0.36 +0.1 4,155,366 - argH b3960 0.36 -0.8 4,155,373 + argH b3960 0.36 +0.5 4,155,381 - argH b3960 0.37 +1.5 4,155,385 - argH b3960 0.37 +0.1 4,155,436 - argH b3960 0.41 +1.1 4,155,437 + argH b3960 0.41 -1.6 4,155,437 + argH b3960 0.41 +0.4 4,155,619 - argH b3960 0.54 +0.1 4,155,699 - argH b3960 0.60 +1.0 4,155,699 - argH b3960 0.60 +0.4 4,155,713 - argH b3960 0.61 -1.9 4,155,713 - argH b3960 0.61 +0.5 4,155,767 + argH b3960 0.65 -0.3 4,155,767 + argH b3960 0.65 -3.2 4,155,770 - argH b3960 0.65 -1.8 4,155,770 - argH b3960 0.65 +2.0 4,155,865 + argH b3960 0.72 -2.4 4,155,875 + argH b3960 0.73 -0.6 4,155,876 + argH b3960 0.73 -2.6 4,155,883 - argH b3960 0.74 +0.1 4,155,885 + argH b3960 0.74 -1.8 4,155,885 + argH b3960 0.74 -0.7 4,155,958 + argH b3960 0.79 +0.9 4,155,961 - argH b3960 0.79 -0.2 4,156,012 + argH b3960 0.83 +2.1 4,156,024 + argH b3960 0.84 -0.1 4,156,024 + argH b3960 0.84 +1.9 4,156,034 - argH b3960 0.84 +0.3 4,156,046 + argH b3960 0.85 +0.5 4,156,054 - argH b3960 0.86 -0.8 4,156,117 + +1.1 4,156,156 + -1.8 4,156,156 + +0.5 4,156,196 + +0.1 4,156,196 + +0.7 4,156,215 - -0.1 4,156,235 + +0.1 4,156,239 - -0.1 4,156,322 - oxyS b4458 0.13 -2.3 4,156,322 - oxyS b4458 0.13 -0.7 4,156,476 - -1.4 4,156,476 - -0.2 4,156,476 - +0.9 4,156,517 - +1.9 4,156,555 - +0.3 4,156,633 - oxyR b3961 0.13 +0.7 4,156,633 - oxyR b3961 0.13 -0.7 4,156,667 + oxyR b3961 0.17 +1.0 4,156,703 + oxyR b3961 0.21 +0.3 4,156,703 + oxyR b3961 0.21 -2.5 4,156,750 - oxyR b3961 0.26 -0.4 4,156,757 + oxyR b3961 0.27 -1.9 4,156,775 - oxyR b3961 0.29 -1.2 4,156,841 + oxyR b3961 0.36 +0.0 4,156,841 + oxyR b3961 0.36 -0.7 4,156,886 + oxyR b3961 0.41 -1.4 4,156,886 + oxyR b3961 0.41 +1.0 4,156,899 - oxyR b3961 0.42 -0.5 4,156,926 - oxyR b3961 0.45 +0.3 4,156,926 - oxyR b3961 0.45 -1.3 4,157,000 + oxyR b3961 0.53 -1.1 4,157,001 + oxyR b3961 0.53 -0.8 4,157,021 + oxyR b3961 0.55 +0.1 4,157,031 + oxyR b3961 0.56 -2.8 4,157,050 + oxyR b3961 0.58 +0.6 4,157,156 + oxyR b3961 0.70 +0.1 4,157,166 + oxyR b3961 0.71 -0.5 4,157,166 + oxyR b3961 0.71 +0.4 4,157,180 + oxyR b3961 0.73 -0.2 4,157,204 - oxyR b3961 0.75 -1.6 4,157,213 + oxyR b3961 0.76 +1.0 4,157,213 + oxyR b3961 0.76 +0.0 4,157,230 - oxyR b3961 0.78 -1.8 4,157,283 + oxyR b3961 0.84 -0.0 4,157,291 - oxyR b3961 0.85 -0.2 4,157,330 - oxyR b3961 0.89 -1.3 4,157,338 - oxyR b3961 0.90 +0.6 4,157,341 + +1.3 4,157,349 - -0.8 4,157,354 + +0.5
Or see this region's nucleotide sequence