Strain Fitness in Escherichia coli BW25113 around b4120

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmelA and melB are separated by 102 nucleotidesmelB and yjdF are separated by 138 nucleotidesyjdF and fumB are separated by 121 nucleotides b4119: melA - alpha-galactosidase, NAD(P)-binding (NCBI), at 4,339,934 to 4,341,289 melA b4120: melB - melibiose:sodium symporter (NCBI), at 4,341,392 to 4,342,813 melB b4121: yjdF - conserved inner membrane protein (NCBI), at 4,342,952 to 4,343,581 yjdF b4122: fumB - anaerobic class I fumarate hydratase (fumarase B) (NCBI), at 4,343,703 to 4,345,349 fumB Position (kb) 4341 4342 4343Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4340.413 kb on - strand, within melAat 4340.413 kb on - strand, within melAat 4340.430 kb on + strand, within melAat 4340.440 kb on + strand, within melAat 4340.452 kb on - strand, within melAat 4340.452 kb on - strand, within melAat 4340.457 kb on - strand, within melAat 4340.457 kb on - strand, within melAat 4340.746 kb on + strand, within melAat 4340.746 kb on + strand, within melAat 4340.746 kb on + strand, within melAat 4340.754 kb on - strand, within melAat 4340.765 kb on + strand, within melAat 4340.765 kb on + strand, within melAat 4340.799 kb on - strand, within melAat 4340.869 kb on + strand, within melAat 4340.917 kb on - strand, within melAat 4340.924 kb on + strand, within melAat 4340.934 kb on - strand, within melAat 4340.944 kb on - strand, within melAat 4340.944 kb on - strand, within melAat 4340.981 kb on - strand, within melAat 4341.084 kb on - strand, within melAat 4341.084 kb on - strand, within melAat 4341.111 kb on - strand, within melAat 4341.133 kb on - strand, within melAat 4341.176 kb on + strandat 4341.176 kb on - strandat 4341.211 kb on - strandat 4341.211 kb on - strandat 4341.230 kb on + strandat 4341.262 kb on - strandat 4341.293 kb on - strandat 4341.293 kb on - strandat 4341.296 kb on + strandat 4341.329 kb on + strandat 4341.380 kb on + strandat 4341.396 kb on - strandat 4341.464 kb on + strandat 4341.472 kb on - strandat 4341.477 kb on - strandat 4341.479 kb on + strandat 4341.484 kb on + strandat 4341.508 kb on + strandat 4341.508 kb on + strandat 4341.518 kb on - strandat 4341.518 kb on - strandat 4341.528 kb on + strandat 4341.538 kb on + strand, within melBat 4341.538 kb on + strand, within melBat 4341.641 kb on + strand, within melBat 4341.670 kb on + strand, within melBat 4341.688 kb on + strand, within melBat 4341.688 kb on + strand, within melBat 4341.696 kb on + strand, within melBat 4341.696 kb on + strand, within melBat 4341.704 kb on - strand, within melBat 4341.704 kb on - strand, within melBat 4341.724 kb on + strand, within melBat 4341.763 kb on - strand, within melBat 4341.786 kb on + strand, within melBat 4341.809 kb on - strand, within melBat 4341.939 kb on + strand, within melBat 4341.939 kb on + strand, within melBat 4341.939 kb on + strand, within melBat 4341.939 kb on + strand, within melBat 4341.951 kb on - strand, within melBat 4341.951 kb on - strand, within melBat 4341.975 kb on + strand, within melBat 4341.975 kb on + strand, within melBat 4341.979 kb on - strand, within melBat 4341.980 kb on + strand, within melBat 4341.981 kb on - strand, within melBat 4341.983 kb on - strand, within melBat 4341.985 kb on + strand, within melBat 4341.985 kb on + strand, within melBat 4341.990 kb on + strand, within melBat 4341.990 kb on + strand, within melBat 4342.014 kb on + strand, within melBat 4342.028 kb on + strand, within melBat 4342.039 kb on - strand, within melBat 4342.041 kb on + strand, within melBat 4342.041 kb on + strand, within melBat 4342.055 kb on - strand, within melBat 4342.055 kb on - strand, within melBat 4342.104 kb on - strand, within melBat 4342.179 kb on - strand, within melBat 4342.187 kb on + strand, within melBat 4342.200 kb on + strand, within melBat 4342.200 kb on + strand, within melBat 4342.202 kb on + strand, within melBat 4342.202 kb on + strand, within melBat 4342.215 kb on - strand, within melBat 4342.218 kb on - strand, within melBat 4342.220 kb on + strand, within melBat 4342.228 kb on - strand, within melBat 4342.228 kb on - strand, within melBat 4342.244 kb on - strand, within melBat 4342.261 kb on - strand, within melBat 4342.265 kb on - strand, within melBat 4342.283 kb on + strand, within melBat 4342.313 kb on - strand, within melBat 4342.313 kb on - strand, within melBat 4342.318 kb on - strand, within melBat 4342.331 kb on - strand, within melBat 4342.331 kb on - strand, within melBat 4342.333 kb on - strand, within melBat 4342.333 kb on - strand, within melBat 4342.357 kb on - strand, within melBat 4342.357 kb on - strand, within melBat 4342.359 kb on - strand, within melBat 4342.359 kb on - strand, within melBat 4342.369 kb on - strand, within melBat 4342.416 kb on + strand, within melBat 4342.416 kb on + strand, within melBat 4342.469 kb on - strand, within melBat 4342.469 kb on - strand, within melBat 4342.488 kb on - strand, within melBat 4342.492 kb on + strand, within melBat 4342.492 kb on + strand, within melBat 4342.492 kb on + strand, within melBat 4342.500 kb on - strand, within melBat 4342.500 kb on - strand, within melBat 4342.507 kb on + strand, within melBat 4342.509 kb on + strand, within melBat 4342.509 kb on + strand, within melBat 4342.510 kb on - strand, within melBat 4342.517 kb on - strand, within melBat 4342.517 kb on - strand, within melBat 4342.549 kb on + strand, within melBat 4342.579 kb on - strand, within melBat 4342.579 kb on - strand, within melBat 4342.587 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.597 kb on + strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.605 kb on - strand, within melBat 4342.624 kb on + strand, within melBat 4342.633 kb on - strand, within melBat 4342.633 kb on - strand, within melBat 4342.659 kb on + strand, within melBat 4342.662 kb on - strand, within melBat 4342.705 kb on - strandat 4342.718 kb on + strandat 4342.719 kb on - strandat 4342.719 kb on - strandat 4342.720 kb on - strandat 4342.773 kb on + strandat 4342.786 kb on - strandat 4342.786 kb on - strandat 4342.788 kb on + strandat 4342.797 kb on - strandat 4342.817 kb on + strandat 4342.831 kb on - strandat 4342.831 kb on - strandat 4342.913 kb on + strandat 4342.913 kb on + strandat 4342.937 kb on + strandat 4342.966 kb on + strandat 4343.050 kb on - strand, within yjdFat 4343.151 kb on + strand, within yjdFat 4343.169 kb on - strand, within yjdFat 4343.182 kb on - strand, within yjdFat 4343.182 kb on - strand, within yjdFat 4343.242 kb on + strand, within yjdFat 4343.268 kb on - strand, within yjdFat 4343.289 kb on + strand, within yjdFat 4343.292 kb on + strand, within yjdFat 4343.292 kb on + strand, within yjdFat 4343.299 kb on + strand, within yjdFat 4343.313 kb on - strand, within yjdFat 4343.313 kb on - strand, within yjdFat 4343.376 kb on - strand, within yjdFat 4343.399 kb on + strand, within yjdFat 4343.402 kb on - strand, within yjdFat 4343.516 kb on + strand, within yjdFat 4343.516 kb on + strand, within yjdFat 4343.527 kb on + strandat 4343.546 kb on + strandat 4343.547 kb on + strandat 4343.547 kb on + strandat 4343.579 kb on + strandat 4343.579 kb on + strandat 4343.666 kb on + strandat 4343.684 kb on - strandat 4343.700 kb on + strandat 4343.715 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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4,340,413 - melA b4119 0.35 -1.1
4,340,413 - melA b4119 0.35 +0.3
4,340,430 + melA b4119 0.37 -0.9
4,340,440 + melA b4119 0.37 +0.3
4,340,452 - melA b4119 0.38 -0.4
4,340,452 - melA b4119 0.38 -1.7
4,340,457 - melA b4119 0.39 +1.2
4,340,457 - melA b4119 0.39 +0.2
4,340,746 + melA b4119 0.60 +0.1
4,340,746 + melA b4119 0.60 +0.5
4,340,746 + melA b4119 0.60 -1.0
4,340,754 - melA b4119 0.60 -1.7
4,340,765 + melA b4119 0.61 -2.3
4,340,765 + melA b4119 0.61 -2.3
4,340,799 - melA b4119 0.64 +3.3
4,340,869 + melA b4119 0.69 +0.8
4,340,917 - melA b4119 0.72 -2.5
4,340,924 + melA b4119 0.73 +1.6
4,340,934 - melA b4119 0.74 +0.4
4,340,944 - melA b4119 0.74 +0.8
4,340,944 - melA b4119 0.74 -0.0
4,340,981 - melA b4119 0.77 +0.7
4,341,084 - melA b4119 0.85 +0.4
4,341,084 - melA b4119 0.85 -1.8
4,341,111 - melA b4119 0.87 +1.0
4,341,133 - melA b4119 0.88 -2.3
4,341,176 + +1.3
4,341,176 - +0.3
4,341,211 - +1.4
4,341,211 - +2.0
4,341,230 + +1.1
4,341,262 - -2.0
4,341,293 - -1.7
4,341,293 - -0.1
4,341,296 + -0.1
4,341,329 + +0.5
4,341,380 + -2.9
4,341,396 - -1.8
4,341,464 + +0.4
4,341,472 - +0.4
4,341,477 - -0.5
4,341,479 + +0.6
4,341,484 + -1.9
4,341,508 + -1.0
4,341,508 + -1.3
4,341,518 - -0.0
4,341,518 - +1.6
4,341,528 + +1.0
4,341,538 + melB b4120 0.10 +0.3
4,341,538 + melB b4120 0.10 +0.1
4,341,641 + melB b4120 0.18 -2.4
4,341,670 + melB b4120 0.20 +0.9
4,341,688 + melB b4120 0.21 +0.9
4,341,688 + melB b4120 0.21 +0.7
4,341,696 + melB b4120 0.21 -3.0
4,341,696 + melB b4120 0.21 -1.2
4,341,704 - melB b4120 0.22 -0.3
4,341,704 - melB b4120 0.22 -0.2
4,341,724 + melB b4120 0.23 +0.9
4,341,763 - melB b4120 0.26 -1.5
4,341,786 + melB b4120 0.28 -1.1
4,341,809 - melB b4120 0.29 +0.3
4,341,939 + melB b4120 0.38 -0.7
4,341,939 + melB b4120 0.38 +1.2
4,341,939 + melB b4120 0.38 +0.6
4,341,939 + melB b4120 0.38 +0.8
4,341,951 - melB b4120 0.39 -2.9
4,341,951 - melB b4120 0.39 +4.1
4,341,975 + melB b4120 0.41 +1.5
4,341,975 + melB b4120 0.41 -1.1
4,341,979 - melB b4120 0.41 -0.7
4,341,980 + melB b4120 0.41 -0.3
4,341,981 - melB b4120 0.41 +0.0
4,341,983 - melB b4120 0.42 +1.2
4,341,985 + melB b4120 0.42 +1.2
4,341,985 + melB b4120 0.42 +1.3
4,341,990 + melB b4120 0.42 -0.3
4,341,990 + melB b4120 0.42 -0.0
4,342,014 + melB b4120 0.44 +1.0
4,342,028 + melB b4120 0.45 +0.9
4,342,039 - melB b4120 0.45 -1.1
4,342,041 + melB b4120 0.46 +0.7
4,342,041 + melB b4120 0.46 +1.9
4,342,055 - melB b4120 0.47 +0.2
4,342,055 - melB b4120 0.47 +0.2
4,342,104 - melB b4120 0.50 -0.4
4,342,179 - melB b4120 0.55 -1.6
4,342,187 + melB b4120 0.56 -0.0
4,342,200 + melB b4120 0.57 -0.8
4,342,200 + melB b4120 0.57 +1.1
4,342,202 + melB b4120 0.57 -2.4
4,342,202 + melB b4120 0.57 +1.0
4,342,215 - melB b4120 0.58 +2.6
4,342,218 - melB b4120 0.58 +1.0
4,342,220 + melB b4120 0.58 +2.3
4,342,228 - melB b4120 0.59 -1.2
4,342,228 - melB b4120 0.59 +0.2
4,342,244 - melB b4120 0.60 -1.5
4,342,261 - melB b4120 0.61 -0.5
4,342,265 - melB b4120 0.61 -0.3
4,342,283 + melB b4120 0.63 -0.5
4,342,313 - melB b4120 0.65 -2.2
4,342,313 - melB b4120 0.65 +1.2
4,342,318 - melB b4120 0.65 +1.3
4,342,331 - melB b4120 0.66 +1.2
4,342,331 - melB b4120 0.66 -0.3
4,342,333 - melB b4120 0.66 +0.5
4,342,333 - melB b4120 0.66 +1.3
4,342,357 - melB b4120 0.68 +0.6
4,342,357 - melB b4120 0.68 +0.6
4,342,359 - melB b4120 0.68 +0.1
4,342,359 - melB b4120 0.68 -0.8
4,342,369 - melB b4120 0.69 -0.2
4,342,416 + melB b4120 0.72 -0.9
4,342,416 + melB b4120 0.72 +1.4
4,342,469 - melB b4120 0.76 +0.8
4,342,469 - melB b4120 0.76 +0.6
4,342,488 - melB b4120 0.77 +0.8
4,342,492 + melB b4120 0.77 +1.2
4,342,492 + melB b4120 0.77 +0.2
4,342,492 + melB b4120 0.77 -2.6
4,342,500 - melB b4120 0.78 -0.3
4,342,500 - melB b4120 0.78 -2.4
4,342,507 + melB b4120 0.78 +2.1
4,342,509 + melB b4120 0.79 -0.6
4,342,509 + melB b4120 0.79 +1.6
4,342,510 - melB b4120 0.79 +1.5
4,342,517 - melB b4120 0.79 +0.7
4,342,517 - melB b4120 0.79 +0.1
4,342,549 + melB b4120 0.81 -0.7
4,342,579 - melB b4120 0.83 +2.4
4,342,579 - melB b4120 0.83 +0.5
4,342,587 + melB b4120 0.84 +1.5
4,342,597 + melB b4120 0.85 +0.9
4,342,597 + melB b4120 0.85 -0.7
4,342,597 + melB b4120 0.85 +0.6
4,342,597 + melB b4120 0.85 +1.4
4,342,597 + melB b4120 0.85 +1.4
4,342,597 + melB b4120 0.85 -1.0
4,342,597 + melB b4120 0.85 +2.4
4,342,597 + melB b4120 0.85 +0.3
4,342,597 + melB b4120 0.85 +0.6
4,342,605 - melB b4120 0.85 -1.9
4,342,605 - melB b4120 0.85 -1.7
4,342,605 - melB b4120 0.85 -1.0
4,342,605 - melB b4120 0.85 +0.3
4,342,605 - melB b4120 0.85 -0.3
4,342,605 - melB b4120 0.85 +0.2
4,342,605 - melB b4120 0.85 +2.8
4,342,605 - melB b4120 0.85 +2.0
4,342,605 - melB b4120 0.85 -0.9
4,342,624 + melB b4120 0.87 +0.2
4,342,633 - melB b4120 0.87 -1.8
4,342,633 - melB b4120 0.87 +1.5
4,342,659 + melB b4120 0.89 +0.2
4,342,662 - melB b4120 0.89 +0.8
4,342,705 - -1.1
4,342,718 + +0.3
4,342,719 - -3.5
4,342,719 - +0.2
4,342,720 - -1.1
4,342,773 + +1.6
4,342,786 - -1.3
4,342,786 - -0.9
4,342,788 + +0.3
4,342,797 - -2.2
4,342,817 + -1.8
4,342,831 - -0.6
4,342,831 - -0.5
4,342,913 + +0.6
4,342,913 + -1.0
4,342,937 + -0.6
4,342,966 + -0.8
4,343,050 - yjdF b4121 0.16 +0.8
4,343,151 + yjdF b4121 0.32 +0.4
4,343,169 - yjdF b4121 0.34 +0.7
4,343,182 - yjdF b4121 0.37 +0.3
4,343,182 - yjdF b4121 0.37 +1.7
4,343,242 + yjdF b4121 0.46 +1.1
4,343,268 - yjdF b4121 0.50 +0.7
4,343,289 + yjdF b4121 0.53 -0.1
4,343,292 + yjdF b4121 0.54 +0.1
4,343,292 + yjdF b4121 0.54 +0.8
4,343,299 + yjdF b4121 0.55 +0.7
4,343,313 - yjdF b4121 0.57 +1.4
4,343,313 - yjdF b4121 0.57 +0.2
4,343,376 - yjdF b4121 0.67 -0.3
4,343,399 + yjdF b4121 0.71 -0.3
4,343,402 - yjdF b4121 0.71 -0.6
4,343,516 + yjdF b4121 0.90 +0.3
4,343,516 + yjdF b4121 0.90 -2.6
4,343,527 + +0.2
4,343,546 + -1.5
4,343,547 + -0.3
4,343,547 + -0.9
4,343,579 + +1.8
4,343,579 + +1.8
4,343,666 + -2.1
4,343,684 - +1.4
4,343,700 + -1.0
4,343,715 + -0.3

Or see this region's nucleotide sequence