Strain Fitness in Escherichia coli BW25113 around b3538

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
3,695,251	+	bcsE	b3536	0.49	+0.0
3,695,251	+	bcsE	b3536	0.49	+0.4
3,695,251	+	bcsE	b3536	0.49	+1.3
3,695,305	+	bcsE	b3536	0.52	+0.5
3,695,336	-	bcsE	b3536	0.54	-0.4
3,695,336	-	bcsE	b3536	0.54	-0.4
3,695,336	-	bcsE	b3536	0.54	+0.5
3,695,336	-	bcsE	b3536	0.54	-0.7
3,695,336	-	bcsE	b3536	0.54	+0.9
3,695,336	-	bcsE	b3536	0.54	+0.7
3,695,352	+	bcsE	b3536	0.55	+0.4
3,695,385	-	bcsE	b3536	0.58	-0.8
3,695,460	-	bcsE	b3536	0.62	+0.4
3,695,462	-	bcsE	b3536	0.62	-0.0
3,695,462	-	bcsE	b3536	0.62	+1.4
3,695,472	+	bcsE	b3536	0.63	+2.2
3,695,480	-	bcsE	b3536	0.64	+0.6
3,695,480	-	bcsE	b3536	0.64	+0.1
3,695,520	+	bcsE	b3536	0.66	+0.9
3,695,567	-	bcsE	b3536	0.69	-0.4
3,695,567	-	bcsE	b3536	0.69	-1.6
3,695,581	+	bcsE	b3536	0.70	-0.5
3,695,582	-	bcsE	b3536	0.70	+0.6
3,695,583	+	bcsE	b3536	0.70	+0.8
3,695,591	+	bcsE	b3536	0.71	+0.1
3,695,596	+	bcsE	b3536	0.71	-0.7
3,695,632	+	bcsE	b3536	0.73	-0.1
3,695,637	-	bcsE	b3536	0.74	+0.7
3,695,637	-	bcsE	b3536	0.74	+0.0
3,695,638	-	bcsE	b3536	0.74	+2.1
3,695,644	+	bcsE	b3536	0.74	-0.1
3,695,644	+	bcsE	b3536	0.74	+2.9
3,695,700	+	bcsE	b3536	0.78	-1.1
3,695,757	+	bcsE	b3536	0.81	-1.2
3,695,814	+	bcsE	b3536	0.85	+0.1
3,695,871	-	bcsE	b3536	0.88	+0.6
3,695,894	+	bcsE	b3536	0.90	+2.5
3,695,902	-				+0.5
3,695,902	-				-0.0
3,695,902	-				-2.8
3,695,926	+				+2.0
3,695,928	-				+0.9
3,695,938	+				+1.0
3,695,952	+				+2.4
3,695,952	+				-1.2
3,695,965	+				-0.8
3,695,985	+				-1.7
3,695,994	+				-2.4
3,696,022	+				-1.3
3,696,022	+				+0.8
3,696,032	+				+0.3
3,696,124	-	yhjT	b3537	0.39	-1.3
3,696,124	-	yhjT	b3537	0.39	+1.4
3,696,131	+	yhjT	b3537	0.43	+0.8
3,696,131	+	yhjT	b3537	0.43	+0.4
3,696,199	+	yhjT	b3537	0.78	+0.9
3,696,205	-	yhjT	b3537	0.81	+0.5
3,696,268	+				-0.3
3,696,292	-				+0.4
3,696,338	+				+0.5
3,696,338	+				-0.5
3,696,346	-				-0.6
3,696,346	-				+2.2
3,696,352	+				-0.7
3,696,416	+	bcsG	b3538	0.11	+0.8
3,696,416	+	bcsG	b3538	0.11	+0.1
3,696,416	+	bcsG	b3538	0.11	-0.7
3,696,431	-	bcsG	b3538	0.12	-0.1
3,696,445	-	bcsG	b3538	0.12	+0.4
3,696,450	-	bcsG	b3538	0.13	+0.6
3,696,473	-	bcsG	b3538	0.14	-0.0
3,696,473	-	bcsG	b3538	0.14	-0.6
3,696,473	-	bcsG	b3538	0.14	-1.1
3,696,504	-	bcsG	b3538	0.16	-1.3
3,696,519	+	bcsG	b3538	0.17	-0.4
3,696,544	-	bcsG	b3538	0.18	+0.0
3,696,581	-	bcsG	b3538	0.20	+0.7
3,696,630	-	bcsG	b3538	0.23	+0.0
3,696,677	-	bcsG	b3538	0.26	+2.9
3,696,677	-	bcsG	b3538	0.26	-0.1
3,696,681	-	bcsG	b3538	0.26	-0.6
3,696,714	+	bcsG	b3538	0.28	+2.1
3,696,753	-	bcsG	b3538	0.31	-0.5
3,696,753	-	bcsG	b3538	0.31	-0.2
3,696,771	-	bcsG	b3538	0.32	+1.5
3,696,771	-	bcsG	b3538	0.32	-2.8
3,696,804	+	bcsG	b3538	0.34	+2.2
3,696,804	+	bcsG	b3538	0.34	+4.2
3,696,829	-	bcsG	b3538	0.35	+0.7
3,696,833	+	bcsG	b3538	0.35	+0.9
3,696,833	+	bcsG	b3538	0.35	-0.1
3,696,851	+	bcsG	b3538	0.37	-0.0
3,696,925	+	bcsG	b3538	0.41	+2.5
3,696,950	+	bcsG	b3538	0.42	+0.9
3,696,980	+				+1.1
3,696,989	-	bcsG	b3538	0.45	+0.5
3,697,005	-	bcsG	b3538	0.46	+2.6
3,697,005	-	bcsG	b3538	0.46	-1.2
3,697,052	+	bcsG	b3538	0.49	-0.6
3,697,061	+	bcsG	b3538	0.49	+0.0
3,697,062	+	bcsG	b3538	0.49	+0.6
3,697,067	-	bcsG	b3538	0.49	-0.3
3,697,069	-	bcsG	b3538	0.50	+1.7
3,697,072	+	bcsG	b3538	0.50	-0.4
3,697,084	+	bcsG	b3538	0.50	+0.2
3,697,116	+	bcsG	b3538	0.52	+0.8
3,697,120	+	bcsG	b3538	0.53	-1.2
3,697,128	-	bcsG	b3538	0.53	-0.0
3,697,138	-	bcsG	b3538	0.54	+2.6
3,697,326	+	bcsG	b3538	0.65	+2.2
3,697,336	+	bcsG	b3538	0.65	+0.9
3,697,339	+	bcsG	b3538	0.66	-0.9
3,697,339	+	bcsG	b3538	0.66	+1.7
3,697,344	+	bcsG	b3538	0.66	+1.7
3,697,352	-	bcsG	b3538	0.66	+0.4
3,697,395	-	bcsG	b3538	0.69	-0.9
3,697,395	-	bcsG	b3538	0.69	+0.3
3,697,401	-	bcsG	b3538	0.69	+0.9
3,697,401	-	bcsG	b3538	0.69	+2.8
3,697,408	+	bcsG	b3538	0.70	+0.4
3,697,419	-	bcsG	b3538	0.70	+2.1
3,697,433	-	bcsG	b3538	0.71	-0.1
3,697,441	-	bcsG	b3538	0.72	-1.7
3,697,441	-	bcsG	b3538	0.72	+1.3
3,697,547	-	bcsG	b3538	0.78	+0.7
3,697,560	+	bcsG	b3538	0.79	-0.9
3,697,568	-	bcsG	b3538	0.79	+1.2
3,697,570	-	bcsG	b3538	0.79	+3.4
3,697,607	+	bcsG	b3538	0.82	-0.8
3,697,607	+	bcsG	b3538	0.82	-4.2
3,697,615	+	bcsG	b3538	0.82	+1.3
3,697,615	+	bcsG	b3538	0.82	+1.3
3,697,677	+	bcsG	b3538	0.86	+1.1
3,697,736	+	bcsG	b3538	0.89	-1.1
3,697,861	+				+1.6
3,697,886	-				-3.1
3,697,886	-				+0.9
3,697,945	+				+2.6
3,697,945	+				+0.5
3,698,018	+	ldrD	b4453	0.14	-0.9
3,698,052	+	ldrD	b4453	0.45	+2.6
3,698,055	-	ldrD	b4453	0.48	-1.1
3,698,103	-				+1.6
3,698,103	-				+2.6
3,698,107	+				-0.2
3,698,158	+				+0.0
3,698,158	+				+0.2
3,698,158	+				-0.4
3,698,158	+				-1.1
3,698,165	+				+0.6
3,698,165	+				+0.5
3,698,166	-	rdlD	b4454	0.11	+0.8
3,698,178	-	rdlD	b4454	0.29	+0.0
3,698,185	+	rdlD	b4454	0.39	-0.8
3,698,190	+	rdlD	b4454	0.47	+0.3
3,698,195	+	rdlD	b4454	0.55	-1.1
3,698,195	+	rdlD	b4454	0.55	-0.1
3,698,195	+	rdlD	b4454	0.55	-3.3
3,698,195	+	rdlD	b4454	0.55	+0.1
3,698,257	-				+0.9
3,698,260	+				+0.6
3,698,277	+				-1.7
3,698,318	+				-1.5
3,698,333	+				-0.6
3,698,333	+				-1.0
3,698,340	+				+1.0
3,698,407	-				-2.6
3,698,410	+				+1.8
3,698,410	+				-1.8
3,698,410	+				+0.3
3,698,410	+				-0.1
3,698,411	+				-0.6
3,698,418	-				-1.4
3,698,418	-				-3.0
3,698,418	-				-4.9
3,698,429	-				-0.9
3,698,429	-				-1.7
3,698,429	-				+0.1
3,698,452	+				-1.0
3,698,452	-				-0.0
3,698,473	+				+0.0
3,698,474	-				-0.3
3,698,474	-				+0.8
3,698,487	+				+1.2
3,698,487	+				+1.3
3,698,501	+				-0.5
3,698,501	+				+0.7
3,698,503	+				-2.9
3,698,503	+				-1.1
3,698,507	-				+0.8
3,698,519	-				+1.0
3,698,533	-				-0.8
3,698,533	-				-2.0
3,698,533	-				+0.1
3,698,589	-				+0.0
3,698,591	+				+1.1
3,698,591	+				+3.7
3,698,593	+				+0.9
3,698,607	-				-0.5
3,698,612	-				-1.7
3,698,632	+				+2.6
3,698,632	+				+0.5
3,698,634	+				+3.8
3,698,640	+				+0.2
3,698,642	-				+0.6
3,698,660	+				+0.5
3,698,660	+				-0.4
3,698,667	+				+2.2
3,698,681	+				-0.6
3,698,681	+				-0.7
3,698,683	+				-0.8
3,698,683	+				+0.2
3,698,689	+				+0.3
3,698,690	-				+0.9
3,698,691	+				+2.3
3,698,691	+				-0.8
3,698,693	+				-1.3
3,698,694	-				-0.8
3,698,696	-				+1.2
3,698,696	-				-2.0
3,698,699	-				-1.5
3,698,699	-				+1.1
3,698,699	-				-0.1
3,698,701	-				-1.6
3,698,715	-	yhjV	b3539	0.10	+0.7
3,698,755	-	yhjV	b3539	0.13	-0.6
3,698,760	+	yhjV	b3539	0.14	-1.3
3,698,760	-	yhjV	b3539	0.14	+0.2
3,698,760	-	yhjV	b3539	0.14	+0.2
3,698,761	-	yhjV	b3539	0.14	+0.6
3,698,761	-	yhjV	b3539	0.14	-1.8
3,698,765	+	yhjV	b3539	0.14	+0.1
3,698,765	+	yhjV	b3539	0.14	-0.1
3,698,765	+	yhjV	b3539	0.14	-0.1
3,698,765	+	yhjV	b3539	0.14	-0.5
3,698,765	+	yhjV	b3539	0.14	+0.2
3,698,773	-	yhjV	b3539	0.15	-0.2
3,698,773	-	yhjV	b3539	0.15	-0.0
3,698,773	-	yhjV	b3539	0.15	+1.5
3,698,773	-	yhjV	b3539	0.15	-0.2
3,698,775	+	yhjV	b3539	0.15	+0.5
3,698,783	+	yhjV	b3539	0.15	+0.3
3,698,783	-	yhjV	b3539	0.15	+1.1
3,698,783	-	yhjV	b3539	0.15	+1.2
3,698,783	-	yhjV	b3539	0.15	-1.2
3,698,822	+	yhjV	b3539	0.19	-0.7
3,698,847	+	yhjV	b3539	0.21	+0.2
3,698,850	-	yhjV	b3539	0.21	-0.5
3,698,850	-	yhjV	b3539	0.21	+0.1
3,698,856	-	yhjV	b3539	0.21	-0.1
3,698,909	-	yhjV	b3539	0.25	+3.2
3,698,909	-	yhjV	b3539	0.25	-2.6