Strain Fitness in Escherichia coli BW25113 around b1941

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliG and fliH overlap by 8 nucleotidesfliH and fliI overlap by 1 nucleotidesfliI and fliJ are separated by 18 nucleotidesfliJ and fliK overlap by 4 nucleotides b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899 fliG b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578 fliH b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951 fliI b1942: fliJ - flagellar biosynthesis chaperone (NCBI), at 2,015,970 to 2,016,413 fliJ b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537 fliK Position (kb) 2014 2015 2016Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2014.036 kb on + strand, within fliHat 2014.099 kb on - strand, within fliHat 2014.120 kb on + strand, within fliHat 2014.214 kb on + strand, within fliHat 2014.240 kb on - strand, within fliHat 2014.246 kb on + strand, within fliHat 2014.318 kb on - strand, within fliHat 2014.336 kb on + strand, within fliHat 2014.404 kb on - strand, within fliHat 2014.562 kb on + strandat 2014.562 kb on + strandat 2014.829 kb on + strand, within fliIat 2014.829 kb on + strand, within fliIat 2014.917 kb on - strand, within fliIat 2014.920 kb on + strand, within fliIat 2014.920 kb on + strand, within fliIat 2015.057 kb on - strand, within fliIat 2015.057 kb on - strandat 2015.259 kb on - strand, within fliIat 2015.261 kb on - strand, within fliIat 2015.261 kb on - strand, within fliIat 2015.499 kb on - strand, within fliIat 2015.549 kb on + strand, within fliIat 2015.549 kb on + strand, within fliIat 2015.560 kb on + strand, within fliIat 2015.631 kb on + strand, within fliIat 2015.685 kb on - strand, within fliIat 2015.685 kb on - strand, within fliIat 2015.701 kb on + strandat 2015.701 kb on + strand, within fliIat 2015.847 kb on - strandat 2015.847 kb on - strandat 2016.216 kb on - strand, within fliJat 2016.216 kb on - strand, within fliJat 2016.658 kb on - strand, within fliKat 2016.927 kb on - strand, within fliK

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,014,036 + fliH b1940 0.21 -1.2
2,014,099 - fliH b1940 0.30 +1.8
2,014,120 + fliH b1940 0.33 +0.2
2,014,214 + fliH b1940 0.47 -1.2
2,014,240 - fliH b1940 0.51 -1.6
2,014,246 + fliH b1940 0.52 -0.8
2,014,318 - fliH b1940 0.62 +0.9
2,014,336 + fliH b1940 0.65 -3.0
2,014,404 - fliH b1940 0.75 -2.4
2,014,562 + -1.8
2,014,562 + +2.2
2,014,829 + fliI b1941 0.18 -0.9
2,014,829 + fliI b1941 0.18 +0.5
2,014,917 - fliI b1941 0.25 -0.6
2,014,920 + fliI b1941 0.25 -1.3
2,014,920 + fliI b1941 0.25 -3.0
2,015,057 - fliI b1941 0.35 -2.6
2,015,057 - +0.2
2,015,259 - fliI b1941 0.50 -1.5
2,015,261 - fliI b1941 0.50 -2.5
2,015,261 - fliI b1941 0.50 -1.3
2,015,499 - fliI b1941 0.67 -0.7
2,015,549 + fliI b1941 0.71 +2.1
2,015,549 + fliI b1941 0.71 -2.0
2,015,560 + fliI b1941 0.71 +0.1
2,015,631 + fliI b1941 0.77 +0.3
2,015,685 - fliI b1941 0.81 -1.7
2,015,685 - fliI b1941 0.81 -0.8
2,015,701 + +3.7
2,015,701 + fliI b1941 0.82 -2.0
2,015,847 - +1.0
2,015,847 - -2.0
2,016,216 - fliJ b1942 0.55 -0.4
2,016,216 - fliJ b1942 0.55 -0.2
2,016,658 - fliK b1943 0.22 -2.0
2,016,927 - fliK b1943 0.46 -1.4

Or see this region's nucleotide sequence