Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspA and pspB are separated by 53 nucleotides pspB and pspC overlap by 1 nucleotides pspC and pspD are separated by 8 nucleotides pspD and pspE are separated by 74 nucleotides pspE and ycjM are separated by 212 nucleotides
b1304: pspA - regulatory protein for phage-shock-protein operon (NCBI), at 1,366,103 to 1,366,771
pspA
b1305: pspB - phage shock protein B (NCBI), at 1,366,825 to 1,367,049
pspB
b1306: pspC - DNA-binding transcriptional activator (NCBI), at 1,367,049 to 1,367,408
pspC
b1307: pspD - peripheral inner membrane phage-shock protein (NCBI), at 1,367,417 to 1,367,638
pspD
b1308: pspE - thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI), at 1,367,713 to 1,368,027
pspE
b1309: ycjM - putative polysaccharide hydrolase (VIMSS), at 1,368,240 to 1,369,919
ycjM
Position (kb) 1367
1368
1369 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1366.818 kb on - strand at 1366.840 kb on - strand at 1366.896 kb on + strand, within pspB at 1366.931 kb on + strand, within pspB at 1366.945 kb on - strand, within pspB at 1367.086 kb on + strand, within pspC at 1367.105 kb on - strand, within pspC at 1367.191 kb on + strand, within pspC at 1367.191 kb on + strand, within pspC at 1367.384 kb on + strand at 1367.424 kb on + strand at 1367.424 kb on + strand at 1367.427 kb on + strand at 1367.591 kb on + strand, within pspD at 1367.631 kb on - strand at 1367.631 kb on - strand at 1367.795 kb on + strand, within pspE at 1367.795 kb on + strand, within pspE at 1367.813 kb on - strand, within pspE at 1367.842 kb on + strand, within pspE at 1367.852 kb on + strand, within pspE at 1367.860 kb on - strand, within pspE at 1367.932 kb on + strand, within pspE at 1367.941 kb on + strand, within pspE at 1367.990 kb on + strand, within pspE at 1367.991 kb on + strand, within pspE at 1367.991 kb on + strand at 1368.049 kb on - strand at 1368.065 kb on - strand at 1368.090 kb on + strand at 1368.156 kb on + strand at 1368.323 kb on + strand at 1368.323 kb on + strand at 1368.323 kb on + strand at 1368.348 kb on + strand at 1368.432 kb on + strand, within ycjM at 1368.492 kb on + strand, within ycjM at 1368.516 kb on - strand, within ycjM at 1368.517 kb on + strand, within ycjM at 1368.573 kb on + strand, within ycjM at 1368.583 kb on - strand, within ycjM at 1368.594 kb on - strand, within ycjM at 1368.634 kb on + strand, within ycjM at 1368.736 kb on - strand, within ycjM at 1368.739 kb on - strand, within ycjM at 1368.739 kb on - strand, within ycjM at 1368.766 kb on - strand, within ycjM at 1368.766 kb on - strand, within ycjM at 1368.814 kb on - strand, within ycjM at 1368.822 kb on - strand, within ycjM at 1368.858 kb on - strand, within ycjM at 1368.858 kb on - strand, within ycjM at 1368.880 kb on - strand, within ycjM at 1368.926 kb on - strand, within ycjM at 1368.928 kb on - strand, within ycjM
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,366,818 - +0.3 1,366,840 - +0.5 1,366,896 + pspB b1305 0.32 -0.0 1,366,931 + pspB b1305 0.47 -1.0 1,366,945 - pspB b1305 0.53 -0.6 1,367,086 + pspC b1306 0.10 +0.6 1,367,105 - pspC b1306 0.16 -0.2 1,367,191 + pspC b1306 0.39 -1.0 1,367,191 + pspC b1306 0.39 +2.6 1,367,384 + -2.3 1,367,424 + +0.5 1,367,424 + +1.2 1,367,427 + +1.5 1,367,591 + pspD b1307 0.78 -1.3 1,367,631 - +0.6 1,367,631 - +1.9 1,367,795 + pspE b1308 0.26 -1.3 1,367,795 + pspE b1308 0.26 -0.9 1,367,813 - pspE b1308 0.32 +0.6 1,367,842 + pspE b1308 0.41 +3.4 1,367,852 + pspE b1308 0.44 -0.0 1,367,860 - pspE b1308 0.47 +1.3 1,367,932 + pspE b1308 0.70 +1.9 1,367,941 + pspE b1308 0.72 +0.3 1,367,990 + pspE b1308 0.88 +1.1 1,367,991 + pspE b1308 0.88 -1.0 1,367,991 + -0.2 1,368,049 - -0.2 1,368,065 - -0.3 1,368,090 + -3.1 1,368,156 + +0.5 1,368,323 + -0.1 1,368,323 + +0.7 1,368,323 + -0.8 1,368,348 + +1.1 1,368,432 + ycjM b1309 0.11 -0.7 1,368,492 + ycjM b1309 0.15 -3.1 1,368,516 - ycjM b1309 0.16 -2.6 1,368,517 + ycjM b1309 0.16 -1.2 1,368,573 + ycjM b1309 0.20 -0.6 1,368,583 - ycjM b1309 0.20 -3.1 1,368,594 - ycjM b1309 0.21 -2.6 1,368,634 + ycjM b1309 0.23 -2.3 1,368,736 - ycjM b1309 0.30 -0.4 1,368,739 - ycjM b1309 0.30 -2.6 1,368,739 - ycjM b1309 0.30 +0.9 1,368,766 - ycjM b1309 0.31 +1.5 1,368,766 - ycjM b1309 0.31 -0.5 1,368,814 - ycjM b1309 0.34 +0.2 1,368,822 - ycjM b1309 0.35 +1.6 1,368,858 - ycjM b1309 0.37 +1.2 1,368,858 - ycjM b1309 0.37 -0.6 1,368,880 - ycjM b1309 0.38 +0.4 1,368,926 - ycjM b1309 0.41 +0.7 1,368,928 - ycjM b1309 0.41 -0.0
Or see this region's nucleotide sequence