Strain Fitness in Escherichia coli BW25113 around b1076

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgC and flgD are separated by 11 nucleotidesflgD and flgE are separated by 24 nucleotidesflgE and flgF are separated by 19 nucleotidesflgF and flgG are separated by 171 nucleotides b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065 flgC b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772 flgD b1076: flgE - flagellar hook protein E (NCBI), at 1,131,797 to 1,133,005 flgE b1077: flgF - flagellar component of cell-proximal portion of basal-body rod (NCBI), at 1,133,025 to 1,133,780 flgF b1078: flgG - flagellar component of cell-distal portion of basal-body rod (NCBI), at 1,133,952 to 1,134,734 flgG Position (kb) 1131 1132 1133 1134Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1131.014 kb on + strand, within flgCat 1131.014 kb on + strand, within flgCat 1131.054 kb on - strandat 1131.111 kb on + strandat 1131.325 kb on + strand, within flgDat 1131.362 kb on - strand, within flgDat 1131.362 kb on - strand, within flgDat 1131.586 kb on + strand, within flgDat 1131.594 kb on - strand, within flgDat 1131.794 kb on + strandat 1131.794 kb on + strandat 1131.846 kb on - strandat 1131.996 kb on - strand, within flgEat 1131.996 kb on - strand, within flgEat 1132.002 kb on - strand, within flgEat 1132.010 kb on + strand, within flgEat 1132.176 kb on - strand, within flgEat 1132.283 kb on - strand, within flgEat 1132.366 kb on + strand, within flgEat 1132.366 kb on + strand, within flgEat 1132.384 kb on + strand, within flgEat 1132.423 kb on + strand, within flgEat 1132.463 kb on - strand, within flgEat 1132.463 kb on - strand, within flgEat 1132.510 kb on - strand, within flgEat 1132.551 kb on + strand, within flgEat 1132.559 kb on - strand, within flgEat 1132.559 kb on - strand, within flgEat 1132.574 kb on - strand, within flgEat 1132.584 kb on - strand, within flgEat 1132.605 kb on - strand, within flgEat 1132.625 kb on - strand, within flgEat 1132.625 kb on - strand, within flgEat 1132.694 kb on - strand, within flgEat 1132.979 kb on - strandat 1132.986 kb on - strandat 1132.998 kb on - strandat 1132.998 kb on - strandat 1133.002 kb on + strandat 1133.037 kb on + strandat 1133.162 kb on - strand, within flgFat 1133.162 kb on - strand, within flgFat 1133.215 kb on - strand, within flgFat 1133.251 kb on - strand, within flgFat 1133.251 kb on - strand, within flgFat 1133.286 kb on - strand, within flgFat 1133.318 kb on - strand, within flgFat 1133.324 kb on - strand, within flgFat 1133.336 kb on + strand, within flgFat 1133.367 kb on + strand, within flgFat 1133.389 kb on - strand, within flgFat 1133.417 kb on - strand, within flgFat 1133.467 kb on - strand, within flgFat 1133.477 kb on + strand, within flgFat 1133.610 kb on + strand, within flgFat 1133.618 kb on - strand, within flgFat 1133.833 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,131,014 + flgC b1074 0.87 -2.8
1,131,014 + flgC b1074 0.87 -0.8
1,131,054 - -1.3
1,131,111 + -0.4
1,131,325 + flgD b1075 0.36 -1.3
1,131,362 - flgD b1075 0.41 -0.8
1,131,362 - flgD b1075 0.41 -1.5
1,131,586 + flgD b1075 0.73 -2.7
1,131,594 - flgD b1075 0.74 +0.1
1,131,794 + -3.0
1,131,794 + -1.8
1,131,846 - -2.1
1,131,996 - flgE b1076 0.16 +1.4
1,131,996 - flgE b1076 0.16 -0.9
1,132,002 - flgE b1076 0.17 -0.0
1,132,010 + flgE b1076 0.18 +1.2
1,132,176 - flgE b1076 0.31 -3.9
1,132,283 - flgE b1076 0.40 +0.7
1,132,366 + flgE b1076 0.47 -2.3
1,132,366 + flgE b1076 0.47 +0.0
1,132,384 + flgE b1076 0.49 -1.2
1,132,423 + flgE b1076 0.52 -1.2
1,132,463 - flgE b1076 0.55 -1.8
1,132,463 - flgE b1076 0.55 -1.3
1,132,510 - flgE b1076 0.59 -1.1
1,132,551 + flgE b1076 0.62 -1.4
1,132,559 - flgE b1076 0.63 -0.6
1,132,559 - flgE b1076 0.63 -2.7
1,132,574 - flgE b1076 0.64 +0.1
1,132,584 - flgE b1076 0.65 +1.0
1,132,605 - flgE b1076 0.67 -1.3
1,132,625 - flgE b1076 0.68 -2.4
1,132,625 - flgE b1076 0.68 +0.9
1,132,694 - flgE b1076 0.74 +0.6
1,132,979 - +0.2
1,132,986 - -0.2
1,132,998 - -1.3
1,132,998 - -1.7
1,133,002 + +1.0
1,133,037 + -1.9
1,133,162 - flgF b1077 0.18 +0.5
1,133,162 - flgF b1077 0.18 -0.8
1,133,215 - flgF b1077 0.25 -0.9
1,133,251 - flgF b1077 0.30 -0.6
1,133,251 - flgF b1077 0.30 -1.8
1,133,286 - flgF b1077 0.35 -1.7
1,133,318 - flgF b1077 0.39 +1.9
1,133,324 - flgF b1077 0.40 +0.8
1,133,336 + flgF b1077 0.41 -2.3
1,133,367 + flgF b1077 0.45 -2.4
1,133,389 - flgF b1077 0.48 -2.7
1,133,417 - flgF b1077 0.52 +0.1
1,133,467 - flgF b1077 0.58 -0.3
1,133,477 + flgF b1077 0.60 -1.9
1,133,610 + flgF b1077 0.77 +2.0
1,133,618 - flgF b1077 0.78 -0.3
1,133,833 + -1.4

Or see this region's nucleotide sequence