Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdL and ybdM are separated by 0 nucleotides ybdM and ybdN overlap by 28 nucleotides ybdN and ybdO are separated by 146 nucleotides
b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969
ybdL
b0601: ybdM - hypothetical protein (NCBI), at 633,970 to 634,599
ybdM
b0602: ybdN - hypothetical protein (NCBI), at 634,572 to 635,792
ybdN
b0603: ybdO - predicted DNA-binding transcriptional regulator (NCBI), at 635,939 to 636,841
ybdO
Position (kb) 634
635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 633.572 kb on + strand, within ybdL at 633.587 kb on + strand, within ybdL at 633.597 kb on - strand, within ybdL at 633.717 kb on - strand, within ybdL at 633.825 kb on + strand, within ybdL at 633.853 kb on - strand, within ybdL at 633.970 kb on + strand at 634.194 kb on + strand, within ybdM at 634.206 kb on + strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.309 kb on + strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.337 kb on - strand, within ybdM at 634.464 kb on - strand, within ybdM at 634.477 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.593 kb on + strand at 634.672 kb on + strand at 634.687 kb on + strand at 634.720 kb on - strand, within ybdN at 634.963 kb on + strand, within ybdN at 634.977 kb on + strand, within ybdN at 635.018 kb on + strand, within ybdN at 635.018 kb on + strand, within ybdN at 635.039 kb on - strand, within ybdN at 635.132 kb on + strand, within ybdN at 635.140 kb on - strand, within ybdN at 635.140 kb on - strand, within ybdN at 635.298 kb on - strand, within ybdN at 635.431 kb on - strand, within ybdN at 635.437 kb on - strand, within ybdN at 635.457 kb on - strand, within ybdN at 635.475 kb on + strand, within ybdN at 635.500 kb on + strand, within ybdN at 635.505 kb on + strand, within ybdN at 635.505 kb on + strand, within ybdN at 635.542 kb on + strand, within ybdN at 635.545 kb on - strand, within ybdN at 635.545 kb on - strand, within ybdN at 635.601 kb on + strand, within ybdN at 635.604 kb on - strand, within ybdN at 635.700 kb on + strand at 635.700 kb on + strand at 635.705 kb on + strand at 635.705 kb on + strand at 635.709 kb on + strand at 635.709 kb on + strand at 635.709 kb on + strand at 635.709 kb on + strand at 635.709 kb on - strand at 635.711 kb on + strand at 635.711 kb on + strand at 635.715 kb on + strand at 635.717 kb on - strand at 635.723 kb on + strand at 635.723 kb on + strand at 635.723 kb on + strand at 635.730 kb on + strand at 635.730 kb on + strand at 635.731 kb on - strand at 635.734 kb on + strand at 635.734 kb on + strand at 635.734 kb on + strand at 635.743 kb on + strand at 635.743 kb on + strand at 635.792 kb on + strand at 635.792 kb on + strand at 635.804 kb on + strand at 635.808 kb on + strand at 635.810 kb on + strand at 635.812 kb on + strand at 635.812 kb on + strand at 635.813 kb on + strand at 635.813 kb on + strand at 635.813 kb on + strand at 635.820 kb on - strand at 635.826 kb on + strand at 635.851 kb on + strand at 635.861 kb on + strand at 635.861 kb on + strand at 636.350 kb on - strand, within ybdO at 636.360 kb on - strand, within ybdO at 636.500 kb on + strand, within ybdO
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 633,572 + ybdL b0600 0.66 +2.6 633,587 + ybdL b0600 0.67 -0.3 633,597 - ybdL b0600 0.68 -0.0 633,717 - ybdL b0600 0.78 -0.2 633,825 + ybdL b0600 0.88 +2.8 633,853 - ybdL b0600 0.90 +1.4 633,970 + +2.0 634,194 + ybdM b0601 0.36 +0.7 634,206 + ybdM b0601 0.37 -0.8 634,214 - ybdM b0601 0.39 +0.9 634,214 - ybdM b0601 0.39 -0.4 634,214 - ybdM b0601 0.39 -1.5 634,214 - ybdM b0601 0.39 +0.1 634,214 - ybdM b0601 0.39 -1.2 634,309 + ybdM b0601 0.54 -0.2 634,335 - ybdM b0601 0.58 +0.4 634,335 - ybdM b0601 0.58 +0.2 634,337 - ybdM b0601 0.58 +0.6 634,464 - ybdM b0601 0.78 +1.3 634,477 - ybdM b0601 0.80 +0.7 634,486 - ybdM b0601 0.82 -0.0 634,486 - ybdM b0601 0.82 +1.1 634,593 + -0.1 634,672 + -0.0 634,687 + -1.8 634,720 - ybdN b0602 0.12 -0.0 634,963 + ybdN b0602 0.32 +0.5 634,977 + ybdN b0602 0.33 -0.7 635,018 + ybdN b0602 0.37 -0.8 635,018 + ybdN b0602 0.37 -0.2 635,039 - ybdN b0602 0.38 +1.1 635,132 + ybdN b0602 0.46 +0.4 635,140 - ybdN b0602 0.47 +0.1 635,140 - ybdN b0602 0.47 +0.2 635,298 - ybdN b0602 0.59 +0.7 635,431 - ybdN b0602 0.70 +0.3 635,437 - ybdN b0602 0.71 +2.6 635,457 - ybdN b0602 0.72 -0.5 635,475 + ybdN b0602 0.74 +1.0 635,500 + ybdN b0602 0.76 +1.9 635,505 + ybdN b0602 0.76 -0.7 635,505 + ybdN b0602 0.76 -1.0 635,542 + ybdN b0602 0.79 -0.1 635,545 - ybdN b0602 0.80 +0.0 635,545 - ybdN b0602 0.80 -0.1 635,601 + ybdN b0602 0.84 +0.5 635,604 - ybdN b0602 0.85 -0.4 635,700 + +0.5 635,700 + +2.8 635,705 + +0.9 635,705 + -0.4 635,709 + -0.5 635,709 + -0.4 635,709 + +1.7 635,709 + +0.9 635,709 - -0.1 635,711 + -1.9 635,711 + +0.8 635,715 + +0.9 635,717 - -0.5 635,723 + +0.3 635,723 + +0.1 635,723 + -0.3 635,730 + -0.6 635,730 + +0.4 635,731 - -1.8 635,734 + +1.9 635,734 + -0.7 635,734 + +0.7 635,743 + -0.4 635,743 + +0.3 635,792 + +0.4 635,792 + +0.3 635,804 + -1.2 635,808 + -1.0 635,810 + -3.3 635,812 + +0.1 635,812 + +0.7 635,813 + -0.4 635,813 + +1.8 635,813 + -0.4 635,820 - -3.0 635,826 + -2.5 635,851 + -2.8 635,861 + -1.9 635,861 + -3.4 636,350 - ybdO b0603 0.46 -0.3 636,360 - ybdO b0603 0.47 -3.1 636,500 + ybdO b0603 0.62 -3.1
Or see this region's nucleotide sequence