Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahK and yahL are separated by 242 nucleotides yahL and yahM are separated by 412 nucleotides yahM and yahN are separated by 16 nucleotides
b0325: yahK - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 342,108 to 343,157
yahK
b0326: yahL - hypothetical protein (NCBI), at 343,400 to 344,215
yahL
b0327: yahM - orf, hypothetical protein (VIMSS), at 344,628 to 344,873
yahM
b0328: yahN - neutral amino-acid efflux system (NCBI), at 344,890 to 345,561
yahN
Position (kb) 343
344
345 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 342.422 kb on + strand, within yahK at 342.445 kb on + strand, within yahK at 342.488 kb on - strand, within yahK at 342.493 kb on + strand, within yahK at 342.510 kb on + strand, within yahK at 342.525 kb on + strand, within yahK at 342.525 kb on + strand, within yahK at 342.714 kb on + strand, within yahK at 342.714 kb on + strand, within yahK at 342.906 kb on - strand, within yahK at 342.979 kb on + strand, within yahK at 342.979 kb on + strand, within yahK at 342.986 kb on + strand, within yahK at 342.997 kb on + strand, within yahK at 343.006 kb on + strand, within yahK at 343.032 kb on + strand, within yahK at 343.033 kb on + strand, within yahK at 343.033 kb on + strand, within yahK at 343.034 kb on + strand, within yahK at 343.040 kb on - strand, within yahK at 343.048 kb on - strand, within yahK at 343.056 kb on + strand at 343.056 kb on + strand at 343.056 kb on + strand at 343.093 kb on + strand at 343.093 kb on + strand at 343.096 kb on - strand at 343.116 kb on - strand at 343.146 kb on + strand at 343.146 kb on + strand at 343.180 kb on + strand at 343.191 kb on + strand at 343.223 kb on + strand at 343.235 kb on + strand at 343.235 kb on + strand at 343.248 kb on + strand at 343.282 kb on + strand at 343.293 kb on + strand at 343.293 kb on + strand at 343.293 kb on + strand at 343.295 kb on + strand at 343.303 kb on + strand at 343.318 kb on - strand at 343.328 kb on + strand at 343.328 kb on + strand at 343.340 kb on + strand at 343.614 kb on - strand, within yahL at 343.614 kb on - strand, within yahL at 343.617 kb on - strand, within yahL at 343.625 kb on - strand, within yahL at 343.625 kb on - strand, within yahL at 343.625 kb on - strand, within yahL at 343.625 kb on - strand, within yahL at 343.627 kb on - strand, within yahL at 343.633 kb on - strand, within yahL at 343.763 kb on + strand, within yahL at 343.767 kb on + strand, within yahL at 343.771 kb on - strand, within yahL at 343.775 kb on + strand, within yahL at 343.775 kb on + strand, within yahL at 343.775 kb on + strand, within yahL at 343.775 kb on + strand, within yahL at 343.819 kb on - strand, within yahL at 343.819 kb on - strand, within yahL at 343.852 kb on + strand, within yahL at 343.981 kb on + strand, within yahL at 343.999 kb on + strand, within yahL at 344.077 kb on - strand, within yahL at 344.080 kb on - strand, within yahL at 344.085 kb on + strand, within yahL at 344.107 kb on + strand, within yahL at 344.211 kb on - strand at 344.216 kb on - strand at 344.218 kb on - strand at 344.218 kb on - strand at 344.218 kb on - strand at 344.218 kb on - strand at 344.224 kb on + strand at 344.232 kb on - strand at 344.232 kb on - strand at 344.233 kb on - strand at 344.239 kb on - strand at 344.241 kb on - strand at 344.258 kb on - strand at 344.270 kb on + strand at 344.274 kb on - strand at 344.276 kb on - strand at 344.294 kb on + strand at 344.295 kb on - strand at 344.295 kb on - strand at 344.297 kb on - strand at 344.297 kb on - strand at 344.297 kb on - strand at 344.300 kb on - strand at 344.302 kb on - strand at 344.434 kb on + strand at 344.501 kb on - strand at 344.570 kb on + strand at 344.661 kb on + strand, within yahM at 344.661 kb on + strand, within yahM at 344.713 kb on + strand, within yahM at 344.713 kb on + strand, within yahM at 344.718 kb on + strand, within yahM at 344.718 kb on + strand, within yahM at 344.748 kb on + strand, within yahM at 344.748 kb on + strand, within yahM at 344.749 kb on + strand, within yahM at 344.751 kb on + strand, within yahM at 344.756 kb on + strand, within yahM at 344.756 kb on - strand, within yahM at 344.759 kb on - strand, within yahM at 344.828 kb on + strand, within yahM at 344.849 kb on - strand at 344.860 kb on + strand at 344.927 kb on - strand at 344.927 kb on - strand at 344.982 kb on + strand, within yahN at 344.982 kb on + strand, within yahN at 344.987 kb on + strand, within yahN at 345.056 kb on + strand, within yahN at 345.059 kb on - strand, within yahN at 345.064 kb on - strand, within yahN at 345.064 kb on - strand, within yahN at 345.065 kb on - strand, within yahN at 345.065 kb on - strand, within yahN at 345.067 kb on + strand, within yahN at 345.075 kb on - strand, within yahN at 345.078 kb on - strand, within yahN at 345.078 kb on - strand, within yahN at 345.103 kb on + strand, within yahN at 345.140 kb on + strand, within yahN at 345.146 kb on + strand, within yahN at 345.195 kb on - strand, within yahN at 345.195 kb on - strand, within yahN at 345.206 kb on + strand, within yahN at 345.210 kb on + strand, within yahN
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 342,422 + yahK b0325 0.30 +0.5 342,445 + yahK b0325 0.32 +1.2 342,488 - yahK b0325 0.36 +0.7 342,493 + yahK b0325 0.37 +0.4 342,510 + yahK b0325 0.38 -1.2 342,525 + yahK b0325 0.40 +1.4 342,525 + yahK b0325 0.40 +0.1 342,714 + yahK b0325 0.58 +1.9 342,714 + yahK b0325 0.58 -2.0 342,906 - yahK b0325 0.76 -1.1 342,979 + yahK b0325 0.83 +3.4 342,979 + yahK b0325 0.83 +2.1 342,986 + yahK b0325 0.84 -1.0 342,997 + yahK b0325 0.85 -0.4 343,006 + yahK b0325 0.86 +1.4 343,032 + yahK b0325 0.88 -0.2 343,033 + yahK b0325 0.88 -0.4 343,033 + yahK b0325 0.88 +2.2 343,034 + yahK b0325 0.88 -0.2 343,040 - yahK b0325 0.89 +0.3 343,048 - yahK b0325 0.90 -0.1 343,056 + +0.1 343,056 + -0.2 343,056 + +0.4 343,093 + +1.1 343,093 + -0.8 343,096 - +0.0 343,116 - -1.9 343,146 + +0.3 343,146 + +0.6 343,180 + +0.9 343,191 + -0.1 343,223 + -1.2 343,235 + -1.6 343,235 + +0.1 343,248 + +1.1 343,282 + -2.1 343,293 + -2.3 343,293 + +0.4 343,293 + -2.3 343,295 + -2.0 343,303 + -1.5 343,318 - -3.0 343,328 + -1.2 343,328 + -2.1 343,340 + -0.9 343,614 - yahL b0326 0.26 -0.7 343,614 - yahL b0326 0.26 -2.3 343,617 - yahL b0326 0.27 -3.3 343,625 - yahL b0326 0.28 +0.9 343,625 - yahL b0326 0.28 -1.6 343,625 - yahL b0326 0.28 -0.2 343,625 - yahL b0326 0.28 -1.4 343,627 - yahL b0326 0.28 -1.9 343,633 - yahL b0326 0.29 -4.0 343,763 + yahL b0326 0.44 -0.2 343,767 + yahL b0326 0.45 -3.4 343,771 - yahL b0326 0.45 +0.4 343,775 + yahL b0326 0.46 -1.2 343,775 + yahL b0326 0.46 -1.3 343,775 + yahL b0326 0.46 +0.1 343,775 + yahL b0326 0.46 -1.4 343,819 - yahL b0326 0.51 -1.8 343,819 - yahL b0326 0.51 -1.5 343,852 + yahL b0326 0.55 -0.7 343,981 + yahL b0326 0.71 -2.3 343,999 + yahL b0326 0.73 -2.5 344,077 - yahL b0326 0.83 -1.9 344,080 - yahL b0326 0.83 +0.8 344,085 + yahL b0326 0.84 -3.8 344,107 + yahL b0326 0.87 -0.2 344,211 - -1.3 344,216 - +2.6 344,218 - +1.1 344,218 - -0.0 344,218 - +0.6 344,218 - -0.3 344,224 + +0.2 344,232 - +2.1 344,232 - -0.9 344,233 - -0.7 344,239 - +1.1 344,241 - +0.7 344,258 - +0.8 344,270 + -2.7 344,274 - -2.3 344,276 - -1.1 344,294 + +0.2 344,295 - +0.2 344,295 - +0.6 344,297 - -0.6 344,297 - +0.7 344,297 - +0.4 344,300 - -0.0 344,302 - +0.2 344,434 + +0.1 344,501 - -2.8 344,570 + -2.0 344,661 + yahM b0327 0.13 -0.7 344,661 + yahM b0327 0.13 +0.5 344,713 + yahM b0327 0.35 +0.1 344,713 + yahM b0327 0.35 +0.2 344,718 + yahM b0327 0.37 +0.1 344,718 + yahM b0327 0.37 +1.2 344,748 + yahM b0327 0.49 +0.5 344,748 + yahM b0327 0.49 -2.3 344,749 + yahM b0327 0.49 -1.2 344,751 + yahM b0327 0.50 +0.5 344,756 + yahM b0327 0.52 +0.1 344,756 - yahM b0327 0.52 -0.6 344,759 - yahM b0327 0.53 +0.2 344,828 + yahM b0327 0.81 +1.3 344,849 - +0.5 344,860 + +0.6 344,927 - -1.6 344,927 - +1.7 344,982 + yahN b0328 0.14 +0.5 344,982 + yahN b0328 0.14 +0.8 344,987 + yahN b0328 0.14 -0.3 345,056 + yahN b0328 0.25 +0.8 345,059 - yahN b0328 0.25 -0.7 345,064 - yahN b0328 0.26 +0.9 345,064 - yahN b0328 0.26 -1.1 345,065 - yahN b0328 0.26 -1.0 345,065 - yahN b0328 0.26 +2.0 345,067 + yahN b0328 0.26 +0.5 345,075 - yahN b0328 0.28 -0.2 345,078 - yahN b0328 0.28 +1.3 345,078 - yahN b0328 0.28 +1.7 345,103 + yahN b0328 0.32 +0.3 345,140 + yahN b0328 0.37 +1.7 345,146 + yahN b0328 0.38 -1.5 345,195 - yahN b0328 0.45 -0.3 345,195 - yahN b0328 0.45 +0.8 345,206 + yahN b0328 0.47 +0.9 345,210 + yahN b0328 0.48 +1.4
Or see this region's nucleotide sequence