Strain Fitness in Escherichia coli BW25113 around b2541

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
2,668,253	+	hcaE	b2538	0.88	-0.1
2,668,253	+	hcaE	b2538	0.88	+1.0
2,668,363	+				-0.3
2,668,539	-	hcaF	b2539	0.24	+2.3
2,668,551	-	hcaF	b2539	0.27	-0.1
2,668,551	-	hcaF	b2539	0.27	+1.3
2,668,676	+	hcaF	b2539	0.51	+0.3
2,668,676	+	hcaF	b2539	0.51	-0.0
2,668,709	+	hcaF	b2539	0.57	+2.1
2,668,715	+	hcaF	b2539	0.58	-2.1
2,668,736	-	hcaF	b2539	0.62	+0.9
2,668,759	+	hcaF	b2539	0.67	+1.6
2,668,888	-				-0.0
2,668,888	-				-2.2
2,668,925	-				+0.7
2,668,932	-				-0.3
2,668,979	-	hcaC	b2540	0.15	-0.2
2,669,083	+	hcaC	b2540	0.48	-2.2
2,669,112	-	hcaC	b2540	0.57	-0.1
2,669,166	+	hcaC	b2540	0.74	+0.5
2,669,295	+				+1.1
2,669,442	-	hcaB	b2541	0.24	+0.7
2,669,469	-				-0.2
2,669,662	+	hcaB	b2541	0.51	+0.6
2,669,678	-	hcaB	b2541	0.53	-0.8
2,669,678	-	hcaB	b2541	0.53	+1.1
2,669,716	-	hcaB	b2541	0.58	-0.9
2,669,716	-	hcaB	b2541	0.58	-0.8
2,669,827	+	hcaB	b2541	0.71	-0.3
2,669,827	+	hcaB	b2541	0.71	+0.2
2,669,835	-	hcaB	b2541	0.72	-1.2
2,669,835	-	hcaB	b2541	0.72	+1.6
2,669,946	+	hcaB	b2541	0.86	+0.1
2,669,947	-	hcaB	b2541	0.86	-1.3
2,669,951	+	hcaB	b2541	0.87	+0.9
2,669,951	+	hcaB	b2541	0.87	+0.2
2,670,153	+				+1.2
2,670,199	+	hcaD	b2542	0.11	-2.0
2,670,427	-	hcaD	b2542	0.30	+0.4
2,670,427	-	hcaD	b2542	0.30	+1.6
2,670,447	+	hcaD	b2542	0.31	+1.0
2,670,450	-	hcaD	b2542	0.32	-2.0
2,670,450	-	hcaD	b2542	0.32	+0.8
2,670,576	-	hcaD	b2542	0.42	+0.1
2,670,576	-	hcaD	b2542	0.42	+0.0
2,670,576	-	hcaD	b2542	0.42	+0.2
2,670,603	+	hcaD	b2542	0.44	+1.8
2,670,836	-	hcaD	b2542	0.64	+1.3
2,670,927	-	hcaD	b2542	0.71	+1.7
2,670,994	-	hcaD	b2542	0.77	+0.6
2,670,994	-	hcaD	b2542	0.77	+1.6
2,671,037	+	hcaD	b2542	0.80	+0.1