Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdN and ybdO are separated by 146 nucleotides ybdO and dsbG are separated by 208 nucleotides
b0602: ybdN - hypothetical protein (NCBI), at 634,572 to 635,792
ybdN
b0603: ybdO - predicted DNA-binding transcriptional regulator (NCBI), at 635,939 to 636,841
ybdO
b0604: dsbG - thiol:disulfide interchange protein (VIMSS), at 637,050 to 637,796
dsbG
Position (kb) 635
636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 634.963 kb on + strand, within ybdN at 634.977 kb on + strand, within ybdN at 635.018 kb on + strand, within ybdN at 635.018 kb on + strand, within ybdN at 635.039 kb on - strand, within ybdN at 635.132 kb on + strand, within ybdN at 635.140 kb on - strand, within ybdN at 635.140 kb on - strand, within ybdN at 635.298 kb on - strand, within ybdN at 635.431 kb on - strand, within ybdN at 635.437 kb on - strand, within ybdN at 635.457 kb on - strand, within ybdN at 635.475 kb on + strand, within ybdN at 635.500 kb on + strand, within ybdN at 635.505 kb on + strand, within ybdN at 635.505 kb on + strand, within ybdN at 635.542 kb on + strand, within ybdN at 635.545 kb on - strand, within ybdN at 635.545 kb on - strand, within ybdN at 635.601 kb on + strand, within ybdN at 635.604 kb on - strand, within ybdN at 635.700 kb on + strand at 635.700 kb on + strand at 635.705 kb on + strand at 635.705 kb on + strand at 635.709 kb on + strand at 635.709 kb on + strand at 635.709 kb on + strand at 635.709 kb on + strand at 635.709 kb on - strand at 635.711 kb on + strand at 635.711 kb on + strand at 635.715 kb on + strand at 635.717 kb on - strand at 635.723 kb on + strand at 635.723 kb on + strand at 635.723 kb on + strand at 635.730 kb on + strand at 635.730 kb on + strand at 635.731 kb on - strand at 635.734 kb on + strand at 635.734 kb on + strand at 635.734 kb on + strand at 635.743 kb on + strand at 635.743 kb on + strand at 635.792 kb on + strand at 635.792 kb on + strand at 635.804 kb on + strand at 635.808 kb on + strand at 635.810 kb on + strand at 635.812 kb on + strand at 635.812 kb on + strand at 635.812 kb on + strand at 635.813 kb on + strand at 635.813 kb on + strand at 635.813 kb on + strand at 635.820 kb on - strand at 635.826 kb on + strand at 635.851 kb on + strand at 635.861 kb on + strand at 635.861 kb on + strand at 636.350 kb on - strand, within ybdO at 636.360 kb on - strand, within ybdO at 636.360 kb on - strand, within ybdO at 636.500 kb on + strand, within ybdO at 636.942 kb on - strand at 636.947 kb on - strand at 636.947 kb on - strand at 636.976 kb on - strand at 636.976 kb on - strand at 636.976 kb on - strand at 636.976 kb on - strand at 636.976 kb on - strand at 636.976 kb on - strand at 636.976 kb on - strand at 637.021 kb on - strand at 637.029 kb on - strand at 637.032 kb on - strand at 637.032 kb on - strand at 637.097 kb on - strand at 637.097 kb on - strand at 637.102 kb on - strand at 637.102 kb on - strand at 637.102 kb on - strand at 637.102 kb on - strand at 637.103 kb on - strand at 637.103 kb on - strand at 637.103 kb on - strand at 637.109 kb on - strand at 637.109 kb on - strand at 637.110 kb on - strand at 637.113 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.114 kb on - strand at 637.137 kb on - strand, within dsbG at 637.137 kb on - strand, within dsbG at 637.137 kb on - strand, within dsbG at 637.150 kb on - strand, within dsbG at 637.185 kb on + strand, within dsbG at 637.204 kb on - strand, within dsbG at 637.204 kb on - strand, within dsbG at 637.217 kb on + strand, within dsbG at 637.237 kb on - strand, within dsbG at 637.237 kb on - strand, within dsbG at 637.244 kb on + strand, within dsbG at 637.498 kb on - strand, within dsbG at 637.836 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 634,963 + ybdN b0602 0.32 -1.0 634,977 + ybdN b0602 0.33 -1.3 635,018 + ybdN b0602 0.37 -1.5 635,018 + ybdN b0602 0.37 +0.2 635,039 - ybdN b0602 0.38 -0.9 635,132 + ybdN b0602 0.46 +0.0 635,140 - ybdN b0602 0.47 -1.6 635,140 - ybdN b0602 0.47 -0.3 635,298 - ybdN b0602 0.59 +0.5 635,431 - ybdN b0602 0.70 -1.0 635,437 - ybdN b0602 0.71 -0.2 635,457 - ybdN b0602 0.72 -2.3 635,475 + ybdN b0602 0.74 -0.5 635,500 + ybdN b0602 0.76 -0.9 635,505 + ybdN b0602 0.76 -0.2 635,505 + ybdN b0602 0.76 -0.1 635,542 + ybdN b0602 0.79 -1.9 635,545 - ybdN b0602 0.80 -1.3 635,545 - ybdN b0602 0.80 -1.0 635,601 + ybdN b0602 0.84 +0.4 635,604 - ybdN b0602 0.85 -0.6 635,700 + -0.4 635,700 + -0.4 635,705 + +0.6 635,705 + -1.1 635,709 + -0.5 635,709 + -1.0 635,709 + +0.8 635,709 + +1.5 635,709 - +1.1 635,711 + -1.1 635,711 + -0.8 635,715 + -0.3 635,717 - -0.4 635,723 + +0.3 635,723 + +2.1 635,723 + +0.0 635,730 + +0.5 635,730 + +0.0 635,731 - -1.9 635,734 + -0.1 635,734 + +1.0 635,734 + -0.5 635,743 + +1.2 635,743 + -1.0 635,792 + -3.1 635,792 + -0.3 635,804 + -0.2 635,808 + -0.8 635,810 + +0.3 635,812 + -0.3 635,812 + -1.9 635,812 + -0.4 635,813 + -0.9 635,813 + +2.2 635,813 + -0.7 635,820 - -3.2 635,826 + +0.5 635,851 + -0.9 635,861 + +1.6 635,861 + -0.1 636,350 - ybdO b0603 0.46 -0.7 636,360 - ybdO b0603 0.47 -0.5 636,360 - ybdO b0603 0.47 -0.9 636,500 + ybdO b0603 0.62 -3.3 636,942 - -1.5 636,947 - +1.0 636,947 - -0.1 636,976 - -0.7 636,976 - +1.0 636,976 - -1.6 636,976 - +0.9 636,976 - +0.6 636,976 - -0.8 636,976 - -0.5 637,021 - +1.9 637,029 - -0.7 637,032 - -0.6 637,032 - -1.2 637,097 - +0.8 637,097 - +0.2 637,102 - +2.9 637,102 - +0.3 637,102 - +0.5 637,102 - +0.0 637,103 - -1.1 637,103 - -0.4 637,103 - +0.1 637,109 - +1.5 637,109 - -0.7 637,110 - +1.8 637,113 - +0.6 637,114 - -0.5 637,114 - +0.7 637,114 - +0.5 637,114 - -2.3 637,114 - +0.9 637,114 - +0.1 637,114 - +2.3 637,114 - -0.3 637,137 - dsbG b0604 0.12 +2.1 637,137 - dsbG b0604 0.12 +0.5 637,137 - dsbG b0604 0.12 +0.2 637,150 - dsbG b0604 0.13 +1.9 637,185 + dsbG b0604 0.18 +0.8 637,204 - dsbG b0604 0.21 +1.1 637,204 - dsbG b0604 0.21 -0.5 637,217 + dsbG b0604 0.22 -0.3 637,237 - dsbG b0604 0.25 -1.1 637,237 - dsbG b0604 0.25 -1.0 637,244 + dsbG b0604 0.26 +0.2 637,498 - dsbG b0604 0.60 +2.4 637,836 + +0.6
Or see this region's nucleotide sequence