Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entE and entB are separated by 13 nucleotides entB and entA overlap by 1 nucleotides entA and ybdB are separated by 2 nucleotides
b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903
entE
b0595: entB - isochorismatase (NCBI), at 626,917 to 627,774
entB
b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520
entA
b0597: ybdB - hypothetical protein (NCBI), at 628,523 to 628,936
ybdB
Position (kb) 626
627
628 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 625.958 kb on + strand, within entE at 625.964 kb on - strand, within entE at 625.964 kb on - strand, within entE at 625.971 kb on - strand, within entE at 626.073 kb on + strand, within entE at 626.303 kb on - strand, within entE at 626.303 kb on - strand, within entE at 626.369 kb on - strand, within entE at 626.487 kb on - strand, within entE at 626.643 kb on + strand, within entE at 626.643 kb on + strand, within entE at 626.751 kb on + strand at 626.751 kb on + strand at 626.851 kb on - strand at 626.896 kb on - strand at 626.908 kb on - strand at 626.908 kb on - strand at 626.937 kb on + strand at 626.937 kb on + strand at 627.113 kb on + strand, within entB at 627.113 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.367 kb on + strand, within entB at 627.437 kb on - strand, within entB at 627.437 kb on - strand, within entB at 627.550 kb on - strand, within entB at 627.610 kb on - strand, within entB at 627.619 kb on + strand, within entB at 627.619 kb on + strand, within entB at 627.629 kb on - strand, within entB at 627.706 kb on - strand at 627.751 kb on + strand at 627.825 kb on + strand at 627.950 kb on - strand, within entA at 628.176 kb on + strand, within entA at 628.200 kb on + strand, within entA at 628.208 kb on - strand, within entA at 628.219 kb on - strand, within entA at 628.257 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.297 kb on + strand, within entA at 628.297 kb on + strand, within entA at 628.319 kb on - strand, within entA at 628.387 kb on - strand, within entA at 628.463 kb on - strand at 628.463 kb on - strand at 628.601 kb on + strand, within ybdB at 628.609 kb on - strand, within ybdB at 628.618 kb on - strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 625,958 + entE b0594 0.41 +1.2 625,964 - entE b0594 0.42 -1.3 625,964 - entE b0594 0.42 +0.5 625,971 - entE b0594 0.42 +0.4 626,073 + entE b0594 0.48 +0.3 626,303 - entE b0594 0.63 +1.4 626,303 - entE b0594 0.63 -0.1 626,369 - entE b0594 0.67 -0.5 626,487 - entE b0594 0.74 +1.4 626,643 + entE b0594 0.84 -0.2 626,643 + entE b0594 0.84 -0.6 626,751 + +1.5 626,751 + +1.8 626,851 - +1.6 626,896 - -0.1 626,908 - +0.2 626,908 - -1.4 626,937 + +0.5 626,937 + +0.9 627,113 + entB b0595 0.23 +0.9 627,113 + entB b0595 0.23 +1.4 627,128 + entB b0595 0.25 +0.4 627,128 + entB b0595 0.25 +0.9 627,146 - entB b0595 0.27 +3.4 627,146 - entB b0595 0.27 +0.7 627,146 - entB b0595 0.27 +0.6 627,146 - entB b0595 0.27 -1.5 627,216 - entB b0595 0.35 +0.7 627,216 - entB b0595 0.35 +0.2 627,367 + entB b0595 0.52 +0.8 627,437 - entB b0595 0.61 -0.1 627,437 - entB b0595 0.61 +0.2 627,550 - entB b0595 0.74 +0.6 627,610 - entB b0595 0.81 +2.1 627,619 + entB b0595 0.82 +1.1 627,619 + entB b0595 0.82 +3.2 627,629 - entB b0595 0.83 +0.1 627,706 - +1.6 627,751 + -0.4 627,825 + +1.4 627,950 - entA b0596 0.24 -0.6 628,176 + entA b0596 0.54 +1.2 628,200 + entA b0596 0.57 +3.0 628,208 - entA b0596 0.58 +2.2 628,219 - entA b0596 0.60 +0.3 628,257 - entA b0596 0.65 -0.7 628,287 - entA b0596 0.69 +0.9 628,287 - entA b0596 0.69 -0.4 628,297 + entA b0596 0.70 -0.3 628,297 + entA b0596 0.70 +2.8 628,319 - entA b0596 0.73 +1.1 628,387 - entA b0596 0.82 +1.7 628,463 - -0.5 628,463 - -2.8 628,601 + ybdB b0597 0.19 +0.3 628,609 - ybdB b0597 0.21 +0.4 628,618 - ybdB b0597 0.23 -2.3 628,640 + ybdB b0597 0.28 -0.7 628,640 + ybdB b0597 0.28 -1.2 628,756 + ybdB b0597 0.56 -2.4 628,756 + ybdB b0597 0.56 +1.4
Or see this region's nucleotide sequence