Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiB and caiA are separated by 128 nucleotides caiA and caiT are separated by 30 nucleotides
b0038: caiB - crotonobetainyl-CoA:carnitineCoA-transferase (NCBI), at 37,898 to 39,115
caiB
b0039: caiA - crotonobetaine reductase subunit II, FAD-binding (NCBI), at 39,244 to 40,386
caiA
b0040: caiT - L-carnitine/gamma-butyrobetaine antiporter (NCBI), at 40,417 to 41,931
caiT
Position (kb) 39
40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 38.279 kb on - strand, within caiB at 38.279 kb on - strand, within caiB at 38.286 kb on - strand, within caiB at 38.314 kb on + strand, within caiB at 38.325 kb on - strand, within caiB at 38.340 kb on - strand, within caiB at 38.361 kb on - strand, within caiB at 38.361 kb on - strand, within caiB at 38.368 kb on + strand, within caiB at 38.368 kb on - strand, within caiB at 38.372 kb on + strand, within caiB at 38.394 kb on + strand, within caiB at 38.428 kb on - strand, within caiB at 38.482 kb on - strand, within caiB at 38.494 kb on - strand, within caiB at 38.516 kb on - strand, within caiB at 38.564 kb on - strand, within caiB at 38.599 kb on - strand, within caiB at 38.699 kb on - strand, within caiB at 38.724 kb on + strand, within caiB at 38.732 kb on - strand, within caiB at 38.732 kb on - strand, within caiB at 38.771 kb on + strand, within caiB at 38.771 kb on + strand, within caiB at 38.829 kb on - strand, within caiB at 38.829 kb on - strand, within caiB at 38.831 kb on + strand, within caiB at 38.881 kb on - strand, within caiB at 38.941 kb on - strand, within caiB at 39.052 kb on - strand at 39.052 kb on - strand at 39.072 kb on + strand at 39.101 kb on + strand at 39.101 kb on + strand at 39.177 kb on - strand at 39.194 kb on + strand at 39.195 kb on + strand at 39.195 kb on + strand at 39.264 kb on - strand at 39.272 kb on - strand at 39.272 kb on - strand at 39.279 kb on - strand at 39.439 kb on - strand, within caiA at 39.472 kb on - strand, within caiA at 39.496 kb on - strand, within caiA at 39.589 kb on + strand, within caiA at 39.640 kb on - strand, within caiA at 39.640 kb on - strand, within caiA at 39.746 kb on - strand, within caiA at 39.800 kb on - strand, within caiA at 39.800 kb on - strand, within caiA at 39.849 kb on + strand, within caiA at 39.910 kb on - strand, within caiA at 39.982 kb on - strand, within caiA at 39.982 kb on - strand, within caiA at 40.143 kb on + strand, within caiA at 40.143 kb on + strand, within caiA at 40.179 kb on + strand, within caiA at 40.179 kb on + strand, within caiA at 40.179 kb on + strand, within caiA at 40.195 kb on + strand, within caiA at 40.203 kb on - strand, within caiA at 40.227 kb on - strand, within caiA at 40.227 kb on - strand, within caiA at 40.529 kb on - strand at 40.540 kb on + strand at 40.545 kb on + strand at 40.591 kb on + strand, within caiT at 40.646 kb on + strand, within caiT at 40.646 kb on + strand, within caiT at 40.646 kb on + strand, within caiT at 40.654 kb on - strand, within caiT at 40.654 kb on - strand, within caiT at 40.668 kb on + strand, within caiT at 40.668 kb on + strand, within caiT at 40.701 kb on - strand, within caiT at 40.766 kb on - strand, within caiT at 40.766 kb on - strand, within caiT at 40.774 kb on - strand, within caiT at 40.839 kb on - strand, within caiT at 40.850 kb on + strand, within caiT at 40.889 kb on - strand, within caiT at 40.902 kb on - strand, within caiT at 40.902 kb on - strand, within caiT at 40.902 kb on - strand, within caiT at 40.908 kb on + strand, within caiT at 40.912 kb on - strand, within caiT at 40.918 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.947 kb on - strand, within caiT at 40.972 kb on + strand, within caiT at 41.029 kb on - strand, within caiT at 41.029 kb on - strand, within caiT at 41.146 kb on + strand, within caiT at 41.158 kb on + strand, within caiT at 41.158 kb on + strand, within caiT at 41.179 kb on - strand, within caiT at 41.256 kb on - strand, within caiT at 41.282 kb on - strand, within caiT at 41.318 kb on - strand, within caiT at 41.329 kb on + strand, within caiT
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 38,279 - caiB b0038 0.31 +0.4 38,279 - caiB b0038 0.31 +1.3 38,286 - caiB b0038 0.32 +0.6 38,314 + caiB b0038 0.34 -2.1 38,325 - caiB b0038 0.35 -1.7 38,340 - caiB b0038 0.36 +0.4 38,361 - caiB b0038 0.38 +1.2 38,361 - caiB b0038 0.38 +0.5 38,368 + caiB b0038 0.39 -0.9 38,368 - caiB b0038 0.39 -0.1 38,372 + caiB b0038 0.39 +0.6 38,394 + caiB b0038 0.41 +2.4 38,428 - caiB b0038 0.44 +0.1 38,482 - caiB b0038 0.48 +0.7 38,494 - caiB b0038 0.49 +0.2 38,516 - caiB b0038 0.51 -0.2 38,564 - caiB b0038 0.55 -0.0 38,599 - caiB b0038 0.58 -0.3 38,699 - caiB b0038 0.66 -0.2 38,724 + caiB b0038 0.68 +0.3 38,732 - caiB b0038 0.68 -1.3 38,732 - caiB b0038 0.68 -0.5 38,771 + caiB b0038 0.72 +0.7 38,771 + caiB b0038 0.72 +3.0 38,829 - caiB b0038 0.76 +1.2 38,829 - caiB b0038 0.76 -0.1 38,831 + caiB b0038 0.77 +0.6 38,881 - caiB b0038 0.81 +2.5 38,941 - caiB b0038 0.86 +1.0 39,052 - -1.1 39,052 - -0.8 39,072 + -0.2 39,101 + +1.8 39,101 + +0.9 39,177 - -1.2 39,194 + -3.3 39,195 + +0.2 39,195 + -1.3 39,264 - -0.4 39,272 - -2.4 39,272 - -1.1 39,279 - +2.6 39,439 - caiA b0039 0.17 -0.1 39,472 - caiA b0039 0.20 +2.0 39,496 - caiA b0039 0.22 -1.1 39,589 + caiA b0039 0.30 +0.9 39,640 - caiA b0039 0.35 +0.1 39,640 - caiA b0039 0.35 -1.8 39,746 - caiA b0039 0.44 -0.8 39,800 - caiA b0039 0.49 +2.9 39,800 - caiA b0039 0.49 -3.1 39,849 + caiA b0039 0.53 +1.1 39,910 - caiA b0039 0.58 -0.6 39,982 - caiA b0039 0.65 +1.8 39,982 - caiA b0039 0.65 +2.2 40,143 + caiA b0039 0.79 +1.4 40,143 + caiA b0039 0.79 +0.5 40,179 + caiA b0039 0.82 +0.0 40,179 + caiA b0039 0.82 +0.8 40,179 + caiA b0039 0.82 +1.0 40,195 + caiA b0039 0.83 +0.3 40,203 - caiA b0039 0.84 +1.4 40,227 - caiA b0039 0.86 +1.4 40,227 - caiA b0039 0.86 -1.1 40,529 - -1.0 40,540 + +0.6 40,545 + +1.6 40,591 + caiT b0040 0.11 +0.5 40,646 + caiT b0040 0.15 -0.9 40,646 + caiT b0040 0.15 +0.2 40,646 + caiT b0040 0.15 +0.1 40,654 - caiT b0040 0.16 -2.6 40,654 - caiT b0040 0.16 -1.3 40,668 + caiT b0040 0.17 +0.1 40,668 + caiT b0040 0.17 -0.5 40,701 - caiT b0040 0.19 -1.3 40,766 - caiT b0040 0.23 +0.8 40,766 - caiT b0040 0.23 -1.7 40,774 - caiT b0040 0.24 -0.4 40,839 - caiT b0040 0.28 -0.3 40,850 + caiT b0040 0.29 +1.0 40,889 - caiT b0040 0.31 -0.4 40,902 - caiT b0040 0.32 +2.3 40,902 - caiT b0040 0.32 -1.1 40,902 - caiT b0040 0.32 +0.3 40,908 + caiT b0040 0.32 -0.5 40,912 - caiT b0040 0.33 -0.1 40,918 - caiT b0040 0.33 +1.6 40,947 - caiT b0040 0.35 +0.3 40,947 - caiT b0040 0.35 +1.1 40,947 - caiT b0040 0.35 +3.4 40,947 - caiT b0040 0.35 -0.5 40,972 + caiT b0040 0.37 +0.6 41,029 - caiT b0040 0.40 +0.0 41,029 - caiT b0040 0.40 +0.8 41,146 + caiT b0040 0.48 +0.9 41,158 + caiT b0040 0.49 -1.6 41,158 + caiT b0040 0.49 +0.8 41,179 - caiT b0040 0.50 +2.2 41,256 - caiT b0040 0.55 -0.2 41,282 - caiT b0040 0.57 +1.6 41,318 - caiT b0040 0.59 -1.1 41,329 + caiT b0040 0.60 -0.3
Or see this region's nucleotide sequence