Strain Fitness in Escherichia coli BW25113 around b4319

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
4,545,345	+	fimD	b4317	0.84	+1.0
4,545,354	-	fimD	b4317	0.85	-1.3
4,545,354	-	fimD	b4317	0.85	-0.1
4,545,367	-	fimD	b4317	0.85	-1.1
4,545,391	-	fimD	b4317	0.86	+0.2
4,545,393	+	fimD	b4317	0.86	-1.9
4,545,401	-	fimD	b4317	0.87	+1.2
4,545,403	-	fimD	b4317	0.87	-0.2
4,545,468	-	fimD	b4317	0.89	+0.7
4,545,468	-	fimD	b4317	0.89	+0.5
4,545,485	+	fimD	b4317	0.90	+0.4
4,545,519	+				-0.4
4,545,555	-				-2.0
4,545,555	-				-0.2
4,545,565	+				-0.7
4,545,612	-				-0.4
4,545,724	+				+2.2
4,545,734	+				-0.7
4,545,759	+				+1.6
4,545,759	+				+0.0
4,545,874	+				+3.5
4,546,062	-	fimF	b4318	0.56	-0.1
4,546,062	-				+2.6
4,546,251	-				-0.0
4,546,251	-				-1.0
4,546,259	-				+0.6
4,546,289	-				+0.9
4,546,292	+				+0.6
4,546,365	-	fimG	b4319	0.11	-1.4
4,546,373	-	fimG	b4319	0.13	+0.3
4,546,373	-	fimG	b4319	0.13	-1.2
4,546,373	-	fimG	b4319	0.13	-0.2
4,546,411	+	fimG	b4319	0.20	+0.1
4,546,419	-	fimG	b4319	0.22	+0.4
4,546,429	-				+0.2
4,546,511	-	fimG	b4319	0.40	-0.6
4,546,511	-	fimG	b4319	0.40	-0.3
4,546,643	-	fimG	b4319	0.66	-0.1
4,546,647	+	fimG	b4319	0.67	-0.1
4,546,650	+	fimG	b4319	0.68	+0.1
4,546,657	-	fimG	b4319	0.69	+0.0
4,546,669	-	fimG	b4319	0.72	-0.9
4,546,669	-	fimG	b4319	0.72	-0.7
4,546,684	-	fimG	b4319	0.75	+1.3
4,546,794	+				+0.3
4,546,828	+				+0.0
4,546,859	-				-0.9
4,546,871	-				+0.6
4,546,874	-				-1.0
4,546,882	-				-0.3
4,546,882	-				-0.2
4,547,047	+	fimH	b4320	0.24	+1.1
4,547,047	+	fimH	b4320	0.24	+1.9
4,547,055	+	fimH	b4320	0.25	-1.2
4,547,055	+	fimH	b4320	0.25	+0.6
4,547,057	+	fimH	b4320	0.25	-1.1
4,547,057	+	fimH	b4320	0.25	-0.5
4,547,057	+	fimH	b4320	0.25	-1.2
4,547,059	+	fimH	b4320	0.25	-0.6
4,547,065	-	fimH	b4320	0.26	+0.7
4,547,066	-	fimH	b4320	0.26	-0.3
4,547,067	+	fimH	b4320	0.26	+0.6
4,547,080	+	fimH	b4320	0.28	+0.3
4,547,080	+	fimH	b4320	0.28	-1.1
4,547,080	+	fimH	b4320	0.28	+0.9
4,547,080	+	fimH	b4320	0.28	-0.0
4,547,145	+	fimH	b4320	0.35	-2.2
4,547,224	+	fimH	b4320	0.44	-0.4
4,547,232	-	fimH	b4320	0.44	+0.8
4,547,232	-	fimH	b4320	0.44	-0.8
4,547,326	+	fimH	b4320	0.55	+0.6
4,547,361	+	fimH	b4320	0.59	+0.5
4,547,361	+	fimH	b4320	0.59	-0.2
4,547,361	+	fimH	b4320	0.59	-0.5
4,547,361	+	fimH	b4320	0.59	+0.8
4,547,363	+	fimH	b4320	0.59	-0.1
4,547,367	-	fimH	b4320	0.59	+2.2
4,547,367	-	fimH	b4320	0.59	-1.3
4,547,369	-	fimH	b4320	0.60	+0.5
4,547,369	-	fimH	b4320	0.60	-2.0
4,547,369	-	fimH	b4320	0.60	+1.8
4,547,387	+	fimH	b4320	0.62	+0.1
4,547,387	+	fimH	b4320	0.62	+1.2
4,547,407	+	fimH	b4320	0.64	-1.3
4,547,407	+	fimH	b4320	0.64	+0.6
4,547,417	+	fimH	b4320	0.65	+0.9
4,547,417	+	fimH	b4320	0.65	+0.2
4,547,417	+	fimH	b4320	0.65	+2.2
4,547,425	-	fimH	b4320	0.66	+3.6
4,547,481	-	fimH	b4320	0.72	+1.1
4,547,483	-	fimH	b4320	0.72	+0.1
4,547,490	-	fimH	b4320	0.73	-0.7
4,547,490	-	fimH	b4320	0.73	+5.0
4,547,542	+	fimH	b4320	0.79	-0.8
4,547,542	+	fimH	b4320	0.79	-0.5
4,547,558	-	fimH	b4320	0.81	-1.3
4,547,565	+	fimH	b4320	0.81	+2.2
4,547,647	-				+0.6
4,547,647	-				+1.2
4,547,666	+				-0.0
4,547,747	-				+0.1
4,547,763	+				-1.5
4,547,766	+				+0.7
4,547,766	-				+1.1
4,547,768	+				+0.0