Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdcR and yhaB are separated by 255 nucleotides yhaB and yhaC are separated by 21 nucleotides yhaC and rnpB are separated by 613 nucleotides
b3119: tdcR - threonine dehydratase operon activator protein (VIMSS), at 3,265,402 to 3,265,620
tdcR
b3120: yhaB - orf, hypothetical protein (VIMSS), at 3,265,876 to 3,266,415
yhaB
b3121: yhaC - hypothetical protein (NCBI), at 3,266,437 to 3,267,624
yhaC
b3123: rnpB - RNase P RNA component precursor RnpB (NCBI), at 3,268,238 to 3,268,614
rnpB
Position (kb)
3266
3267
3268 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3265.544 kb on - strand, within tdcR at 3265.544 kb on - strand, within tdcR at 3265.594 kb on - strand, within tdcR at 3265.658 kb on - strand at 3265.658 kb on - strand at 3265.671 kb on - strand at 3265.673 kb on + strand at 3265.698 kb on + strand at 3265.702 kb on - strand at 3265.708 kb on - strand at 3265.708 kb on - strand at 3265.743 kb on - strand at 3265.743 kb on - strand at 3265.745 kb on + strand at 3265.746 kb on + strand at 3265.746 kb on + strand at 3265.746 kb on + strand at 3265.746 kb on + strand at 3265.746 kb on + strand at 3265.751 kb on + strand at 3265.751 kb on + strand at 3265.751 kb on + strand at 3265.754 kb on - strand at 3265.758 kb on + strand at 3265.759 kb on - strand at 3265.759 kb on - strand at 3265.759 kb on - strand at 3265.763 kb on - strand at 3265.763 kb on - strand at 3265.768 kb on - strand at 3265.778 kb on - strand at 3265.778 kb on - strand at 3265.798 kb on + strand at 3265.798 kb on + strand at 3265.798 kb on + strand at 3265.806 kb on - strand at 3265.842 kb on + strand at 3265.881 kb on - strand at 3265.914 kb on + strand at 3265.914 kb on + strand at 3266.009 kb on - strand, within yhaB at 3266.085 kb on - strand, within yhaB at 3266.231 kb on - strand, within yhaB at 3266.231 kb on - strand at 3266.324 kb on + strand, within yhaB at 3266.326 kb on + strand, within yhaB at 3266.334 kb on + strand, within yhaB at 3266.334 kb on + strand, within yhaB at 3266.895 kb on + strand, within yhaC at 3266.905 kb on + strand, within yhaC at 3267.596 kb on - strand at 3267.683 kb on - strand at 3267.683 kb on - strand at 3267.683 kb on - strand at 3267.683 kb on - strand at 3267.683 kb on - strand at 3267.683 kb on - strand at 3267.686 kb on - strand at 3267.724 kb on - strand at 3267.750 kb on - strand at 3267.764 kb on - strand at 3267.774 kb on - strand at 3267.795 kb on - strand at 3267.795 kb on - strand at 3267.815 kb on + strand at 3267.815 kb on + strand at 3267.823 kb on - strand at 3267.844 kb on + strand at 3267.943 kb on - strand at 3267.960 kb on + strand at 3268.053 kb on + strand at 3268.054 kb on - strand at 3268.133 kb on - strand at 3268.133 kb on - strand at 3268.160 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,265,544 - tdcR b3119 0.65 +0.6 3,265,544 - tdcR b3119 0.65 -0.9 3,265,594 - tdcR b3119 0.88 -1.8 3,265,658 - +0.8 3,265,658 - -0.6 3,265,671 - +1.0 3,265,673 + -1.5 3,265,698 + +0.5 3,265,702 - -0.9 3,265,708 - -0.0 3,265,708 - -1.6 3,265,743 - -2.8 3,265,743 - +0.7 3,265,745 + -0.6 3,265,746 + -0.5 3,265,746 + -0.1 3,265,746 + -1.1 3,265,746 + +0.2 3,265,746 + -0.8 3,265,751 + -0.3 3,265,751 + -0.3 3,265,751 + -1.3 3,265,754 - -1.6 3,265,758 + -0.2 3,265,759 - +0.9 3,265,759 - +0.8 3,265,759 - -1.8 3,265,763 - -1.4 3,265,763 - -0.3 3,265,768 - -1.4 3,265,778 - +0.7 3,265,778 - -0.4 3,265,798 + +1.1 3,265,798 + -0.2 3,265,798 + -1.9 3,265,806 - -0.8 3,265,842 + +0.0 3,265,881 - +0.5 3,265,914 + -1.1 3,265,914 + -0.5 3,266,009 - yhaB b3120 0.25 -0.6 3,266,085 - yhaB b3120 0.39 -1.2 3,266,231 - yhaB b3120 0.66 +0.4 3,266,231 - +0.3 3,266,324 + yhaB b3120 0.83 -0.8 3,266,326 + yhaB b3120 0.83 +0.1 3,266,334 + yhaB b3120 0.85 -2.7 3,266,334 + yhaB b3120 0.85 -1.2 3,266,895 + yhaC b3121 0.39 -1.5 3,266,905 + yhaC b3121 0.39 -0.6 3,267,596 - -2.3 3,267,683 - -0.7 3,267,683 - +0.9 3,267,683 - +0.2 3,267,683 - -0.5 3,267,683 - -0.7 3,267,683 - -0.7 3,267,686 - +1.6 3,267,724 - +0.3 3,267,750 - +0.1 3,267,764 - +1.0 3,267,774 - +1.3 3,267,795 - -1.1 3,267,795 - +1.8 3,267,815 + -3.1 3,267,815 + +0.6 3,267,823 - +1.1 3,267,844 + +0.8 3,267,943 - +1.4 3,267,960 + +0.3 3,268,053 + -1.3 3,268,054 - -0.1 3,268,133 - +1.3 3,268,133 - -1.6 3,268,160 - -1.5
Or see this region's nucleotide sequence