Strain Fitness in Escherichia coli BW25113 around b2771

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
2,893,566	-	ygcQ	b2769	0.73	+0.5
2,893,636	-	ygcQ	b2769	0.81	+1.4
2,893,657	-	ygcQ	b2769	0.83	-0.4
2,893,717	+				+0.5
2,893,825	+				+2.4
2,893,825	+				+0.3
2,893,883	-	ygcR	b2770	0.11	+0.7
2,893,891	-	ygcR	b2770	0.12	-0.4
2,893,896	-	ygcR	b2770	0.13	-0.2
2,893,959	+	ygcR	b2770	0.21	-0.5
2,894,040	+	ygcR	b2770	0.31	-1.1
2,894,040	+	ygcR	b2770	0.31	-0.2
2,894,141	-	ygcR	b2770	0.44	-1.4
2,894,260	-	ygcR	b2770	0.59	-0.0
2,894,334	-	ygcR	b2770	0.69	-0.4
2,894,334	-	ygcR	b2770	0.69	-1.5
2,894,362	+	ygcR	b2770	0.72	-0.9
2,894,372	+	ygcR	b2770	0.74	-2.4
2,894,372	+	ygcR	b2770	0.74	+0.3
2,894,373	-	ygcR	b2770	0.74	+0.5
2,894,373	-	ygcR	b2770	0.74	-1.0
2,894,500	-				+2.9
2,894,638	+				+2.4
2,894,756	+	ygcS	b2771	0.15	-0.7
2,894,873	+	ygcS	b2771	0.24	+1.5
2,894,873	+	ygcS	b2771	0.24	+1.9
2,894,892	+	ygcS	b2771	0.25	+0.5
2,894,999	-	ygcS	b2771	0.33	+1.0
2,895,130	-	ygcS	b2771	0.43	-0.6
2,895,187	+	ygcS	b2771	0.47	+1.3
2,895,187	+	ygcS	b2771	0.47	-0.3
2,895,442	-	ygcS	b2771	0.66	-0.3
2,895,535	-	ygcS	b2771	0.73	-1.5
2,895,559	+	ygcS	b2771	0.75	+0.7
2,895,692	-	ygcS	b2771	0.85	-0.6
2,895,721	+	ygcS	b2771	0.87	-2.3
2,895,770	+				-0.1
2,895,803	+				-0.5
2,895,906	-				+1.3
2,896,019	-				-0.6
2,896,053	+				+1.1
2,896,053	+				-1.8
2,896,084	-				-0.4
2,896,170	+	ygcU	b4463	0.13	-0.8
2,896,305	-	ygcU	b4463	0.22	+0.9
2,896,357	-	ygcU	b4463	0.25	-0.5
2,896,357	-	ygcU	b4463	0.25	+1.5
2,896,423	+	ygcU	b4463	0.30	+0.5
2,896,423	+	ygcU	b4463	0.30	+0.7
2,896,531	-	ygcU	b4463	0.37	+1.6
2,896,652	-	ygcU	b4463	0.46	-0.4
2,896,732	-	ygcU	b4463	0.51	-0.1
2,896,825	-	ygcU	b4463	0.58	-1.3