Strain Fitness in Escherichia coli BW25113 around b1689

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiK and rprA are separated by 185 nucleotidesrprA and b1689 are separated by 137 nucleotidesb1689 and ydiM are separated by 99 nucleotides b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210 ydiK b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501 rprA b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995 b1689 b1690: ydiM - predicted transporter (NCBI), at 1,769,095 to 1,770,309 ydiM Position (kb) 1768 1769Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1767.747 kb on + strand, within ydiKat 1767.784 kb on - strand, within ydiKat 1767.814 kb on - strand, within ydiKat 1767.850 kb on - strand, within ydiKat 1767.962 kb on - strand, within ydiKat 1768.086 kb on - strand, within ydiKat 1768.225 kb on + strandat 1768.225 kb on + strandat 1768.227 kb on + strandat 1768.290 kb on - strandat 1768.307 kb on + strandat 1768.307 kb on + strandat 1768.312 kb on - strandat 1768.382 kb on + strandat 1768.390 kb on - strandat 1768.466 kb on - strand, within rprAat 1768.471 kb on - strand, within rprAat 1768.479 kb on + strand, within rprAat 1768.516 kb on + strandat 1768.551 kb on + strandat 1768.559 kb on - strandat 1768.561 kb on + strandat 1768.561 kb on + strandat 1768.569 kb on + strandat 1768.569 kb on + strandat 1768.569 kb on - strandat 1768.571 kb on + strandat 1768.654 kb on + strandat 1768.661 kb on - strandat 1768.685 kb on + strand, within b1689at 1768.714 kb on - strand, within b1689at 1768.720 kb on + strand, within b1689at 1768.765 kb on + strand, within b1689at 1768.765 kb on + strand, within b1689at 1768.781 kb on - strand, within b1689at 1768.788 kb on + strand, within b1689at 1768.788 kb on + strand, within b1689at 1768.905 kb on + strand, within b1689at 1768.905 kb on + strand, within b1689at 1768.905 kb on + strand, within b1689at 1768.913 kb on - strand, within b1689at 1768.976 kb on + strandat 1769.159 kb on - strandat 1769.186 kb on - strandat 1769.198 kb on + strandat 1769.223 kb on + strand, within ydiMat 1769.230 kb on + strand, within ydiMat 1769.238 kb on - strand, within ydiMat 1769.244 kb on + strand, within ydiMat 1769.244 kb on + strand, within ydiMat 1769.323 kb on + strand, within ydiMat 1769.331 kb on - strand, within ydiMat 1769.341 kb on - strand, within ydiMat 1769.401 kb on + strand, within ydiMat 1769.461 kb on + strand, within ydiMat 1769.464 kb on + strand, within ydiMat 1769.548 kb on - strand, within ydiMat 1769.556 kb on - strand, within ydiMat 1769.561 kb on - strand, within ydiMat 1769.566 kb on - strand, within ydiMat 1769.641 kb on - strand, within ydiMat 1769.646 kb on + strand, within ydiMat 1769.653 kb on - strand, within ydiMat 1769.751 kb on - strand, within ydiMat 1769.770 kb on - strand, within ydiMat 1769.791 kb on - strand, within ydiMat 1769.817 kb on + strand, within ydiMat 1769.817 kb on + strand, within ydiMat 1769.817 kb on - strand, within ydiMat 1769.847 kb on - strand, within ydiMat 1769.847 kb on - strand, within ydiMat 1769.942 kb on - strand, within ydiMat 1769.942 kb on - strand, within ydiMat 1769.951 kb on + strand, within ydiM

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,767,747 + ydiK b1688 0.58 +0.1
1,767,784 - ydiK b1688 0.62 +0.0
1,767,814 - ydiK b1688 0.64 -0.3
1,767,850 - ydiK b1688 0.68 +0.3
1,767,962 - ydiK b1688 0.78 -2.3
1,768,086 - ydiK b1688 0.89 -1.9
1,768,225 + -1.3
1,768,225 + +1.4
1,768,227 + +0.1
1,768,290 - +0.3
1,768,307 + -0.3
1,768,307 + +0.9
1,768,312 - +1.1
1,768,382 + +0.2
1,768,390 - +0.2
1,768,466 - rprA b4431 0.66 -0.5
1,768,471 - rprA b4431 0.71 -0.3
1,768,479 + rprA b4431 0.78 -1.1
1,768,516 + +1.2
1,768,551 + +1.1
1,768,559 - -0.3
1,768,561 + +1.2
1,768,561 + +0.2
1,768,569 + +1.3
1,768,569 + -2.3
1,768,569 - -0.6
1,768,571 + +0.4
1,768,654 + +1.5
1,768,661 - -0.1
1,768,685 + b1689 b1689 0.13 +0.1
1,768,714 - b1689 b1689 0.21 +0.0
1,768,720 + b1689 b1689 0.23 -0.0
1,768,765 + b1689 b1689 0.35 -1.4
1,768,765 + b1689 b1689 0.35 -0.1
1,768,781 - b1689 b1689 0.40 -0.5
1,768,788 + b1689 b1689 0.42 +0.1
1,768,788 + b1689 b1689 0.42 +0.8
1,768,905 + b1689 b1689 0.75 -0.1
1,768,905 + b1689 b1689 0.75 +1.7
1,768,905 + b1689 b1689 0.75 +1.3
1,768,913 - b1689 b1689 0.77 -2.0
1,768,976 + -0.8
1,769,159 - -0.7
1,769,186 - -2.3
1,769,198 + -2.7
1,769,223 + ydiM b1690 0.11 -2.9
1,769,230 + ydiM b1690 0.11 -1.0
1,769,238 - ydiM b1690 0.12 -3.0
1,769,244 + ydiM b1690 0.12 +2.3
1,769,244 + ydiM b1690 0.12 -0.4
1,769,323 + ydiM b1690 0.19 +0.0
1,769,331 - ydiM b1690 0.19 -0.3
1,769,341 - ydiM b1690 0.20 +0.3
1,769,401 + ydiM b1690 0.25 +0.5
1,769,461 + ydiM b1690 0.30 +0.2
1,769,464 + ydiM b1690 0.30 -0.1
1,769,548 - ydiM b1690 0.37 -0.5
1,769,556 - ydiM b1690 0.38 +1.5
1,769,561 - ydiM b1690 0.38 +2.0
1,769,566 - ydiM b1690 0.39 -3.0
1,769,641 - ydiM b1690 0.45 +0.6
1,769,646 + ydiM b1690 0.45 -1.5
1,769,653 - ydiM b1690 0.46 -0.9
1,769,751 - ydiM b1690 0.54 -1.3
1,769,770 - ydiM b1690 0.56 +0.3
1,769,791 - ydiM b1690 0.57 -0.6
1,769,817 + ydiM b1690 0.59 -0.7
1,769,817 + ydiM b1690 0.59 -0.5
1,769,817 - ydiM b1690 0.59 -0.7
1,769,847 - ydiM b1690 0.62 +0.0
1,769,847 - ydiM b1690 0.62 -1.6
1,769,942 - ydiM b1690 0.70 -3.4
1,769,942 - ydiM b1690 0.70 -2.0
1,769,951 + ydiM b1690 0.70 +0.8

Or see this region's nucleotide sequence