Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydiJ and ydiK are separated by 388 nucleotides ydiK and rprA are separated by 185 nucleotides rprA and b1689 are separated by 137 nucleotides b1689 and ydiM are separated by 99 nucleotides
b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709
ydiJ
b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210
ydiK
b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501
rprA
b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995
b1689
b1690: ydiM - predicted transporter (NCBI), at 1,769,095 to 1,770,309
ydiM
Position (kb)
1767
1768
1769 Strain fitness (log2 ratio)
-2
-1
0
1 at 1766.258 kb on - strand, within ydiJ at 1766.258 kb on - strand, within ydiJ at 1766.301 kb on + strand, within ydiJ at 1766.335 kb on - strand, within ydiJ at 1766.385 kb on + strand, within ydiJ at 1766.385 kb on + strand, within ydiJ at 1766.465 kb on + strand at 1766.562 kb on - strand at 1766.562 kb on - strand at 1766.608 kb on + strand at 1766.730 kb on + strand at 1766.744 kb on - strand at 1766.744 kb on - strand at 1766.831 kb on - strand at 1766.897 kb on + strand at 1767.033 kb on - strand at 1767.033 kb on - strand at 1767.043 kb on - strand at 1767.115 kb on + strand at 1767.250 kb on + strand, within ydiK at 1767.471 kb on - strand, within ydiK at 1767.542 kb on + strand, within ydiK at 1767.589 kb on + strand, within ydiK at 1767.631 kb on - strand, within ydiK at 1767.747 kb on + strand, within ydiK at 1767.784 kb on - strand, within ydiK at 1767.814 kb on - strand, within ydiK at 1767.850 kb on - strand, within ydiK at 1767.962 kb on - strand, within ydiK at 1768.086 kb on - strand, within ydiK at 1768.225 kb on + strand at 1768.225 kb on + strand at 1768.227 kb on + strand at 1768.290 kb on - strand at 1768.307 kb on + strand at 1768.307 kb on + strand at 1768.312 kb on - strand at 1768.382 kb on + strand at 1768.390 kb on - strand at 1768.466 kb on - strand, within rprA at 1768.471 kb on - strand, within rprA at 1768.479 kb on + strand, within rprA at 1768.516 kb on + strand at 1768.551 kb on + strand at 1768.559 kb on - strand at 1768.561 kb on + strand at 1768.561 kb on + strand at 1768.569 kb on + strand at 1768.569 kb on + strand at 1768.569 kb on - strand at 1768.571 kb on + strand at 1768.654 kb on + strand at 1768.661 kb on - strand at 1768.685 kb on + strand, within b1689 at 1768.714 kb on - strand, within b1689 at 1768.720 kb on + strand, within b1689 at 1768.765 kb on + strand, within b1689 at 1768.765 kb on + strand, within b1689 at 1768.781 kb on - strand, within b1689 at 1768.788 kb on + strand, within b1689 at 1768.788 kb on + strand, within b1689 at 1768.905 kb on + strand, within b1689 at 1768.905 kb on + strand, within b1689 at 1768.905 kb on + strand, within b1689 at 1768.913 kb on - strand, within b1689 at 1768.976 kb on + strand at 1769.159 kb on - strand at 1769.186 kb on - strand at 1769.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,766,258 - ydiJ b1687 0.85 -0.9 1,766,258 - ydiJ b1687 0.85 -0.1 1,766,301 + ydiJ b1687 0.87 -0.0 1,766,335 - ydiJ b1687 0.88 -0.1 1,766,385 + ydiJ b1687 0.89 -0.1 1,766,385 + ydiJ b1687 0.89 -0.2 1,766,465 + +0.5 1,766,562 - -0.2 1,766,562 - -0.5 1,766,608 + -1.2 1,766,730 + +0.1 1,766,744 - -0.0 1,766,744 - +0.6 1,766,831 - -0.3 1,766,897 + +0.3 1,767,033 - +0.3 1,767,033 - +0.7 1,767,043 - +1.2 1,767,115 + +0.9 1,767,250 + ydiK b1688 0.14 +0.1 1,767,471 - ydiK b1688 0.34 +0.8 1,767,542 + ydiK b1688 0.40 +1.3 1,767,589 + ydiK b1688 0.44 -1.5 1,767,631 - ydiK b1688 0.48 -0.4 1,767,747 + ydiK b1688 0.58 +0.1 1,767,784 - ydiK b1688 0.62 +0.0 1,767,814 - ydiK b1688 0.64 -0.3 1,767,850 - ydiK b1688 0.68 +0.3 1,767,962 - ydiK b1688 0.78 -2.3 1,768,086 - ydiK b1688 0.89 -1.9 1,768,225 + -1.3 1,768,225 + +1.4 1,768,227 + +0.1 1,768,290 - +0.3 1,768,307 + -0.3 1,768,307 + +0.9 1,768,312 - +1.1 1,768,382 + +0.2 1,768,390 - +0.2 1,768,466 - rprA b4431 0.66 -0.5 1,768,471 - rprA b4431 0.71 -0.3 1,768,479 + rprA b4431 0.78 -1.1 1,768,516 + +1.2 1,768,551 + +1.1 1,768,559 - -0.3 1,768,561 + +1.2 1,768,561 + +0.2 1,768,569 + +1.3 1,768,569 + -2.3 1,768,569 - -0.6 1,768,571 + +0.4 1,768,654 + +1.5 1,768,661 - -0.1 1,768,685 + b1689 b1689 0.13 +0.1 1,768,714 - b1689 b1689 0.21 +0.0 1,768,720 + b1689 b1689 0.23 -0.0 1,768,765 + b1689 b1689 0.35 -1.4 1,768,765 + b1689 b1689 0.35 -0.1 1,768,781 - b1689 b1689 0.40 -0.5 1,768,788 + b1689 b1689 0.42 +0.1 1,768,788 + b1689 b1689 0.42 +0.8 1,768,905 + b1689 b1689 0.75 -0.1 1,768,905 + b1689 b1689 0.75 +1.7 1,768,905 + b1689 b1689 0.75 +1.3 1,768,913 - b1689 b1689 0.77 -2.0 1,768,976 + -0.8 1,769,159 - -0.7 1,769,186 - -2.3 1,769,198 + -2.7
Or see this region's nucleotide sequence