Strain Fitness in Escherichia coli BW25113 around b1075

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgA and flgB are separated by 154 nucleotidesflgB and flgC are separated by 3 nucleotidesflgC and flgD are separated by 11 nucleotidesflgD and flgE are separated by 24 nucleotides b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086 flgA b1073: flgB - flagellar basal-body rod protein B (NCBI), at 1,130,241 to 1,130,657 flgB b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065 flgC b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772 flgD b1076: flgE - flagellar hook protein E (NCBI), at 1,131,797 to 1,133,005 flgE Position (kb) 1131 1132Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1130.097 kb on + strandat 1130.110 kb on + strandat 1130.161 kb on + strandat 1130.236 kb on - strandat 1130.238 kb on - strandat 1130.238 kb on - strandat 1130.270 kb on - strandat 1130.352 kb on - strand, within flgBat 1130.352 kb on - strand, within flgBat 1130.411 kb on + strand, within flgBat 1130.411 kb on + strand, within flgBat 1130.456 kb on - strand, within flgBat 1130.456 kb on - strand, within flgBat 1130.513 kb on - strand, within flgBat 1130.570 kb on - strand, within flgBat 1130.693 kb on - strandat 1130.701 kb on + strandat 1131.014 kb on + strand, within flgCat 1131.014 kb on + strand, within flgCat 1131.054 kb on - strandat 1131.111 kb on + strandat 1131.325 kb on + strand, within flgDat 1131.362 kb on - strand, within flgDat 1131.362 kb on - strand, within flgDat 1131.586 kb on + strand, within flgDat 1131.594 kb on - strand, within flgDat 1131.794 kb on + strandat 1131.794 kb on + strandat 1131.846 kb on - strandat 1131.996 kb on - strand, within flgEat 1131.996 kb on - strand, within flgEat 1132.002 kb on - strand, within flgEat 1132.010 kb on + strand, within flgEat 1132.176 kb on - strand, within flgEat 1132.283 kb on - strand, within flgEat 1132.366 kb on + strand, within flgEat 1132.366 kb on + strand, within flgEat 1132.384 kb on + strand, within flgEat 1132.423 kb on + strand, within flgEat 1132.463 kb on - strand, within flgEat 1132.463 kb on - strand, within flgEat 1132.510 kb on - strand, within flgEat 1132.551 kb on + strand, within flgEat 1132.559 kb on - strand, within flgEat 1132.559 kb on - strand, within flgEat 1132.574 kb on - strand, within flgEat 1132.584 kb on - strand, within flgEat 1132.605 kb on - strand, within flgEat 1132.625 kb on - strand, within flgEat 1132.625 kb on - strand, within flgEat 1132.694 kb on - strand, within flgE

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,130,097 + -0.2
1,130,110 + +1.6
1,130,161 + +2.6
1,130,236 - -2.0
1,130,238 - -1.4
1,130,238 - -1.5
1,130,270 - +0.7
1,130,352 - flgB b1073 0.27 -2.0
1,130,352 - flgB b1073 0.27 +2.6
1,130,411 + flgB b1073 0.41 -1.0
1,130,411 + flgB b1073 0.41 -0.1
1,130,456 - flgB b1073 0.52 -2.3
1,130,456 - flgB b1073 0.52 +1.1
1,130,513 - flgB b1073 0.65 -3.5
1,130,570 - flgB b1073 0.79 -2.3
1,130,693 - -1.9
1,130,701 + +0.3
1,131,014 + flgC b1074 0.87 -1.0
1,131,014 + flgC b1074 0.87 -0.9
1,131,054 - -1.5
1,131,111 + +2.3
1,131,325 + flgD b1075 0.36 -0.8
1,131,362 - flgD b1075 0.41 -2.1
1,131,362 - flgD b1075 0.41 -3.0
1,131,586 + flgD b1075 0.73 -2.8
1,131,594 - flgD b1075 0.74 -1.1
1,131,794 + +0.0
1,131,794 + -2.1
1,131,846 - -2.7
1,131,996 - flgE b1076 0.16 +0.0
1,131,996 - flgE b1076 0.16 +0.9
1,132,002 - flgE b1076 0.17 -0.9
1,132,010 + flgE b1076 0.18 -1.2
1,132,176 - flgE b1076 0.31 -2.4
1,132,283 - flgE b1076 0.40 -0.9
1,132,366 + flgE b1076 0.47 +0.9
1,132,366 + flgE b1076 0.47 -2.3
1,132,384 + flgE b1076 0.49 +2.7
1,132,423 + flgE b1076 0.52 -0.3
1,132,463 - flgE b1076 0.55 +0.0
1,132,463 - flgE b1076 0.55 -2.4
1,132,510 - flgE b1076 0.59 -1.4
1,132,551 + flgE b1076 0.62 -1.5
1,132,559 - flgE b1076 0.63 -0.8
1,132,559 - flgE b1076 0.63 -0.2
1,132,574 - flgE b1076 0.64 -1.4
1,132,584 - flgE b1076 0.65 +0.0
1,132,605 - flgE b1076 0.67 -1.8
1,132,625 - flgE b1076 0.68 +1.8
1,132,625 - flgE b1076 0.68 -1.0
1,132,694 - flgE b1076 0.74 -1.6

Or see this region's nucleotide sequence