Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rimJ and yceH are separated by 10 nucleotides yceH and mviM are separated by 1 nucleotides mviM and mviN are separated by 109 nucleotides
b1066: rimJ - ribosomal-protein-S5-alanine N-acetyltransferase (NCBI), at 1,124,785 to 1,125,369
rimJ
b1067: yceH - hypothetical protein (NCBI), at 1,125,380 to 1,126,027
yceH
b1068: mviM - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 1,126,029 to 1,126,952
mviM
b1069: mviN - predicted inner membrane protein (NCBI), at 1,127,062 to 1,128,597
mviN
Position (kb) 1126
1127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1125.088 kb on + strand, within rimJ at 1125.142 kb on - strand, within rimJ at 1125.142 kb on - strand, within rimJ at 1125.157 kb on - strand, within rimJ at 1125.157 kb on - strand, within rimJ at 1125.169 kb on + strand, within rimJ at 1125.173 kb on + strand, within rimJ at 1125.181 kb on - strand, within rimJ at 1125.184 kb on - strand, within rimJ at 1125.236 kb on - strand, within rimJ at 1125.289 kb on - strand, within rimJ at 1125.289 kb on - strand, within rimJ at 1125.352 kb on - strand at 1125.407 kb on - strand at 1125.468 kb on + strand at 1125.586 kb on - strand, within yceH at 1125.605 kb on - strand, within yceH at 1125.725 kb on - strand, within yceH at 1125.725 kb on - strand, within yceH at 1125.862 kb on + strand, within yceH at 1125.937 kb on - strand, within yceH at 1126.116 kb on + strand at 1126.190 kb on - strand, within mviM at 1126.238 kb on + strand, within mviM at 1126.238 kb on + strand, within mviM at 1126.248 kb on + strand, within mviM at 1126.277 kb on + strand, within mviM at 1126.341 kb on - strand, within mviM at 1126.341 kb on - strand, within mviM at 1126.341 kb on - strand, within mviM at 1126.409 kb on + strand, within mviM at 1126.409 kb on + strand, within mviM at 1126.409 kb on + strand, within mviM at 1126.409 kb on + strand, within mviM at 1126.417 kb on - strand, within mviM at 1126.417 kb on - strand, within mviM at 1126.417 kb on - strand, within mviM at 1126.418 kb on - strand, within mviM at 1126.418 kb on - strand, within mviM at 1126.461 kb on + strand, within mviM at 1126.478 kb on - strand, within mviM at 1126.478 kb on - strand, within mviM at 1126.495 kb on + strand, within mviM at 1126.509 kb on + strand, within mviM at 1126.595 kb on - strand, within mviM at 1126.668 kb on - strand, within mviM at 1126.730 kb on - strand, within mviM at 1126.730 kb on - strand, within mviM at 1126.766 kb on + strand, within mviM at 1126.777 kb on - strand, within mviM at 1126.815 kb on - strand, within mviM at 1126.925 kb on + strand at 1126.925 kb on + strand at 1126.926 kb on + strand at 1126.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,125,088 + rimJ b1066 0.52 -0.3 1,125,142 - rimJ b1066 0.61 +0.2 1,125,142 - rimJ b1066 0.61 +0.6 1,125,157 - rimJ b1066 0.64 -0.7 1,125,157 - rimJ b1066 0.64 -0.7 1,125,169 + rimJ b1066 0.66 +0.6 1,125,173 + rimJ b1066 0.66 -3.1 1,125,181 - rimJ b1066 0.68 -1.4 1,125,184 - rimJ b1066 0.68 -2.9 1,125,236 - rimJ b1066 0.77 +0.2 1,125,289 - rimJ b1066 0.86 +0.2 1,125,289 - rimJ b1066 0.86 -0.5 1,125,352 - -0.6 1,125,407 - -2.0 1,125,468 + +3.2 1,125,586 - yceH b1067 0.32 +1.2 1,125,605 - yceH b1067 0.35 +2.2 1,125,725 - yceH b1067 0.53 -2.3 1,125,725 - yceH b1067 0.53 -0.6 1,125,862 + yceH b1067 0.74 -0.7 1,125,937 - yceH b1067 0.86 -0.1 1,126,116 + +0.8 1,126,190 - mviM b1068 0.17 -1.2 1,126,238 + mviM b1068 0.23 +0.3 1,126,238 + mviM b1068 0.23 -1.1 1,126,248 + mviM b1068 0.24 -0.4 1,126,277 + mviM b1068 0.27 +2.4 1,126,341 - mviM b1068 0.34 -0.6 1,126,341 - mviM b1068 0.34 +0.6 1,126,341 - mviM b1068 0.34 -1.1 1,126,409 + mviM b1068 0.41 -1.6 1,126,409 + mviM b1068 0.41 +1.1 1,126,409 + mviM b1068 0.41 -0.5 1,126,409 + mviM b1068 0.41 +0.3 1,126,417 - mviM b1068 0.42 +0.3 1,126,417 - mviM b1068 0.42 +0.1 1,126,417 - mviM b1068 0.42 -0.6 1,126,418 - mviM b1068 0.42 +1.0 1,126,418 - mviM b1068 0.42 +0.7 1,126,461 + mviM b1068 0.47 -1.8 1,126,478 - mviM b1068 0.49 -0.1 1,126,478 - mviM b1068 0.49 -1.9 1,126,495 + mviM b1068 0.50 +0.1 1,126,509 + mviM b1068 0.52 +3.2 1,126,595 - mviM b1068 0.61 +0.3 1,126,668 - mviM b1068 0.69 -0.5 1,126,730 - mviM b1068 0.76 -0.5 1,126,730 - mviM b1068 0.76 +0.2 1,126,766 + mviM b1068 0.80 -0.9 1,126,777 - mviM b1068 0.81 -0.8 1,126,815 - mviM b1068 0.85 -0.7 1,126,925 + +1.1 1,126,925 + +1.1 1,126,926 + -1.0 1,126,956 + +0.3
Or see this region's nucleotide sequence