Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybfD and ybgA are separated by 147 nucleotides ybgA and phr overlap by 4 nucleotides phr and ybgH are separated by 149 nucleotides
b0706: ybfD - hypothetical protein (NCBI), at 737,315 to 738,076
ybfD
b0707: ybgA - hypothetical protein (NCBI), at 738,224 to 738,733
ybgA
b0708: phr - deoxyribodipyrimidine photolyase, FAD-binding (NCBI), at 738,730 to 740,148
phr
b0709: ybgH - predicted transporter (NCBI), at 740,298 to 741,779
ybgH
Position (kb) 738
739
740
741 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 737.767 kb on + strand, within ybfD at 737.777 kb on + strand, within ybfD at 737.977 kb on - strand, within ybfD at 737.979 kb on - strand, within ybfD at 738.012 kb on - strand at 738.032 kb on - strand at 738.120 kb on - strand at 738.199 kb on - strand at 738.200 kb on + strand at 738.200 kb on + strand at 738.208 kb on - strand at 738.208 kb on - strand at 738.211 kb on + strand at 738.251 kb on + strand at 738.252 kb on + strand at 738.392 kb on - strand, within ybgA at 738.392 kb on - strand, within ybgA at 738.435 kb on + strand, within ybgA at 738.443 kb on - strand, within ybgA at 738.531 kb on - strand, within ybgA at 738.710 kb on - strand at 738.717 kb on + strand at 738.717 kb on + strand at 738.759 kb on + strand at 738.787 kb on - strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.831 kb on + strand at 738.837 kb on + strand at 738.844 kb on - strand at 738.844 kb on - strand at 738.844 kb on - strand at 739.092 kb on + strand, within phr at 739.122 kb on - strand, within phr at 739.124 kb on + strand, within phr at 739.322 kb on - strand, within phr at 739.415 kb on + strand, within phr at 739.415 kb on + strand, within phr at 739.475 kb on - strand, within phr at 739.648 kb on + strand, within phr at 739.648 kb on + strand, within phr at 739.648 kb on - strand, within phr at 739.648 kb on - strand, within phr at 739.720 kb on + strand, within phr at 739.765 kb on - strand, within phr at 740.129 kb on - strand at 740.287 kb on + strand at 740.366 kb on + strand at 740.534 kb on - strand, within ybgH at 740.728 kb on - strand, within ybgH at 740.728 kb on - strand, within ybgH at 740.753 kb on + strand, within ybgH at 740.756 kb on - strand, within ybgH at 740.813 kb on + strand, within ybgH at 740.813 kb on + strand, within ybgH at 740.813 kb on + strand, within ybgH at 740.821 kb on - strand, within ybgH at 740.821 kb on - strand, within ybgH at 740.924 kb on + strand, within ybgH at 740.932 kb on - strand, within ybgH at 741.037 kb on - strand, within ybgH at 741.041 kb on - strand, within ybgH at 741.041 kb on - strand, within ybgH at 741.046 kb on - strand, within ybgH at 741.046 kb on - strand, within ybgH at 741.071 kb on - strand, within ybgH at 741.071 kb on - strand, within ybgH at 741.071 kb on - strand, within ybgH at 741.082 kb on + strand, within ybgH at 741.082 kb on + strand, within ybgH at 741.090 kb on - strand, within ybgH at 741.092 kb on + strand, within ybgH at 741.092 kb on + strand, within ybgH at 741.100 kb on - strand, within ybgH at 741.100 kb on - strand, within ybgH at 741.102 kb on + strand, within ybgH at 741.104 kb on - strand, within ybgH
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 737,767 + ybfD b0706 0.59 -0.5 737,777 + ybfD b0706 0.61 -1.2 737,977 - ybfD b0706 0.87 +1.4 737,979 - ybfD b0706 0.87 +1.8 738,012 - -0.4 738,032 - +0.4 738,120 - -0.2 738,199 - -0.3 738,200 + -1.8 738,200 + +1.2 738,208 - +0.1 738,208 - +0.0 738,211 + +1.1 738,251 + -1.2 738,252 + -0.9 738,392 - ybgA b0707 0.33 +0.8 738,392 - ybgA b0707 0.33 -0.0 738,435 + ybgA b0707 0.41 +0.1 738,443 - ybgA b0707 0.43 +1.5 738,531 - ybgA b0707 0.60 +1.4 738,710 - +1.2 738,717 + +0.8 738,717 + -0.0 738,759 + +1.5 738,787 - +0.1 738,831 + +0.2 738,831 + -1.6 738,831 + -1.2 738,831 + -2.7 738,837 + +1.6 738,844 - +0.4 738,844 - -0.5 738,844 - +0.3 739,092 + phr b0708 0.26 +0.9 739,122 - phr b0708 0.28 -1.2 739,124 + phr b0708 0.28 +0.1 739,322 - phr b0708 0.42 -1.1 739,415 + phr b0708 0.48 +0.4 739,415 + phr b0708 0.48 +0.9 739,475 - phr b0708 0.53 +0.9 739,648 + phr b0708 0.65 +0.8 739,648 + phr b0708 0.65 +0.2 739,648 - phr b0708 0.65 -2.8 739,648 - phr b0708 0.65 +2.4 739,720 + phr b0708 0.70 -0.9 739,765 - phr b0708 0.73 -0.2 740,129 - +1.1 740,287 + -0.4 740,366 + +1.5 740,534 - ybgH b0709 0.16 +0.1 740,728 - ybgH b0709 0.29 +1.2 740,728 - ybgH b0709 0.29 -0.2 740,753 + ybgH b0709 0.31 +1.2 740,756 - ybgH b0709 0.31 +0.3 740,813 + ybgH b0709 0.35 +1.2 740,813 + ybgH b0709 0.35 +0.8 740,813 + ybgH b0709 0.35 +0.4 740,821 - ybgH b0709 0.35 +1.3 740,821 - ybgH b0709 0.35 -2.0 740,924 + ybgH b0709 0.42 +0.7 740,932 - ybgH b0709 0.43 +0.7 741,037 - ybgH b0709 0.50 +1.8 741,041 - ybgH b0709 0.50 +0.2 741,041 - ybgH b0709 0.50 -1.1 741,046 - ybgH b0709 0.50 +2.2 741,046 - ybgH b0709 0.50 +1.1 741,071 - ybgH b0709 0.52 +0.1 741,071 - ybgH b0709 0.52 +1.1 741,071 - ybgH b0709 0.52 -0.0 741,082 + ybgH b0709 0.53 +0.5 741,082 + ybgH b0709 0.53 -0.5 741,090 - ybgH b0709 0.53 -0.3 741,092 + ybgH b0709 0.54 -0.2 741,092 + ybgH b0709 0.54 -0.8 741,100 - ybgH b0709 0.54 +1.1 741,100 - ybgH b0709 0.54 -0.2 741,102 + ybgH b0709 0.54 +1.0 741,104 - ybgH b0709 0.54 +0.0
Or see this region's nucleotide sequence