Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citF and citE are separated by 10 nucleotides citE and citD overlap by 4 nucleotides citD and citC are separated by 14 nucleotides
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079
citC
Position (kb) 649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 648.810 kb on - strand at 649.122 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.327 kb on - strand, within citE at 649.479 kb on + strand, within citE at 649.540 kb on - strand, within citE at 649.569 kb on + strand, within citE at 649.595 kb on - strand, within citE at 649.595 kb on - strand, within citE at 649.607 kb on + strand, within citE at 649.642 kb on + strand at 649.665 kb on - strand at 649.828 kb on - strand, within citD at 649.828 kb on - strand, within citD at 649.831 kb on - strand, within citD at 649.947 kb on - strand, within citD at 650.199 kb on + strand, within citC at 650.221 kb on + strand, within citC at 650.310 kb on - strand, within citC at 650.326 kb on + strand, within citC at 650.332 kb on - strand, within citC at 650.332 kb on - strand at 650.456 kb on - strand, within citC at 650.577 kb on + strand, within citC at 650.733 kb on - strand, within citC at 650.794 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.816 kb on - strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.826 kb on - strand, within citC at 650.858 kb on + strand, within citC at 650.860 kb on - strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on - strand, within citC at 650.955 kb on + strand, within citC at 650.955 kb on + strand, within citC at 650.962 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.978 kb on + strand at 650.978 kb on + strand at 650.988 kb on + strand at 650.988 kb on + strand at 650.992 kb on + strand at 650.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 648,810 - -0.1 649,122 - citE b0616 0.35 -0.5 649,238 - citE b0616 0.48 -1.8 649,238 - citE b0616 0.48 -1.4 649,327 - citE b0616 0.57 +0.7 649,479 + citE b0616 0.74 -0.3 649,540 - citE b0616 0.81 +0.2 649,569 + citE b0616 0.84 -0.9 649,595 - citE b0616 0.87 -0.2 649,595 - citE b0616 0.87 -1.4 649,607 + citE b0616 0.88 +0.5 649,642 + +0.6 649,665 - +0.6 649,828 - citD b0617 0.40 +2.5 649,828 - citD b0617 0.40 +1.3 649,831 - citD b0617 0.41 +1.4 649,947 - citD b0617 0.80 +1.3 650,199 + citC b0618 0.17 -1.2 650,221 + citC b0618 0.19 +1.7 650,310 - citC b0618 0.27 -0.0 650,326 + citC b0618 0.29 +0.8 650,332 - citC b0618 0.29 -1.0 650,332 - +3.3 650,456 - citC b0618 0.41 +0.6 650,577 + citC b0618 0.53 -0.4 650,733 - citC b0618 0.67 +0.0 650,794 + citC b0618 0.73 -1.3 650,808 + citC b0618 0.74 +0.2 650,808 + citC b0618 0.74 -0.1 650,808 + citC b0618 0.74 +0.8 650,816 - citC b0618 0.75 +1.0 650,818 + citC b0618 0.75 -0.7 650,818 + citC b0618 0.75 +0.4 650,818 + citC b0618 0.75 +1.2 650,826 - citC b0618 0.76 -0.7 650,858 + citC b0618 0.79 -0.8 650,860 - citC b0618 0.79 +1.5 650,863 + citC b0618 0.80 -0.4 650,863 + citC b0618 0.80 -0.5 650,863 + citC b0618 0.80 -0.3 650,885 + citC b0618 0.82 -1.4 650,885 + citC b0618 0.82 +1.3 650,885 + citC b0618 0.82 +0.5 650,888 + citC b0618 0.82 +0.4 650,888 + citC b0618 0.82 -1.4 650,888 - citC b0618 0.82 -2.8 650,955 + citC b0618 0.88 -0.2 650,955 + citC b0618 0.88 -1.6 650,962 + citC b0618 0.89 -0.1 650,966 + citC b0618 0.89 +1.8 650,966 + citC b0618 0.89 -2.3 650,978 + -1.7 650,978 + -1.0 650,988 + +1.3 650,988 + +1.5 650,992 + -1.7 650,992 + -2.4
Or see this region's nucleotide sequence