Strain Fitness in Escherichia coli BW25113 around b4478

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
3,868,904	-	dgoT	b3691	0.34	-1.3
3,868,988	-	dgoT	b3691	0.41	+2.0
3,868,988	-	dgoT	b3691	0.41	+0.5
3,868,996	-	dgoT	b3691	0.41	+0.5
3,868,996	-	dgoT	b3691	0.41	-0.1
3,868,996	-	dgoT	b3691	0.41	-0.8
3,868,996	-	dgoT	b3691	0.41	-0.6
3,868,996	-	dgoT	b3691	0.41	+1.7
3,869,038	-	dgoT	b3691	0.45	-0.4
3,869,045	+	dgoT	b3691	0.45	-0.3
3,869,045	+	dgoT	b3691	0.45	+1.3
3,869,057	-	dgoT	b3691	0.46	-2.1
3,869,057	-	dgoT	b3691	0.46	+0.8
3,869,131	+	dgoT	b3691	0.52	+0.2
3,869,139	-	dgoT	b3691	0.52	-0.5
3,869,144	-	dgoT	b3691	0.53	-0.5
3,869,208	+	dgoT	b3691	0.58	+3.1
3,869,213	+	dgoT	b3691	0.58	-2.1
3,869,240	+	dgoT	b3691	0.60	+0.5
3,869,252	-	dgoT	b3691	0.61	+0.7
3,869,265	+	dgoT	b3691	0.62	+0.2
3,869,307	-	dgoT	b3691	0.65	-0.0
3,869,332	+	dgoT	b3691	0.67	+0.4
3,869,338	-	dgoT	b3691	0.68	-2.8
3,869,338	-	dgoT	b3691	0.68	-0.7
3,869,351	-	dgoT	b3691	0.69	-0.1
3,869,365	+	dgoT	b3691	0.70	+0.5
3,869,387	+	dgoT	b3691	0.72	-0.4
3,869,387	+	dgoT	b3691	0.72	-2.5
3,869,419	-	dgoT	b3691	0.74	+3.3
3,869,438	-	dgoT	b3691	0.76	+0.3
3,869,450	+	dgoT	b3691	0.76	-1.2
3,869,465	-	dgoT	b3691	0.78	+0.1
3,869,477	-	dgoT	b3691	0.79	-1.1
3,869,477	-	dgoT	b3691	0.79	-1.1
3,869,508	-	dgoT	b3691	0.81	-0.9
3,869,515	-	dgoT	b3691	0.82	-0.1
3,869,546	+	dgoT	b3691	0.84	+2.1
3,869,555	-	dgoT	b3691	0.85	-0.4
3,869,597	+	dgoT	b3691	0.88	-1.1
3,869,614	-	dgoT	b3691	0.89	+3.3
3,869,614	-	dgoT	b3691	0.89	-1.0
3,869,630	-				-0.1
3,869,630	-				+0.5
3,869,702	+				-0.5
3,869,712	-				+0.0
3,869,751	-				-1.5
3,869,764	+				+2.8
3,869,810	-				-0.7
3,869,810	-				+1.9
3,869,864	-				+0.1
3,869,870	+				+0.6
3,869,870	+				+0.5
3,869,914	+				+0.9
3,869,914	+				-0.8
3,869,947	+				-1.4
3,869,947	+				-2.4
3,869,953	+				+0.4
3,869,983	-				-0.4
3,869,989	-	dgoD	b4478	0.10	-0.3
3,869,989	-	dgoD	b4478	0.10	-0.3
3,870,028	-	dgoD	b4478	0.13	-1.1
3,870,113	-	dgoD	b4478	0.21	-1.0
3,870,113	-	dgoD	b4478	0.21	+0.7
3,870,373	-	dgoD	b4478	0.44	-0.2
3,870,404	-	dgoD	b4478	0.46	-1.9
3,870,470	+	dgoD	b4478	0.52	+1.3
3,870,497	+	dgoD	b4478	0.54	+0.5
3,870,525	+	dgoD	b4478	0.57	+0.6
3,870,532	-	dgoD	b4478	0.57	-0.9
3,870,532	-	dgoD	b4478	0.57	-1.2
3,870,551	+	dgoD	b4478	0.59	-1.2
3,870,551	+	dgoD	b4478	0.59	+0.3
3,870,574	+	dgoD	b4478	0.61	+0.4
3,870,582	-	dgoD	b4478	0.62	-0.3
3,870,582	-	dgoD	b4478	0.62	+0.2
3,870,582	-	dgoD	b4478	0.62	-0.2
3,870,755	+	dgoD	b4478	0.77	+0.6
3,870,755	+	dgoD	b4478	0.77	+0.5
3,870,758	-	dgoD	b4478	0.77	-0.6
3,870,826	-	dgoD	b4478	0.83	+0.3
3,870,826	-	dgoD	b4478	0.83	-1.4
3,870,826	-	dgoD	b4478	0.83	+0.6
3,870,939	-				-1.1
3,870,995	-				-2.0
3,871,028	+				-0.8
3,871,028	+				-0.4
3,871,028	+				-0.4
3,871,036	-				+0.1
3,871,040	+				+1.7
3,871,040	+				-0.2
3,871,177	+	dgoA	b4477	0.26	+0.4
3,871,321	+	dgoA	b4477	0.49	-0.7
3,871,321	+	dgoA	b4477	0.49	+1.6
3,871,332	+	dgoA	b4477	0.51	-0.5
3,871,332	+	dgoA	b4477	0.51	+0.4
3,871,342	-	dgoA	b4477	0.52	-1.0
3,871,352	-	dgoA	b4477	0.54	-1.3
3,871,352	-	dgoA	b4477	0.54	+1.6
3,871,359	-	dgoA	b4477	0.55	-0.7
3,871,586	-				+2.3
3,871,592	-				+1.2
3,871,645	+				+0.6
3,871,646	+				-1.4
3,871,664	-				-0.5
3,871,738	+	dgoK	b3693	0.14	-0.2
3,871,761	-	dgoK	b3693	0.16	+0.1
3,871,762	+	dgoK	b3693	0.16	+0.5
3,871,763	-	dgoK	b3693	0.16	+0.5
3,871,763	-	dgoK	b3693	0.16	-1.5
3,871,774	-	dgoK	b3693	0.18	+0.3
3,871,774	-	dgoK	b3693	0.18	+0.1
3,871,784	-	dgoK	b3693	0.19	+0.7
3,871,784	-	dgoK	b3693	0.19	+2.1
3,871,799	-	dgoK	b3693	0.20	+1.0
3,871,825	-	dgoK	b3693	0.23	+0.8
3,871,835	+	dgoK	b3693	0.25	+0.3
3,871,835	+	dgoK	b3693	0.25	-0.9
3,871,848	-	dgoK	b3693	0.26	-1.2
3,871,848	-	dgoK	b3693	0.26	+1.4
3,871,917	-	dgoK	b3693	0.34	+0.2
3,871,940	-	dgoK	b3693	0.37	+0.3