Strain Fitness in Escherichia coli BW25113 around b2164

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
2,253,146	+				-1.0
2,253,146	+				-0.5
2,253,146	+				+1.4
2,253,298	+				+1.3
2,253,334	-				-1.0
2,253,411	-				-0.2
2,253,411	-				+0.0
2,253,415	+				-0.8
2,253,436	-				+0.6
2,253,436	-				-1.5
2,253,462	-	yeiL	b2163	0.13	-1.1
2,253,472	-	yeiL	b2163	0.14	-0.7
2,253,493	+	yeiL	b2163	0.18	+0.6
2,253,503	+	yeiL	b2163	0.19	-2.5
2,253,503	+	yeiL	b2163	0.19	-1.0
2,253,526	+	yeiL	b2163	0.23	+0.4
2,253,543	-	yeiL	b2163	0.25	+0.1
2,253,611	-	yeiL	b2163	0.35	+1.3
2,253,613	+	yeiL	b2163	0.36	-1.6
2,253,613	+	yeiL	b2163	0.36	+0.3
2,253,620	-	yeiL	b2163	0.37	+0.8
2,253,620	-	yeiL	b2163	0.37	+0.1
2,253,620	-	yeiL	b2163	0.37	-2.3
2,253,621	+	yeiL	b2163	0.37	+0.7
2,253,636	+	yeiL	b2163	0.39	-1.8
2,253,637	-	yeiL	b2163	0.39	+1.4
2,253,646	+	yeiL	b2163	0.41	+1.7
2,253,678	+	yeiL	b2163	0.46	+0.8
2,253,836	-	yeiL	b2163	0.70	-1.9
2,253,838	-	yeiL	b2163	0.70	+0.1
2,253,902	-	yeiL	b2163	0.80	-0.3
2,253,911	-	yeiL	b2163	0.81	-2.5
2,253,921	-	yeiL	b2163	0.82	-1.3
2,253,921	-	yeiL	b2163	0.82	+0.7
2,253,984	+				-1.9
2,253,984	-				+0.7
2,253,986	-				+0.6
2,253,986	-				-1.0
2,253,986	-				+0.9
2,253,986	-				-0.6
2,253,996	+				+0.4
2,254,068	-				-0.5
2,254,068	-				+2.6
2,254,088	-				+0.1
2,254,088	-				+0.2
2,254,089	-				-1.6
2,254,100	-				+3.2
2,254,100	-				+3.3
2,254,109	+				-1.4
2,254,113	-				+0.3
2,254,117	-				-0.4
2,254,117	-				-0.5
2,254,117	-				+0.1
2,254,125	-				-0.4
2,254,144	-				-1.5
2,254,144	-				-0.3
2,254,147	-				+0.8
2,254,154	-				+0.0
2,254,158	-				+1.1
2,254,158	-				-0.1
2,254,158	-				+0.7
2,254,173	-				-0.5
2,254,263	-	yeiM	b2164	0.12	-0.4
2,254,297	-	yeiM	b2164	0.15	+1.9
2,254,417	+	yeiM	b2164	0.25	-0.5
2,254,430	-	yeiM	b2164	0.26	+2.0
2,254,430	-	yeiM	b2164	0.26	+0.7
2,254,457	-	yeiM	b2164	0.28	+1.2
2,254,457	-	yeiM	b2164	0.28	-0.1
2,254,533	-	yeiM	b2164	0.34	+1.4
2,254,741	+	yeiM	b2164	0.51	+0.7
2,254,766	-	yeiM	b2164	0.53	+1.7
2,254,820	-	yeiM	b2164	0.57	-1.7
2,254,820	-	yeiM	b2164	0.57	-1.1
2,255,008	-	yeiM	b2164	0.72	-0.3
2,255,008	-	yeiM	b2164	0.72	+0.7
2,255,016	-	yeiM	b2164	0.73	+0.4
2,255,016	-	yeiM	b2164	0.73	-0.0
2,255,021	-	yeiM	b2164	0.73	-1.5
2,255,157	-	yeiM	b2164	0.84	+1.2
2,255,157	-	yeiM	b2164	0.84	+1.4
2,255,211	+	yeiM	b2164	0.88	+0.8
2,255,211	+	yeiM	b2164	0.88	-1.6
2,255,327	-				+1.0
2,255,331	+				+0.0
2,255,434	+				-0.7
2,255,474	-				+0.4
2,255,639	+	yeiN	b2165	0.20	+0.2
2,255,688	+	yeiN	b2165	0.25	-0.7
2,255,702	-	yeiN	b2165	0.27	-0.2
2,255,777	+	yeiN	b2165	0.35	-0.0
2,255,805	+	yeiN	b2165	0.38	-1.0
2,255,831	-	yeiN	b2165	0.40	-0.3
2,255,833	+	yeiN	b2165	0.41	-0.6
2,255,833	+	yeiN	b2165	0.41	-2.5
2,255,935	+	yeiN	b2165	0.52	+0.5
2,255,936	+	yeiN	b2165	0.52	-0.9
2,255,936	+	yeiN	b2165	0.52	+1.1
2,255,961	+	yeiN	b2165	0.54	+0.2
2,255,966	+	yeiN	b2165	0.55	-0.1
2,255,974	-	yeiN	b2165	0.56	-2.0
2,255,974	-	yeiN	b2165	0.56	-2.0
2,255,979	-	yeiN	b2165	0.56	-2.2
2,256,048	+	yeiN	b2165	0.64	+0.4
2,256,048	+	yeiN	b2165	0.64	+0.4
2,256,051	-	yeiN	b2165	0.64	-0.0
2,256,051	-	yeiN	b2165	0.64	-0.5
2,256,098	-	yeiN	b2165	0.69	+1.2
2,256,120	+	yeiN	b2165	0.71	+1.5
2,256,120	+	yeiN	b2165	0.71	-0.7
2,256,128	+	yeiN	b2165	0.72	-0.8
2,256,135	-	yeiN	b2165	0.73	-2.4
2,256,136	-	yeiN	b2165	0.73	-1.0