Strain Fitness in Escherichia coli BW25113 around b1942

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliI and fliJ are separated by 18 nucleotidesfliJ and fliK overlap by 4 nucleotides b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951 fliI b1942: fliJ - flagellar biosynthesis chaperone (NCBI), at 2,015,970 to 2,016,413 fliJ b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537 fliK Position (kb) 2015 2016 2017Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2015.057 kb on - strand, within fliIat 2015.057 kb on - strandat 2015.259 kb on - strand, within fliIat 2015.261 kb on - strand, within fliIat 2015.261 kb on - strand, within fliIat 2015.499 kb on - strand, within fliIat 2015.549 kb on + strand, within fliIat 2015.549 kb on + strand, within fliIat 2015.560 kb on + strand, within fliIat 2015.631 kb on + strand, within fliIat 2015.685 kb on - strand, within fliIat 2015.685 kb on - strand, within fliIat 2015.701 kb on + strandat 2015.701 kb on + strand, within fliIat 2015.847 kb on - strandat 2015.847 kb on - strandat 2016.216 kb on - strand, within fliJat 2016.216 kb on - strand, within fliJat 2016.658 kb on - strand, within fliKat 2016.927 kb on - strand, within fliKat 2016.957 kb on - strand, within fliKat 2017.027 kb on + strand, within fliKat 2017.068 kb on - strand, within fliKat 2017.076 kb on + strand, within fliKat 2017.076 kb on + strand, within fliKat 2017.080 kb on - strand, within fliKat 2017.116 kb on + strand, within fliKat 2017.182 kb on + strand, within fliKat 2017.207 kb on + strand, within fliKat 2017.207 kb on + strand, within fliKat 2017.267 kb on + strand, within fliKat 2017.323 kb on + strand, within fliK

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,015,057 - fliI b1941 0.35 -0.1
2,015,057 - +2.9
2,015,259 - fliI b1941 0.50 -2.9
2,015,261 - fliI b1941 0.50 -1.6
2,015,261 - fliI b1941 0.50 -0.7
2,015,499 - fliI b1941 0.67 -2.2
2,015,549 + fliI b1941 0.71 +0.5
2,015,549 + fliI b1941 0.71 +0.8
2,015,560 + fliI b1941 0.71 -2.6
2,015,631 + fliI b1941 0.77 -2.4
2,015,685 - fliI b1941 0.81 -3.3
2,015,685 - fliI b1941 0.81 -2.1
2,015,701 + +3.6
2,015,701 + fliI b1941 0.82 -2.1
2,015,847 - -0.8
2,015,847 - -2.7
2,016,216 - fliJ b1942 0.55 +3.1
2,016,216 - fliJ b1942 0.55 +0.7
2,016,658 - fliK b1943 0.22 -3.4
2,016,927 - fliK b1943 0.46 +0.8
2,016,957 - fliK b1943 0.48 -3.7
2,017,027 + fliK b1943 0.55 +1.0
2,017,068 - fliK b1943 0.58 -2.3
2,017,076 + fliK b1943 0.59 +0.7
2,017,076 + fliK b1943 0.59 -3.3
2,017,080 - fliK b1943 0.59 -3.8
2,017,116 + fliK b1943 0.63 +2.5
2,017,182 + fliK b1943 0.68 -1.6
2,017,207 + fliK b1943 0.71 +0.3
2,017,207 + fliK b1943 0.71 -0.3
2,017,267 + fliK b1943 0.76 -2.2
2,017,323 + fliK b1943 0.81 -0.9

Or see this region's nucleotide sequence