Strain Fitness in Escherichia coli BW25113 around b1637

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgst and pdxY are separated by 43 nucleotidespdxY and tyrS are separated by 58 nucleotidestyrS and pdxH are separated by 128 nucleotidespdxH and ydhA are separated by 58 nucleotides b1635: gst - glutathionine S-transferase (NCBI), at 1,712,401 to 1,713,006 gst b1636: pdxY - pyridoxine kinase (NCBI), at 1,713,050 to 1,713,913 pdxY b1637: tyrS - tyrosyl-tRNA synthetase (NCBI), at 1,713,972 to 1,715,246 tyrS b1638: pdxH - pyridoxamine 5'-phosphate oxidase (NCBI), at 1,715,375 to 1,716,031 pdxH b1639: ydhA - orf, hypothetical protein (VIMSS), at 1,716,090 to 1,716,419 ydhA Position (kb) 1713 1714 1715 1716Strain fitness (log2 ratio) -2 -1 0 1 2at 1713.214 kb on + strand, within pdxYat 1713.223 kb on + strand, within pdxYat 1713.223 kb on + strand, within pdxYat 1713.223 kb on + strand, within pdxYat 1713.352 kb on - strand, within pdxYat 1713.469 kb on + strand, within pdxYat 1713.546 kb on - strand, within pdxYat 1713.571 kb on + strand, within pdxYat 1713.571 kb on + strand, within pdxYat 1713.624 kb on - strand, within pdxYat 1713.889 kb on + strandat 1713.889 kb on + strandat 1713.913 kb on + strandat 1713.913 kb on + strandat 1715.267 kb on - strandat 1715.267 kb on - strandat 1716.201 kb on - strand, within ydhAat 1716.224 kb on + strand, within ydhAat 1716.226 kb on + strand, within ydhAat 1716.226 kb on + strand, within ydhA

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,713,214 + pdxY b1636 0.19 +1.0
1,713,223 + pdxY b1636 0.20 +0.7
1,713,223 + pdxY b1636 0.20 +1.8
1,713,223 + pdxY b1636 0.20 -0.7
1,713,352 - pdxY b1636 0.35 +0.5
1,713,469 + pdxY b1636 0.48 +1.1
1,713,546 - pdxY b1636 0.57 -0.1
1,713,571 + pdxY b1636 0.60 -0.3
1,713,571 + pdxY b1636 0.60 +0.8
1,713,624 - pdxY b1636 0.66 +0.9
1,713,889 + +1.5
1,713,889 + -1.4
1,713,913 + -0.0
1,713,913 + +2.1
1,715,267 - -2.7
1,715,267 - +0.5
1,716,201 - ydhA b1639 0.34 +1.8
1,716,224 + ydhA b1639 0.41 +2.0
1,716,226 + ydhA b1639 0.41 +1.0
1,716,226 + ydhA b1639 0.41 -0.0

Or see this region's nucleotide sequence