Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadA and pnuC are separated by 37 nucleotides pnuC and zitB overlap by 4 nucleotides
b0750: nadA - quinolinate synthetase (NCBI), at 781,308 to 782,351
nadA
b0751: pnuC - predicted nicotinamide mononucleotide transporter (NCBI), at 782,389 to 783,108
pnuC
b0752: zitB - zinc transporter ZitB (NCBI), at 783,105 to 784,046
zitB
Position (kb) 782
783
784 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 781.430 kb on - strand, within nadA at 781.430 kb on - strand, within nadA at 781.443 kb on + strand, within nadA at 781.456 kb on - strand, within nadA at 781.456 kb on - strand, within nadA at 781.456 kb on - strand, within nadA at 781.468 kb on + strand, within nadA at 781.708 kb on + strand, within nadA at 781.760 kb on - strand, within nadA at 781.847 kb on + strand, within nadA at 781.982 kb on - strand, within nadA at 782.171 kb on - strand, within nadA at 782.171 kb on - strand, within nadA at 782.226 kb on - strand, within nadA at 782.284 kb on + strand at 782.319 kb on + strand at 782.404 kb on + strand at 782.404 kb on + strand at 782.429 kb on - strand at 782.485 kb on + strand, within pnuC at 782.508 kb on - strand, within pnuC at 782.508 kb on - strand, within pnuC at 782.510 kb on + strand, within pnuC at 782.537 kb on - strand, within pnuC at 782.701 kb on + strand, within pnuC at 782.754 kb on + strand, within pnuC at 782.803 kb on + strand, within pnuC at 782.803 kb on + strand, within pnuC at 782.824 kb on + strand, within pnuC at 782.824 kb on + strand, within pnuC at 782.848 kb on - strand, within pnuC at 782.848 kb on - strand, within pnuC at 782.888 kb on - strand, within pnuC at 782.921 kb on + strand, within pnuC at 782.921 kb on + strand, within pnuC at 783.018 kb on - strand, within pnuC at 783.127 kb on - strand at 783.158 kb on + strand at 783.179 kb on - strand at 783.193 kb on - strand at 783.193 kb on - strand at 783.202 kb on + strand, within zitB at 783.202 kb on + strand, within zitB at 783.243 kb on - strand, within zitB at 783.270 kb on + strand, within zitB at 783.270 kb on + strand, within zitB at 783.317 kb on + strand, within zitB at 783.341 kb on + strand, within zitB at 783.352 kb on + strand, within zitB at 783.367 kb on + strand, within zitB at 783.392 kb on - strand, within zitB at 783.426 kb on + strand, within zitB at 783.445 kb on - strand, within zitB at 783.569 kb on - strand, within zitB at 783.763 kb on - strand, within zitB at 783.890 kb on + strand, within zitB at 784.014 kb on + strand at 784.014 kb on + strand at 784.014 kb on + strand at 784.014 kb on - strand at 784.014 kb on - strand at 784.022 kb on - strand at 784.036 kb on - strand at 784.036 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 781,430 - nadA b0750 0.12 +0.6 781,430 - nadA b0750 0.12 -0.1 781,443 + nadA b0750 0.13 +0.0 781,456 - nadA b0750 0.14 +0.4 781,456 - nadA b0750 0.14 +2.0 781,456 - nadA b0750 0.14 +2.2 781,468 + nadA b0750 0.15 +0.4 781,708 + nadA b0750 0.38 +0.8 781,760 - nadA b0750 0.43 -1.2 781,847 + nadA b0750 0.52 +0.2 781,982 - nadA b0750 0.65 -0.3 782,171 - nadA b0750 0.83 -1.2 782,171 - nadA b0750 0.83 -2.5 782,226 - nadA b0750 0.88 +0.1 782,284 + +1.1 782,319 + +0.1 782,404 + +2.2 782,404 + +0.3 782,429 - +1.6 782,485 + pnuC b0751 0.13 +1.2 782,508 - pnuC b0751 0.17 +0.0 782,508 - pnuC b0751 0.17 +0.1 782,510 + pnuC b0751 0.17 -1.7 782,537 - pnuC b0751 0.21 +0.7 782,701 + pnuC b0751 0.43 +0.6 782,754 + pnuC b0751 0.51 +0.7 782,803 + pnuC b0751 0.57 +1.3 782,803 + pnuC b0751 0.57 +5.9 782,824 + pnuC b0751 0.60 +1.1 782,824 + pnuC b0751 0.60 -3.4 782,848 - pnuC b0751 0.64 +1.6 782,848 - pnuC b0751 0.64 +0.6 782,888 - pnuC b0751 0.69 +1.1 782,921 + pnuC b0751 0.74 +2.1 782,921 + pnuC b0751 0.74 +0.6 783,018 - pnuC b0751 0.87 +1.3 783,127 - +0.1 783,158 + -1.2 783,179 - -0.8 783,193 - +0.3 783,193 - +0.9 783,202 + zitB b0752 0.10 +1.6 783,202 + zitB b0752 0.10 +1.8 783,243 - zitB b0752 0.15 +0.7 783,270 + zitB b0752 0.18 +2.4 783,270 + zitB b0752 0.18 +0.7 783,317 + zitB b0752 0.23 +1.0 783,341 + zitB b0752 0.25 -1.2 783,352 + zitB b0752 0.26 -0.4 783,367 + zitB b0752 0.28 +0.7 783,392 - zitB b0752 0.30 +0.4 783,426 + zitB b0752 0.34 +1.5 783,445 - zitB b0752 0.36 +0.2 783,569 - zitB b0752 0.49 -0.1 783,763 - zitB b0752 0.70 -0.1 783,890 + zitB b0752 0.83 -0.1 784,014 + -1.0 784,014 + -0.4 784,014 + -0.3 784,014 - -0.4 784,014 - +0.6 784,022 - -0.7 784,036 - -0.1 784,036 - -0.3
Or see this region's nucleotide sequence