Strain Fitness in Escherichia coli BW25113 around b3422

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
3,555,394	-	rtcB	b3421	0.42	-0.9
3,555,398	+	rtcB	b3421	0.43	+0.1
3,555,428	+	rtcB	b3421	0.45	+0.1
3,555,428	-	rtcB	b3421	0.45	+2.6
3,555,428	-	rtcB	b3421	0.45	+0.4
3,555,437	+	rtcB	b3421	0.46	-0.2
3,555,447	-	rtcB	b3421	0.47	+3.1
3,555,465	+	rtcB	b3421	0.48	-1.2
3,555,535	+	rtcB	b3421	0.54	+0.6
3,555,552	+	rtcB	b3421	0.55	+0.6
3,555,554	+	rtcB	b3421	0.55	-2.7
3,555,565	-	rtcB	b3421	0.56	-1.0
3,555,565	-	rtcB	b3421	0.56	-1.4
3,555,581	+	rtcB	b3421	0.58	+1.0
3,555,591	+	rtcB	b3421	0.58	-1.3
3,555,591	+	rtcB	b3421	0.58	+1.2
3,555,714	+	rtcB	b3421	0.68	-0.2
3,555,720	-	rtcB	b3421	0.69	-1.4
3,555,749	+	rtcB	b3421	0.71	+0.5
3,555,749	+	rtcB	b3421	0.71	+1.3
3,555,826	-	rtcB	b3421	0.78	+1.4
3,555,826	-	rtcB	b3421	0.78	+0.8
3,555,889	-	rtcB	b3421	0.83	+1.3
3,555,889	-	rtcB	b3421	0.83	-0.6
3,555,926	-	rtcB	b3421	0.86	-1.0
3,555,928	+	rtcB	b3421	0.86	+0.1
3,555,952	+	rtcB	b3421	0.88	-2.5
3,555,957	+	rtcB	b3421	0.88	+1.6
3,555,957	+	rtcB	b3421	0.88	+1.1
3,555,959	-	rtcB	b3421	0.88	+0.9
3,555,959	-	rtcB	b3421	0.88	+0.5
3,555,969	-	rtcB	b3421	0.89	-1.0
3,555,970	-	rtcB	b3421	0.89	+0.7
3,556,030	+				+0.5
3,556,031	+				+0.9
3,556,035	+				-0.3
3,556,035	+				-1.4
3,556,037	+				+2.4
3,556,043	-				-0.4
3,556,043	-				+1.1
3,556,092	-				+1.0
3,556,232	-				-1.8
3,556,232	-				+0.9
3,556,273	+				-1.3
3,556,292	+				+2.2
3,556,292	+				-0.1
3,556,297	+				+0.3
3,556,316	+				+0.6
3,556,326	+				+1.2
3,556,326	+				+5.1
3,556,326	+				-2.6
3,556,326	+				+1.4
3,556,326	+				-0.0
3,556,326	+				-1.8
3,556,334	-				-0.3
3,556,334	-				+4.2
3,556,334	-				-1.7
3,556,334	-				+1.4
3,556,334	-				-2.3
3,556,334	-				-1.6
3,556,358	-				+1.1
3,556,358	-				-0.3
3,556,372	+				-1.6
3,556,372	+				-1.8
3,556,380	-				-0.4
3,556,385	-				-0.8
3,556,392	-				+1.3
3,556,426	-				-1.0
3,556,548	-	rtcR	b3422	0.16	+4.1
3,556,551	+	rtcR	b3422	0.16	+3.4
3,556,731	-	rtcR	b3422	0.28	+0.3
3,556,746	-	rtcR	b3422	0.29	-2.1
3,556,746	-	rtcR	b3422	0.29	+1.8
3,556,858	+	rtcR	b3422	0.36	+1.0
3,556,858	+	rtcR	b3422	0.36	+0.5
3,556,859	+	rtcR	b3422	0.36	-0.0
3,556,933	-	rtcR	b3422	0.40	+1.8
3,556,940	-	rtcR	b3422	0.41	+0.4
3,556,956	+	rtcR	b3422	0.42	-0.7
3,556,973	-	rtcR	b3422	0.43	-0.4
3,556,973	-	rtcR	b3422	0.43	-0.5
3,556,986	-	rtcR	b3422	0.44	-1.2
3,557,003	-	rtcR	b3422	0.45	+1.5
3,557,008	-	rtcR	b3422	0.45	+0.1
3,557,019	+	rtcR	b3422	0.46	+1.5
3,557,029	+	rtcR	b3422	0.46	+1.0
3,557,034	+	rtcR	b3422	0.47	+0.5
3,557,042	-	rtcR	b3422	0.47	+0.6
3,557,042	-	rtcR	b3422	0.47	+0.3
3,557,166	+	rtcR	b3422	0.55	+2.7
3,557,179	-	rtcR	b3422	0.56	-1.3
3,557,179	-	rtcR	b3422	0.56	-0.9
3,557,218	+	rtcR	b3422	0.58	+0.7
3,557,246	+	rtcR	b3422	0.60	+0.3
3,557,246	+	rtcR	b3422	0.60	-0.0
3,557,280	+	rtcR	b3422	0.62	+2.6
3,557,345	+	rtcR	b3422	0.66	-3.1
3,557,345	+	rtcR	b3422	0.66	-2.1
3,557,347	-	rtcR	b3422	0.66	-0.5
3,557,347	-	rtcR	b3422	0.66	-2.4
3,557,368	+	rtcR	b3422	0.67	+2.0
3,557,389	+	rtcR	b3422	0.69	+1.1
3,557,506	-	rtcR	b3422	0.76	+0.8
3,557,560	-	rtcR	b3422	0.79	+0.9
3,557,577	-	rtcR	b3422	0.80	+0.1
3,557,577	-	rtcR	b3422	0.80	-0.5
3,557,583	+	rtcR	b3422	0.81	+1.1
3,557,591	-	rtcR	b3422	0.81	+0.2
3,557,603	-	rtcR	b3422	0.82	-0.2
3,557,621	+	rtcR	b3422	0.83	-0.5
3,557,673	+	rtcR	b3422	0.86	-0.2
3,557,709	-	rtcR	b3422	0.89	+1.1
3,557,709	-	rtcR	b3422	0.89	+1.3
3,557,833	-				+1.8
3,557,849	+				+1.8
3,557,849	+				-1.8
3,557,881	+				+2.2
3,557,884	+				-1.3
3,557,908	-				+2.2
3,557,908	-				+0.0
3,557,948	-	glpR	b3423	0.10	-1.5
3,557,949	+	glpR	b3423	0.10	-1.2
3,557,959	+	glpR	b3423	0.12	-0.8
3,557,968	+	glpR	b3423	0.13	+0.8
3,558,019	+	glpR	b3423	0.20	+1.0
3,558,019	+	glpR	b3423	0.20	+0.5
3,558,069	+	glpR	b3423	0.26	+0.3
3,558,069	+	glpR	b3423	0.26	+0.4
3,558,101	+	glpR	b3423	0.30	+0.4
3,558,103	-	glpR	b3423	0.31	-0.8
3,558,103	-	glpR	b3423	0.31	+3.0
3,558,103	-	glpR	b3423	0.31	-0.0
3,558,104	-	glpR	b3423	0.31	-1.6
3,558,104	-	glpR	b3423	0.31	-1.6
3,558,115	+	glpR	b3423	0.32	-0.2
3,558,143	-	glpR	b3423	0.36	+1.2
3,558,156	-	glpR	b3423	0.38	-1.9
3,558,156	-	glpR	b3423	0.38	-1.0
3,558,221	-	glpR	b3423	0.46	-1.0
3,558,266	+	glpR	b3423	0.52	-0.8
3,558,301	+	glpR	b3423	0.57	+1.4
3,558,309	-	glpR	b3423	0.58	-0.1
3,558,309	-	glpR	b3423	0.58	-0.6
3,558,309	-	glpR	b3423	0.58	+0.7
3,558,309	-	glpR	b3423	0.58	-0.9
3,558,309	-	glpR	b3423	0.58	-2.3
3,558,327	+	glpR	b3423	0.60	+0.6
3,558,364	-	glpR	b3423	0.65	-1.0
3,558,364	-	glpR	b3423	0.65	+1.8
3,558,407	+	glpR	b3423	0.71	-0.2
3,558,409	+	glpR	b3423	0.71	-0.3
3,558,415	+	glpR	b3423	0.72	-0.5
3,558,523	-	glpR	b3423	0.86	+0.1
3,558,572	-				-0.1
3,558,578	+				+2.3
3,558,578	+				-0.9
3,558,588	-				+0.1
3,558,606	+				+0.4
3,558,687	+				+0.4
3,558,753	+	glpG	b3424	0.13	-0.4
3,558,766	+	glpG	b3424	0.15	+1.1
3,558,774	-	glpG	b3424	0.16	-0.5
3,558,776	+	glpG	b3424	0.16	+1.0
3,558,776	+	glpG	b3424	0.16	-0.3
3,558,791	-	glpG	b3424	0.18	+2.0
3,558,794	-	glpG	b3424	0.18	+0.2
3,558,816	-	glpG	b3424	0.21	-0.6
3,558,851	-	glpG	b3424	0.25	-0.2
3,558,855	-	glpG	b3424	0.25	-1.6
3,558,867	-	glpG	b3424	0.27	-0.1
3,558,867	-	glpG	b3424	0.27	+0.5