Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsH and rlmE are separated by 99 nucleotides rlmE and yhbY are separated by 125 nucleotides yhbY and greA are separated by 155 nucleotides
b3178: ftsH - protease, ATP-dependent zinc-metallo (NCBI), at 3,323,023 to 3,324,957
ftsH
b3179: rlmE - 23S rRNA U2552 ribose 2'-O-methyltransferase, SAM-dependent (NCBI), at 3,325,057 to 3,325,686
rlmE
b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105
yhbY
b3181: greA - transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS), at 3,326,261 to 3,326,737
greA
Position (kb)
3325
3326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3324.988 kb on - strand at 3324.988 kb on - strand at 3325.020 kb on - strand at 3325.021 kb on - strand at 3325.022 kb on - strand at 3325.025 kb on - strand at 3325.061 kb on + strand at 3325.061 kb on + strand at 3325.064 kb on - strand at 3325.064 kb on - strand at 3325.085 kb on - strand at 3325.085 kb on - strand at 3325.749 kb on - strand at 3325.849 kb on + strand, within yhbY at 3325.849 kb on + strand at 3325.883 kb on - strand, within yhbY at 3325.908 kb on - strand, within yhbY at 3325.913 kb on + strand, within yhbY at 3325.921 kb on - strand, within yhbY at 3325.928 kb on + strand, within yhbY at 3325.932 kb on + strand, within yhbY at 3325.939 kb on + strand at 3325.940 kb on - strand, within yhbY at 3325.940 kb on - strand, within yhbY at 3325.976 kb on - strand, within yhbY at 3326.010 kb on - strand, within yhbY at 3326.015 kb on - strand, within yhbY at 3326.015 kb on - strand, within yhbY at 3326.066 kb on - strand, within yhbY at 3326.187 kb on - strand at 3326.190 kb on - strand at 3326.197 kb on + strand at 3326.267 kb on - strand at 3326.278 kb on - strand at 3326.278 kb on - strand at 3326.323 kb on + strand, within greA at 3326.338 kb on - strand, within greA at 3326.500 kb on - strand, within greA at 3326.500 kb on - strand, within greA at 3326.548 kb on + strand, within greA at 3326.548 kb on + strand, within greA at 3326.552 kb on + strand, within greA at 3326.552 kb on + strand, within greA at 3326.602 kb on - strand, within greA at 3326.653 kb on + strand, within greA
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,324,988 - -1.0 3,324,988 - -0.8 3,325,020 - -2.2 3,325,021 - +0.8 3,325,022 - +1.6 3,325,025 - +1.5 3,325,061 + +0.3 3,325,061 + +0.1 3,325,064 - +0.7 3,325,064 - -1.3 3,325,085 - -1.5 3,325,085 - -1.6 3,325,749 - -1.7 3,325,849 + yhbY b3180 0.13 -0.4 3,325,849 + +2.6 3,325,883 - yhbY b3180 0.24 -2.8 3,325,908 - yhbY b3180 0.33 -2.2 3,325,913 + yhbY b3180 0.34 -1.9 3,325,921 - yhbY b3180 0.37 -1.4 3,325,928 + yhbY b3180 0.39 -2.3 3,325,932 + yhbY b3180 0.41 +1.3 3,325,939 + +1.8 3,325,940 - yhbY b3180 0.44 -1.5 3,325,940 - yhbY b3180 0.44 -1.1 3,325,976 - yhbY b3180 0.56 -3.6 3,326,010 - yhbY b3180 0.67 +0.8 3,326,015 - yhbY b3180 0.69 -2.4 3,326,015 - yhbY b3180 0.69 -2.0 3,326,066 - yhbY b3180 0.86 -0.4 3,326,187 - +0.9 3,326,190 - +2.0 3,326,197 + -2.4 3,326,267 - +0.2 3,326,278 - -0.2 3,326,278 - -1.7 3,326,323 + greA b3181 0.13 -3.0 3,326,338 - greA b3181 0.16 -1.6 3,326,500 - greA b3181 0.50 -0.5 3,326,500 - greA b3181 0.50 -1.0 3,326,548 + greA b3181 0.60 -2.2 3,326,548 + greA b3181 0.60 -3.4 3,326,552 + greA b3181 0.61 -1.8 3,326,552 + greA b3181 0.61 -2.9 3,326,602 - greA b3181 0.71 -0.9 3,326,653 + greA b3181 0.82 -2.8
Or see this region's nucleotide sequence