Strain Fitness in Escherichia coli BW25113 around b2962

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmutY and yggX are separated by 27 nucleotidesyggX and mltC are separated by 64 nucleotides b2961: mutY - adenine DNA glycosylase (NCBI), at 3,101,035 to 3,102,087 mutY b2962: yggX - hypothetical protein (NCBI), at 3,102,115 to 3,102,390 yggX b2963: mltC - membrane-bound lytic murein transglycosylase C (VIMSS), at 3,102,455 to 3,103,534 mltC Position (kb) 3102 3103Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3101.122 kb on + strandat 3101.122 kb on + strandat 3101.124 kb on + strandat 3101.124 kb on + strandat 3101.251 kb on + strand, within mutYat 3101.251 kb on + strand, within mutYat 3101.259 kb on + strand, within mutYat 3101.259 kb on + strand, within mutYat 3101.430 kb on + strand, within mutYat 3101.430 kb on + strand, within mutYat 3101.456 kb on - strand, within mutYat 3101.462 kb on - strand, within mutYat 3101.462 kb on - strand, within mutYat 3101.483 kb on - strand, within mutYat 3101.483 kb on - strand, within mutYat 3101.483 kb on - strand, within mutYat 3101.483 kb on - strand, within mutYat 3101.561 kb on + strand, within mutYat 3101.605 kb on + strand, within mutYat 3101.620 kb on + strand, within mutYat 3101.811 kb on - strand, within mutYat 3101.911 kb on + strand, within mutYat 3101.942 kb on + strand, within mutYat 3102.040 kb on + strandat 3102.152 kb on + strand, within yggXat 3102.350 kb on - strand, within yggXat 3102.350 kb on - strand, within yggXat 3102.352 kb on + strand, within yggXat 3102.352 kb on + strand, within yggXat 3102.352 kb on - strand, within yggXat 3102.443 kb on - strandat 3102.604 kb on + strand, within mltCat 3102.604 kb on + strand, within mltCat 3102.612 kb on - strand, within mltCat 3102.622 kb on - strand, within mltCat 3102.623 kb on - strand, within mltCat 3102.623 kb on - strand, within mltCat 3102.631 kb on - strand, within mltCat 3102.636 kb on + strand, within mltCat 3102.636 kb on + strand, within mltCat 3102.636 kb on + strand, within mltCat 3102.638 kb on + strand, within mltCat 3102.638 kb on + strand, within mltCat 3102.644 kb on - strand, within mltCat 3102.644 kb on - strand, within mltCat 3102.646 kb on - strand, within mltCat 3102.648 kb on + strand, within mltCat 3102.648 kb on + strand, within mltCat 3102.676 kb on - strand, within mltCat 3102.694 kb on + strand, within mltCat 3102.700 kb on - strand, within mltCat 3102.700 kb on - strand, within mltCat 3102.729 kb on + strand, within mltCat 3102.729 kb on + strand, within mltCat 3102.737 kb on - strand, within mltCat 3102.737 kb on - strand, within mltCat 3102.789 kb on + strand, within mltCat 3102.816 kb on - strand, within mltCat 3102.858 kb on + strand, within mltCat 3102.868 kb on + strand, within mltCat 3102.960 kb on - strand, within mltCat 3102.964 kb on + strand, within mltCat 3102.964 kb on + strand, within mltCat 3102.973 kb on + strand, within mltCat 3102.974 kb on - strand, within mltCat 3102.974 kb on - strand, within mltCat 3102.985 kb on + strand, within mltCat 3102.985 kb on + strand, within mltCat 3102.992 kb on - strand, within mltCat 3102.995 kb on - strand, within mltCat 3103.017 kb on - strand, within mltCat 3103.024 kb on + strand, within mltCat 3103.118 kb on - strand, within mltCat 3103.165 kb on + strand, within mltCat 3103.175 kb on + strand, within mltCat 3103.176 kb on - strand, within mltCat 3103.187 kb on - strand, within mltCat 3103.187 kb on - strand, within mltCat 3103.283 kb on - strand, within mltCat 3103.314 kb on + strand, within mltC

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,101,122 + +2.0
3,101,122 + +0.4
3,101,124 + -2.3
3,101,124 + +2.2
3,101,251 + mutY b2961 0.21 +1.1
3,101,251 + mutY b2961 0.21 +1.4
3,101,259 + mutY b2961 0.21 -1.4
3,101,259 + mutY b2961 0.21 -2.9
3,101,430 + mutY b2961 0.38 +0.4
3,101,430 + mutY b2961 0.38 +1.0
3,101,456 - mutY b2961 0.40 +1.6
3,101,462 - mutY b2961 0.41 -0.8
3,101,462 - mutY b2961 0.41 +1.9
3,101,483 - mutY b2961 0.43 +0.4
3,101,483 - mutY b2961 0.43 +1.5
3,101,483 - mutY b2961 0.43 -2.4
3,101,483 - mutY b2961 0.43 +0.3
3,101,561 + mutY b2961 0.50 -0.1
3,101,605 + mutY b2961 0.54 +1.5
3,101,620 + mutY b2961 0.56 +1.1
3,101,811 - mutY b2961 0.74 -0.4
3,101,911 + mutY b2961 0.83 -1.0
3,101,942 + mutY b2961 0.86 -0.6
3,102,040 + +0.6
3,102,152 + yggX b2962 0.13 -0.1
3,102,350 - yggX b2962 0.85 -2.1
3,102,350 - yggX b2962 0.85 +0.1
3,102,352 + yggX b2962 0.86 -1.1
3,102,352 + yggX b2962 0.86 -1.4
3,102,352 - yggX b2962 0.86 +2.5
3,102,443 - +2.4
3,102,604 + mltC b2963 0.14 -1.4
3,102,604 + mltC b2963 0.14 -0.5
3,102,612 - mltC b2963 0.15 +1.3
3,102,622 - mltC b2963 0.15 -0.3
3,102,623 - mltC b2963 0.16 -0.1
3,102,623 - mltC b2963 0.16 -0.2
3,102,631 - mltC b2963 0.16 -1.3
3,102,636 + mltC b2963 0.17 +1.2
3,102,636 + mltC b2963 0.17 -1.0
3,102,636 + mltC b2963 0.17 -0.2
3,102,638 + mltC b2963 0.17 -1.4
3,102,638 + mltC b2963 0.17 -1.3
3,102,644 - mltC b2963 0.17 -1.7
3,102,644 - mltC b2963 0.17 +1.6
3,102,646 - mltC b2963 0.18 +2.6
3,102,648 + mltC b2963 0.18 -0.5
3,102,648 + mltC b2963 0.18 +0.7
3,102,676 - mltC b2963 0.20 -0.6
3,102,694 + mltC b2963 0.22 -1.2
3,102,700 - mltC b2963 0.23 -1.3
3,102,700 - mltC b2963 0.23 +2.6
3,102,729 + mltC b2963 0.25 +2.5
3,102,729 + mltC b2963 0.25 -0.2
3,102,737 - mltC b2963 0.26 +0.6
3,102,737 - mltC b2963 0.26 -0.9
3,102,789 + mltC b2963 0.31 +0.9
3,102,816 - mltC b2963 0.33 -1.3
3,102,858 + mltC b2963 0.37 -0.1
3,102,868 + mltC b2963 0.38 -2.0
3,102,960 - mltC b2963 0.47 +1.4
3,102,964 + mltC b2963 0.47 +0.5
3,102,964 + mltC b2963 0.47 -0.3
3,102,973 + mltC b2963 0.48 +1.3
3,102,974 - mltC b2963 0.48 -2.2
3,102,974 - mltC b2963 0.48 -1.7
3,102,985 + mltC b2963 0.49 +1.9
3,102,985 + mltC b2963 0.49 -1.4
3,102,992 - mltC b2963 0.50 +1.4
3,102,995 - mltC b2963 0.50 -0.4
3,103,017 - mltC b2963 0.52 +0.7
3,103,024 + mltC b2963 0.53 +0.1
3,103,118 - mltC b2963 0.61 +0.8
3,103,165 + mltC b2963 0.66 -1.8
3,103,175 + mltC b2963 0.67 -1.4
3,103,176 - mltC b2963 0.67 -0.0
3,103,187 - mltC b2963 0.68 -0.8
3,103,187 - mltC b2963 0.68 -0.5
3,103,283 - mltC b2963 0.77 +2.2
3,103,314 + mltC b2963 0.80 +0.2

Or see this region's nucleotide sequence