Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yliK and argK overlap by 8 nucleotides argK and ygfG are separated by 10 nucleotides ygfG and ygfH are separated by 23 nucleotides
b2917: yliK - methylmalonyl-CoA mutase (NCBI), at 3,058,872 to 3,061,016
yliK
b2918: argK - arginine/ornithine transport system ATPase (NCBI), at 3,061,009 to 3,062,004
argK
b2919: ygfG - putative enzyme (VIMSS), at 3,062,015 to 3,062,800
ygfG
b2920: ygfH - propionyl-CoA:succinate-CoA transferase (NCBI), at 3,062,824 to 3,064,302
ygfH
Position (kb)
3061
3062
3063 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3060.026 kb on + strand, within yliK at 3060.064 kb on - strand, within yliK at 3060.068 kb on + strand, within yliK at 3060.151 kb on + strand, within yliK at 3060.215 kb on + strand, within yliK at 3060.350 kb on - strand, within yliK at 3060.369 kb on + strand, within yliK at 3060.445 kb on + strand, within yliK at 3060.458 kb on - strand, within yliK at 3060.479 kb on - strand, within yliK at 3060.515 kb on - strand, within yliK at 3060.592 kb on - strand, within yliK at 3060.592 kb on - strand, within yliK at 3060.662 kb on - strand, within yliK at 3060.741 kb on + strand, within yliK at 3060.741 kb on + strand, within yliK at 3060.826 kb on + strand at 3060.826 kb on + strand at 3060.926 kb on + strand at 3060.958 kb on + strand at 3060.958 kb on + strand at 3060.963 kb on + strand at 3061.019 kb on + strand at 3061.019 kb on + strand at 3061.082 kb on - strand at 3061.088 kb on - strand at 3061.127 kb on + strand, within argK at 3061.127 kb on + strand, within argK at 3061.292 kb on - strand, within argK at 3061.292 kb on - strand, within argK at 3061.372 kb on + strand, within argK at 3061.387 kb on + strand, within argK at 3061.397 kb on + strand, within argK at 3061.561 kb on - strand, within argK at 3061.654 kb on + strand, within argK at 3061.654 kb on + strand, within argK at 3062.118 kb on + strand, within ygfG at 3062.216 kb on + strand, within ygfG at 3062.251 kb on + strand, within ygfG at 3062.256 kb on + strand, within ygfG at 3062.256 kb on + strand, within ygfG at 3062.328 kb on + strand, within ygfG at 3062.328 kb on + strand, within ygfG at 3062.364 kb on + strand, within ygfG at 3062.390 kb on + strand, within ygfG at 3062.411 kb on + strand, within ygfG at 3062.438 kb on - strand, within ygfG at 3062.438 kb on - strand, within ygfG at 3062.608 kb on - strand, within ygfG at 3062.608 kb on - strand, within ygfG at 3062.651 kb on + strand, within ygfG at 3062.723 kb on + strand at 3062.731 kb on - strand at 3062.731 kb on - strand at 3062.785 kb on - strand at 3062.929 kb on + strand at 3062.937 kb on - strand at 3062.937 kb on - strand at 3062.937 kb on - strand at 3062.963 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,060,026 + yliK b2917 0.54 +1.7 3,060,064 - yliK b2917 0.56 +0.9 3,060,068 + yliK b2917 0.56 -1.6 3,060,151 + yliK b2917 0.60 -1.4 3,060,215 + yliK b2917 0.63 +0.1 3,060,350 - yliK b2917 0.69 +0.6 3,060,369 + yliK b2917 0.70 +1.5 3,060,445 + yliK b2917 0.73 -1.5 3,060,458 - yliK b2917 0.74 +1.7 3,060,479 - yliK b2917 0.75 +0.0 3,060,515 - yliK b2917 0.77 +0.4 3,060,592 - yliK b2917 0.80 -0.8 3,060,592 - yliK b2917 0.80 +0.8 3,060,662 - yliK b2917 0.83 -1.3 3,060,741 + yliK b2917 0.87 +1.8 3,060,741 + yliK b2917 0.87 -0.4 3,060,826 + +1.2 3,060,826 + +1.7 3,060,926 + -2.1 3,060,958 + -0.1 3,060,958 + -2.2 3,060,963 + +0.5 3,061,019 + -1.5 3,061,019 + -0.9 3,061,082 - +0.5 3,061,088 - -0.3 3,061,127 + argK b2918 0.12 -1.8 3,061,127 + argK b2918 0.12 -1.9 3,061,292 - argK b2918 0.28 -2.2 3,061,292 - argK b2918 0.28 -2.3 3,061,372 + argK b2918 0.36 -2.4 3,061,387 + argK b2918 0.38 +1.2 3,061,397 + argK b2918 0.39 -0.3 3,061,561 - argK b2918 0.55 -0.8 3,061,654 + argK b2918 0.65 +0.5 3,061,654 + argK b2918 0.65 -2.5 3,062,118 + ygfG b2919 0.13 +0.8 3,062,216 + ygfG b2919 0.26 -0.3 3,062,251 + ygfG b2919 0.30 +0.8 3,062,256 + ygfG b2919 0.31 -0.8 3,062,256 + ygfG b2919 0.31 +2.0 3,062,328 + ygfG b2919 0.40 -1.9 3,062,328 + ygfG b2919 0.40 -1.1 3,062,364 + ygfG b2919 0.44 -3.9 3,062,390 + ygfG b2919 0.48 -1.8 3,062,411 + ygfG b2919 0.50 -2.0 3,062,438 - ygfG b2919 0.54 +1.6 3,062,438 - ygfG b2919 0.54 -0.7 3,062,608 - ygfG b2919 0.75 +1.8 3,062,608 - ygfG b2919 0.75 +0.7 3,062,651 + ygfG b2919 0.81 -2.6 3,062,723 + +1.8 3,062,731 - -1.7 3,062,731 - +1.6 3,062,785 - -1.4 3,062,929 + +0.4 3,062,937 - +1.8 3,062,937 - +0.6 3,062,937 - +0.4 3,062,963 - +1.5
Or see this region's nucleotide sequence