Strain Fitness in Escherichia coli BW25113 around b2705

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
2,824,775	+	srlE	b2703	0.38	-0.7
2,824,933	-	srlE	b2703	0.54	-0.3
2,824,938	-	srlE	b2703	0.55	+1.3
2,824,938	-	srlE	b2703	0.55	-0.9
2,824,969	+	srlE	b2703	0.58	+1.5
2,824,969	+	srlE	b2703	0.58	-1.8
2,824,983	+	srlE	b2703	0.59	+0.9
2,824,983	+	srlE	b2703	0.59	+2.2
2,825,009	+	srlE	b2703	0.62	-0.2
2,825,113	-	srlE	b2703	0.73	+0.1
2,825,179	+	srlE	b2703	0.80	-2.4
2,825,263	+	srlE	b2703	0.88	-1.1
2,825,263	+	srlE	b2703	0.88	+1.6
2,825,296	+				+0.1
2,825,569	+	srlB	b2704	0.50	+0.1
2,825,599	-	srlB	b2704	0.58	+0.3
2,825,631	+	srlB	b2704	0.66	+1.7
2,825,631	+	srlB	b2704	0.66	+0.4
2,825,641	+	srlB	b2704	0.69	-0.9
2,825,689	-	srlB	b2704	0.82	-0.8
2,825,709	+	srlB	b2704	0.87	-1.3
2,825,734	+				-1.9
2,825,807	+				+0.6
2,825,921	-	srlD	b2705	0.21	-0.0
2,825,949	-	srlD	b2705	0.24	+0.5
2,825,949	-	srlD	b2705	0.24	+1.3
2,825,973	+	srlD	b2705	0.27	-0.7
2,826,044	-	srlD	b2705	0.37	-0.9
2,826,210	+	srlD	b2705	0.58	+1.4
2,826,210	+	srlD	b2705	0.58	+1.2
2,826,215	+	srlD	b2705	0.58	+0.5
2,826,218	-	srlD	b2705	0.59	+1.5
2,826,218	-	srlD	b2705	0.59	-2.1
2,826,245	+	srlD	b2705	0.62	-2.2
2,826,318	-	srlD	b2705	0.72	-1.2
2,826,397	+	srlD	b2705	0.82	-1.0
2,826,397	+	srlD	b2705	0.82	+1.5
2,826,397	+	srlD	b2705	0.82	-0.9
2,826,397	+	srlD	b2705	0.82	-1.9
2,826,474	+				-1.0
2,826,474	+				-0.4
2,826,482	-				+0.0
2,826,490	-				+1.8
2,826,490	-				+0.0
2,826,613	-				+1.7
2,826,613	-				-1.0
2,826,613	-				+1.1
2,826,646	-				+0.9
2,826,771	-	gutM	b2706	0.36	+1.8
2,826,876	-	gutM	b2706	0.65	+2.7
2,826,932	+	gutM	b2706	0.80	-1.0
2,826,963	-	gutM	b2706	0.89	+0.2
2,827,166	-	srlR	b2707	0.13	-2.2
2,827,166	-	srlR	b2707	0.13	-1.7
2,827,191	-	srlR	b2707	0.16	-2.9
2,827,191	-	srlR	b2707	0.16	-1.7
2,827,193	+	srlR	b2707	0.16	-2.5
2,827,193	+	srlR	b2707	0.16	-4.7
2,827,201	-	srlR	b2707	0.17	-1.4
2,827,298	+	srlR	b2707	0.30	-2.3
2,827,368	+	srlR	b2707	0.39	-1.1
2,827,425	+	srlR	b2707	0.46	-0.1
2,827,431	+	srlR	b2707	0.47	+2.7
2,827,481	+	srlR	b2707	0.53	-0.3
2,827,483	-	srlR	b2707	0.53	-1.2