Strain Fitness in Escherichia coli BW25113 around b2129

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlrA and yohO are separated by 59 nucleotidesyohO and yehW overlap by 20 nucleotidesyehW and yehX are separated by 4 nucleotidesyehX and yehY overlap by 8 nucleotides b2127: mlrA - DNA-binding transcriptional regulator (NCBI), at 2,212,888 to 2,213,619 mlrA b4542: yohO - hypothetical protein (NCBI), at 2,213,679 to 2,213,786 yohO b2128: yehW - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,213,767 to 2,214,498 yehW b2129: yehX - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 2,214,503 to 2,215,429 yehX b2130: yehY - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,215,422 to 2,216,579 yehY Position (kb) 2214 2215 2216Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2213.506 kb on + strand, within mlrAat 2213.506 kb on + strand, within mlrAat 2213.513 kb on + strand, within mlrAat 2213.641 kb on + strandat 2213.641 kb on + strandat 2213.646 kb on + strandat 2213.654 kb on - strandat 2213.684 kb on - strandat 2213.781 kb on + strandat 2213.819 kb on + strandat 2213.903 kb on - strand, within yehWat 2213.988 kb on - strand, within yehWat 2214.022 kb on - strand, within yehWat 2214.159 kb on + strand, within yehWat 2214.356 kb on - strand, within yehWat 2214.356 kb on - strand, within yehWat 2214.491 kb on + strandat 2214.491 kb on + strandat 2214.540 kb on + strandat 2214.540 kb on + strandat 2214.554 kb on - strandat 2214.568 kb on - strandat 2214.700 kb on + strand, within yehXat 2214.708 kb on - strand, within yehXat 2214.965 kb on + strand, within yehXat 2214.997 kb on + strand, within yehXat 2215.044 kb on - strand, within yehXat 2215.044 kb on - strand, within yehXat 2215.187 kb on - strand, within yehXat 2215.394 kb on + strandat 2215.450 kb on + strandat 2215.461 kb on + strandat 2215.683 kb on - strand, within yehYat 2215.683 kb on - strand, within yehYat 2215.698 kb on - strand, within yehYat 2215.698 kb on - strand, within yehYat 2215.701 kb on - strand, within yehYat 2215.805 kb on + strand, within yehYat 2215.876 kb on - strand, within yehYat 2215.909 kb on - strand, within yehYat 2215.909 kb on - strand, within yehYat 2215.998 kb on + strand, within yehYat 2216.025 kb on + strand, within yehYat 2216.025 kb on + strand, within yehYat 2216.209 kb on + strand, within yehYat 2216.209 kb on + strand, within yehYat 2216.217 kb on - strand, within yehYat 2216.217 kb on - strand, within yehYat 2216.355 kb on - strand, within yehY

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,213,506 + mlrA b2127 0.84 -1.9
2,213,506 + mlrA b2127 0.84 +2.2
2,213,513 + mlrA b2127 0.85 +0.1
2,213,641 + +1.6
2,213,641 + -1.8
2,213,646 + -1.5
2,213,654 - -1.6
2,213,684 - +1.2
2,213,781 + +1.6
2,213,819 + -0.5
2,213,903 - yehW b2128 0.19 -2.2
2,213,988 - yehW b2128 0.30 -0.6
2,214,022 - yehW b2128 0.35 +0.3
2,214,159 + yehW b2128 0.54 -1.0
2,214,356 - yehW b2128 0.80 -0.8
2,214,356 - yehW b2128 0.80 +0.3
2,214,491 + -1.6
2,214,491 + +0.8
2,214,540 + +0.6
2,214,540 + +0.5
2,214,554 - +1.0
2,214,568 - -1.9
2,214,700 + yehX b2129 0.21 -0.2
2,214,708 - yehX b2129 0.22 -0.8
2,214,965 + yehX b2129 0.50 -1.9
2,214,997 + yehX b2129 0.53 -0.9
2,215,044 - yehX b2129 0.58 -1.0
2,215,044 - yehX b2129 0.58 +3.8
2,215,187 - yehX b2129 0.74 -0.9
2,215,394 + -0.3
2,215,450 + +0.5
2,215,461 + +0.5
2,215,683 - yehY b2130 0.23 +0.1
2,215,683 - yehY b2130 0.23 -1.1
2,215,698 - yehY b2130 0.24 +1.1
2,215,698 - yehY b2130 0.24 -0.6
2,215,701 - yehY b2130 0.24 -0.5
2,215,805 + yehY b2130 0.33 -0.2
2,215,876 - yehY b2130 0.39 +1.8
2,215,909 - yehY b2130 0.42 -1.5
2,215,909 - yehY b2130 0.42 -1.2
2,215,998 + yehY b2130 0.50 +2.1
2,216,025 + yehY b2130 0.52 +0.8
2,216,025 + yehY b2130 0.52 +0.5
2,216,209 + yehY b2130 0.68 +0.6
2,216,209 + yehY b2130 0.68 -2.0
2,216,217 - yehY b2130 0.69 -0.4
2,216,217 - yehY b2130 0.69 -2.2
2,216,355 - yehY b2130 0.81 +2.4

Or see this region's nucleotide sequence