Strain Fitness in Escherichia coli BW25113 around b1968

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthchA and yedV are separated by 107 nucleotidesyedV and yedW overlap by 1 nucleotidesyedW and yedX are separated by 132 nucleotides b1967: hchA - chaperone protein HchA (NCBI), at 2,033,859 to 2,034,710 hchA b1968: yedV - predicted sensory kinase in two-component regulatory system with YedW (NCBI), at 2,034,818 to 2,036,176 yedV b1969: yedW - putative 2-component transcriptional regulator (VIMSS), at 2,036,176 to 2,036,847 yedW b1970: yedX - hypothetical protein (NCBI), at 2,036,980 to 2,037,393 yedX Position (kb) 2034 2035 2036 2037Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2033.855 kb on + strandat 2033.863 kb on - strandat 2033.863 kb on - strandat 2033.865 kb on + strandat 2033.897 kb on - strandat 2033.931 kb on - strandat 2033.953 kb on - strand, within hchAat 2033.953 kb on - strand, within hchAat 2033.957 kb on - strand, within hchAat 2033.970 kb on + strand, within hchAat 2033.975 kb on - strand, within hchAat 2033.986 kb on - strand, within hchAat 2033.988 kb on - strand, within hchAat 2033.988 kb on - strand, within hchAat 2033.988 kb on - strand, within hchAat 2034.035 kb on + strand, within hchAat 2034.070 kb on + strand, within hchAat 2034.070 kb on - strand, within hchAat 2034.077 kb on - strand, within hchAat 2034.093 kb on + strand, within hchAat 2034.093 kb on + strand, within hchAat 2034.112 kb on - strand, within hchAat 2034.118 kb on - strand, within hchAat 2034.156 kb on - strand, within hchAat 2034.168 kb on + strand, within hchAat 2034.224 kb on + strand, within hchAat 2034.224 kb on + strand, within hchAat 2034.356 kb on - strand, within hchAat 2034.364 kb on - strand, within hchAat 2034.371 kb on + strand, within hchAat 2034.371 kb on + strand, within hchAat 2034.374 kb on - strand, within hchAat 2034.374 kb on - strand, within hchAat 2034.444 kb on - strand, within hchAat 2034.491 kb on - strand, within hchAat 2034.514 kb on - strand, within hchAat 2034.514 kb on - strand, within hchAat 2034.522 kb on - strand, within hchAat 2034.620 kb on - strand, within hchAat 2034.620 kb on - strand, within hchAat 2034.637 kb on + strandat 2034.637 kb on + strandat 2034.683 kb on + strandat 2034.713 kb on - strandat 2034.741 kb on - strandat 2034.754 kb on + strandat 2034.849 kb on + strandat 2034.872 kb on - strandat 2034.874 kb on + strandat 2034.875 kb on - strandat 2034.884 kb on + strandat 2034.885 kb on + strandat 2034.903 kb on - strandat 2034.928 kb on + strandat 2034.928 kb on + strandat 2034.928 kb on + strandat 2034.936 kb on - strandat 2035.040 kb on - strand, within yedVat 2035.222 kb on - strand, within yedVat 2035.563 kb on - strand, within yedVat 2035.853 kb on - strand, within yedVat 2035.861 kb on - strand, within yedVat 2035.894 kb on - strand, within yedVat 2035.909 kb on - strand, within yedVat 2035.916 kb on - strand, within yedVat 2035.916 kb on - strand, within yedVat 2035.933 kb on - strand, within yedVat 2035.942 kb on - strand, within yedVat 2035.979 kb on - strand, within yedVat 2035.979 kb on - strand, within yedVat 2035.981 kb on - strand, within yedVat 2035.983 kb on - strand, within yedVat 2035.983 kb on - strand, within yedVat 2035.986 kb on - strand, within yedVat 2035.995 kb on - strand, within yedVat 2036.012 kb on - strand, within yedVat 2036.015 kb on - strand, within yedVat 2036.015 kb on - strand, within yedVat 2036.015 kb on - strand, within yedVat 2036.029 kb on - strand, within yedVat 2036.034 kb on - strand, within yedVat 2036.042 kb on - strandat 2036.052 kb on - strandat 2036.121 kb on - strandat 2036.165 kb on - strandat 2036.203 kb on + strandat 2036.217 kb on - strandat 2036.272 kb on - strand, within yedWat 2036.296 kb on - strand, within yedWat 2036.296 kb on - strand, within yedWat 2036.368 kb on - strand, within yedWat 2036.374 kb on + strand, within yedWat 2036.382 kb on - strand, within yedWat 2036.439 kb on + strand, within yedWat 2036.439 kb on + strand, within yedWat 2036.439 kb on + strand, within yedWat 2036.581 kb on - strand, within yedWat 2036.582 kb on + strand, within yedWat 2036.582 kb on + strand, within yedWat 2036.582 kb on + strand, within yedWat 2036.583 kb on - strand, within yedWat 2036.583 kb on - strand, within yedWat 2036.589 kb on - strand, within yedWat 2036.591 kb on - strand, within yedWat 2036.591 kb on - strand, within yedWat 2036.591 kb on - strand, within yedWat 2036.593 kb on + strand, within yedWat 2036.606 kb on - strand, within yedWat 2036.608 kb on - strand, within yedWat 2036.608 kb on - strand, within yedWat 2036.683 kb on - strand, within yedWat 2036.688 kb on + strand, within yedWat 2036.719 kb on - strand, within yedWat 2036.719 kb on - strand, within yedWat 2036.721 kb on - strand, within yedWat 2036.732 kb on + strand, within yedWat 2036.734 kb on + strand, within yedWat 2036.757 kb on + strand, within yedWat 2036.757 kb on + strand, within yedWat 2036.766 kb on + strand, within yedWat 2036.780 kb on + strandat 2036.788 kb on - strandat 2036.790 kb on + strandat 2036.790 kb on + strandat 2036.790 kb on + strandat 2036.791 kb on + strandat 2036.798 kb on - strandat 2036.798 kb on - strandat 2036.801 kb on + strandat 2036.805 kb on + strandat 2036.871 kb on + strandat 2036.913 kb on + strandat 2036.913 kb on + strandat 2036.922 kb on - strandat 2036.927 kb on - strandat 2036.927 kb on - strandat 2036.927 kb on - strandat 2036.934 kb on + strandat 2036.934 kb on + strandat 2036.946 kb on + strandat 2036.970 kb on + strandat 2036.995 kb on + strandat 2036.995 kb on + strandat 2037.001 kb on - strandat 2037.003 kb on - strandat 2037.003 kb on - strandat 2037.009 kb on + strandat 2037.013 kb on - strandat 2037.013 kb on - strandat 2037.052 kb on - strand, within yedXat 2037.060 kb on + strand, within yedXat 2037.102 kb on - strand, within yedXat 2037.102 kb on - strand, within yedXat 2037.112 kb on - strand, within yedXat 2037.118 kb on + strand, within yedXat 2037.150 kb on + strand, within yedXat 2037.150 kb on + strand, within yedXat 2037.150 kb on + strand, within yedX

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,033,855 + -1.3
2,033,863 - -1.5
2,033,863 - -2.2
2,033,865 + -1.8
2,033,897 - +0.9
2,033,931 - -1.8
2,033,953 - hchA b1967 0.11 +0.3
2,033,953 - hchA b1967 0.11 -0.1
2,033,957 - hchA b1967 0.12 -0.3
2,033,970 + hchA b1967 0.13 -0.4
2,033,975 - hchA b1967 0.14 +0.5
2,033,986 - hchA b1967 0.15 +0.9
2,033,988 - hchA b1967 0.15 -0.5
2,033,988 - hchA b1967 0.15 -0.0
2,033,988 - hchA b1967 0.15 +1.3
2,034,035 + hchA b1967 0.21 -2.0
2,034,070 + hchA b1967 0.25 +0.8
2,034,070 - hchA b1967 0.25 +0.9
2,034,077 - hchA b1967 0.26 -1.8
2,034,093 + hchA b1967 0.27 -1.9
2,034,093 + hchA b1967 0.27 +2.2
2,034,112 - hchA b1967 0.30 +1.3
2,034,118 - hchA b1967 0.30 -2.1
2,034,156 - hchA b1967 0.35 -2.1
2,034,168 + hchA b1967 0.36 +0.7
2,034,224 + hchA b1967 0.43 -1.5
2,034,224 + hchA b1967 0.43 +2.3
2,034,356 - hchA b1967 0.58 -0.1
2,034,364 - hchA b1967 0.59 -0.6
2,034,371 + hchA b1967 0.60 -0.0
2,034,371 + hchA b1967 0.60 +0.1
2,034,374 - hchA b1967 0.60 +1.3
2,034,374 - hchA b1967 0.60 +1.0
2,034,444 - hchA b1967 0.69 +1.2
2,034,491 - hchA b1967 0.74 -2.4
2,034,514 - hchA b1967 0.77 -1.2
2,034,514 - hchA b1967 0.77 -0.8
2,034,522 - hchA b1967 0.78 +1.8
2,034,620 - hchA b1967 0.89 -1.5
2,034,620 - hchA b1967 0.89 +0.4
2,034,637 + -0.2
2,034,637 + -0.9
2,034,683 + +0.3
2,034,713 - +0.5
2,034,741 - +0.6
2,034,754 + -2.0
2,034,849 + +0.5
2,034,872 - -2.3
2,034,874 + -0.5
2,034,875 - -1.9
2,034,884 + +0.0
2,034,885 + -2.0
2,034,903 - -2.2
2,034,928 + -1.7
2,034,928 + -1.3
2,034,928 + -3.2
2,034,936 - -1.5
2,035,040 - yedV b1968 0.16 -0.1
2,035,222 - yedV b1968 0.30 -2.1
2,035,563 - yedV b1968 0.55 -2.6
2,035,853 - yedV b1968 0.76 -0.8
2,035,861 - yedV b1968 0.77 +0.4
2,035,894 - yedV b1968 0.79 -1.4
2,035,909 - yedV b1968 0.80 -1.3
2,035,916 - yedV b1968 0.81 -1.5
2,035,916 - yedV b1968 0.81 -2.9
2,035,933 - yedV b1968 0.82 -0.2
2,035,942 - yedV b1968 0.83 +2.9
2,035,979 - yedV b1968 0.85 -1.8
2,035,979 - yedV b1968 0.85 +0.5
2,035,981 - yedV b1968 0.86 +2.0
2,035,983 - yedV b1968 0.86 +0.2
2,035,983 - yedV b1968 0.86 -1.1
2,035,986 - yedV b1968 0.86 +1.4
2,035,995 - yedV b1968 0.87 +0.1
2,036,012 - yedV b1968 0.88 -1.8
2,036,015 - yedV b1968 0.88 +0.6
2,036,015 - yedV b1968 0.88 +1.8
2,036,015 - yedV b1968 0.88 +0.1
2,036,029 - yedV b1968 0.89 +1.1
2,036,034 - yedV b1968 0.89 -0.9
2,036,042 - -0.5
2,036,052 - -1.5
2,036,121 - +1.0
2,036,165 - +2.2
2,036,203 + -0.7
2,036,217 - +1.6
2,036,272 - yedW b1969 0.14 +0.6
2,036,296 - yedW b1969 0.18 -0.2
2,036,296 - yedW b1969 0.18 -1.5
2,036,368 - yedW b1969 0.29 -0.5
2,036,374 + yedW b1969 0.29 -2.3
2,036,382 - yedW b1969 0.31 -1.7
2,036,439 + yedW b1969 0.39 -2.9
2,036,439 + yedW b1969 0.39 -1.5
2,036,439 + yedW b1969 0.39 -0.2
2,036,581 - yedW b1969 0.60 +2.2
2,036,582 + yedW b1969 0.60 -1.2
2,036,582 + yedW b1969 0.60 -2.1
2,036,582 + yedW b1969 0.60 -1.2
2,036,583 - yedW b1969 0.61 -1.0
2,036,583 - yedW b1969 0.61 -0.7
2,036,589 - yedW b1969 0.61 -2.4
2,036,591 - yedW b1969 0.62 -4.0
2,036,591 - yedW b1969 0.62 -0.8
2,036,591 - yedW b1969 0.62 -0.9
2,036,593 + yedW b1969 0.62 -0.9
2,036,606 - yedW b1969 0.64 +0.3
2,036,608 - yedW b1969 0.64 -1.3
2,036,608 - yedW b1969 0.64 -0.3
2,036,683 - yedW b1969 0.75 +0.3
2,036,688 + yedW b1969 0.76 -1.4
2,036,719 - yedW b1969 0.81 -0.5
2,036,719 - yedW b1969 0.81 +1.1
2,036,721 - yedW b1969 0.81 -2.1
2,036,732 + yedW b1969 0.83 -0.2
2,036,734 + yedW b1969 0.83 +0.8
2,036,757 + yedW b1969 0.86 +0.6
2,036,757 + yedW b1969 0.86 +3.0
2,036,766 + yedW b1969 0.88 +1.6
2,036,780 + -0.1
2,036,788 - -3.1
2,036,790 + +1.0
2,036,790 + +1.1
2,036,790 + +1.2
2,036,791 + -1.2
2,036,798 - -1.9
2,036,798 - -3.4
2,036,801 + +0.6
2,036,805 + +1.1
2,036,871 + -1.9
2,036,913 + -0.5
2,036,913 + -2.3
2,036,922 - -2.1
2,036,927 - -2.1
2,036,927 - +1.0
2,036,927 - -1.5
2,036,934 + +3.5
2,036,934 + +1.5
2,036,946 + +0.4
2,036,970 + +0.2
2,036,995 + -2.1
2,036,995 + -1.5
2,037,001 - -1.5
2,037,003 - -0.5
2,037,003 - +2.1
2,037,009 + -1.8
2,037,013 - +0.4
2,037,013 - -0.8
2,037,052 - yedX b1970 0.17 +0.3
2,037,060 + yedX b1970 0.19 -0.5
2,037,102 - yedX b1970 0.29 -0.6
2,037,102 - yedX b1970 0.29 -2.1
2,037,112 - yedX b1970 0.32 -1.1
2,037,118 + yedX b1970 0.33 +0.6
2,037,150 + yedX b1970 0.41 +1.3
2,037,150 + yedX b1970 0.41 +0.8
2,037,150 + yedX b1970 0.41 -0.6

Or see this region's nucleotide sequence