Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheA and motB are separated by 4 nucleotides motB and motA overlap by 4 nucleotides motA and flhC are separated by 126 nucleotides flhC and flhD are separated by 2 nucleotides
b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348
cheA
b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279
motB
b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163
motA
b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868
flhC
b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221
flhD
Position (kb)
1974
1975
1976 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1973.279 kb on - strand at 1973.279 kb on - strand at 1973.443 kb on - strand at 1973.443 kb on - strand at 1973.577 kb on - strand, within motB at 1973.595 kb on - strand, within motB at 1973.697 kb on + strand, within motB at 1973.846 kb on - strand, within motB at 1973.850 kb on - strand, within motB at 1974.089 kb on - strand, within motB at 1974.089 kb on - strand, within motB at 1974.197 kb on + strand at 1974.226 kb on + strand at 1974.233 kb on + strand at 1974.233 kb on + strand at 1974.414 kb on + strand, within motA at 1974.854 kb on - strand, within motA at 1974.934 kb on + strand, within motA at 1974.934 kb on + strand, within motA at 1975.040 kb on + strand, within motA at 1975.092 kb on + strand at 1975.093 kb on - strand at 1975.173 kb on + strand at 1975.179 kb on + strand at 1975.179 kb on + strand at 1975.415 kb on - strand, within flhC at 1975.477 kb on + strand, within flhC at 1975.589 kb on + strand, within flhC at 1975.636 kb on + strand, within flhC at 1975.707 kb on + strand, within flhC at 1975.914 kb on - strand, within flhD at 1975.961 kb on - strand, within flhD at 1975.961 kb on - strand, within flhD at 1975.991 kb on + strand, within flhD at 1975.991 kb on - strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.120 kb on + strand, within flhD at 1976.120 kb on + strand, within flhD at 1976.138 kb on + strand, within flhD at 1976.146 kb on - strand, within flhD at 1976.149 kb on - strand, within flhD at 1976.149 kb on - strand, within flhD
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,973,279 - -3.5 1,973,279 - -2.4 1,973,443 - +0.2 1,973,443 - -0.7 1,973,577 - motB b1889 0.24 -1.6 1,973,595 - motB b1889 0.26 -3.1 1,973,697 + motB b1889 0.37 -4.8 1,973,846 - motB b1889 0.53 -2.6 1,973,850 - motB b1889 0.54 -2.1 1,974,089 - motB b1889 0.79 -2.7 1,974,089 - motB b1889 0.79 -2.4 1,974,197 + -3.8 1,974,226 + -2.3 1,974,233 + -0.6 1,974,233 + -2.0 1,974,414 + motA b1890 0.16 -2.1 1,974,854 - motA b1890 0.65 -2.6 1,974,934 + motA b1890 0.74 -2.5 1,974,934 + motA b1890 0.74 -4.5 1,975,040 + motA b1890 0.86 +0.9 1,975,092 + -0.5 1,975,093 - -2.4 1,975,173 + -2.9 1,975,179 + -2.5 1,975,179 + -1.9 1,975,415 - flhC b1891 0.22 -3.5 1,975,477 + flhC b1891 0.32 +0.1 1,975,589 + flhC b1891 0.52 +0.2 1,975,636 + flhC b1891 0.60 -3.1 1,975,707 + flhC b1891 0.72 +0.3 1,975,914 - flhD b1892 0.12 -0.1 1,975,961 - flhD b1892 0.26 -2.6 1,975,961 - flhD b1892 0.26 -2.9 1,975,991 + flhD b1892 0.34 -2.1 1,975,991 - flhD b1892 0.34 -4.0 1,976,072 + flhD b1892 0.57 +0.6 1,976,072 + flhD b1892 0.57 -2.6 1,976,072 + flhD b1892 0.57 -2.1 1,976,120 + flhD b1892 0.71 -0.4 1,976,120 + flhD b1892 0.71 -2.6 1,976,138 + flhD b1892 0.76 -2.9 1,976,146 - flhD b1892 0.78 -1.9 1,976,149 - flhD b1892 0.79 -2.5 1,976,149 - flhD b1892 0.79 -2.6
Or see this region's nucleotide sequence