Strain Fitness in Escherichia coli BW25113 around b1801

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeaU and yeaV are separated by 190 nucleotidesyeaV and yeaW are separated by 31 nucleotides b1800: yeaU - predicted dehydrogenase (NCBI), at 1,879,936 to 1,881,021 yeaU b1801: yeaV - predicted transporter (NCBI), at 1,881,212 to 1,882,657 yeaV b1802: yeaW - predicted 2Fe-2S cluster-containing protein (NCBI), at 1,882,689 to 1,883,813 yeaW Position (kb) 1881 1882 1883Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1880.373 kb on + strand, within yeaUat 1880.488 kb on + strand, within yeaUat 1880.488 kb on + strand, within yeaUat 1880.489 kb on - strand, within yeaUat 1880.516 kb on + strand, within yeaUat 1880.524 kb on - strand, within yeaUat 1880.535 kb on + strand, within yeaUat 1880.628 kb on - strand, within yeaUat 1880.642 kb on + strand, within yeaUat 1880.706 kb on + strand, within yeaUat 1880.787 kb on - strand, within yeaUat 1880.846 kb on + strand, within yeaUat 1880.881 kb on + strand, within yeaUat 1880.881 kb on + strand, within yeaUat 1880.890 kb on + strand, within yeaUat 1880.904 kb on - strand, within yeaUat 1880.929 kb on - strandat 1880.953 kb on + strandat 1880.956 kb on + strandat 1880.956 kb on + strandat 1880.977 kb on - strandat 1881.022 kb on + strandat 1881.053 kb on + strandat 1881.081 kb on + strandat 1881.172 kb on + strandat 1881.192 kb on - strandat 1881.206 kb on - strandat 1881.208 kb on - strandat 1881.243 kb on - strandat 1881.260 kb on - strandat 1881.298 kb on - strandat 1881.298 kb on - strandat 1881.303 kb on - strandat 1881.307 kb on + strandat 1881.307 kb on + strandat 1881.322 kb on + strandat 1881.322 kb on + strandat 1881.329 kb on + strandat 1881.474 kb on - strand, within yeaVat 1881.494 kb on - strand, within yeaVat 1881.494 kb on - strand, within yeaVat 1881.513 kb on - strand, within yeaVat 1881.715 kb on - strand, within yeaVat 1882.003 kb on + strand, within yeaVat 1882.003 kb on + strand, within yeaVat 1882.028 kb on - strand, within yeaVat 1882.190 kb on + strand, within yeaVat 1882.315 kb on + strand, within yeaVat 1882.315 kb on + strand, within yeaVat 1882.323 kb on - strand, within yeaVat 1882.323 kb on - strand, within yeaVat 1882.323 kb on - strand, within yeaVat 1882.324 kb on - strand, within yeaVat 1882.481 kb on + strand, within yeaVat 1882.635 kb on - strandat 1882.679 kb on + strandat 1882.679 kb on + strandat 1882.769 kb on + strandat 1883.061 kb on + strand, within yeaWat 1883.116 kb on - strand, within yeaWat 1883.116 kb on - strand, within yeaWat 1883.298 kb on - strand, within yeaWat 1883.298 kb on - strand, within yeaWat 1883.458 kb on - strand, within yeaWat 1883.503 kb on + strand, within yeaWat 1883.656 kb on - strand, within yeaW

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,880,373 + yeaU b1800 0.40 +1.4
1,880,488 + yeaU b1800 0.51 -0.6
1,880,488 + yeaU b1800 0.51 +1.2
1,880,489 - yeaU b1800 0.51 +0.4
1,880,516 + yeaU b1800 0.53 -1.0
1,880,524 - yeaU b1800 0.54 -0.3
1,880,535 + yeaU b1800 0.55 +1.8
1,880,628 - yeaU b1800 0.64 +0.2
1,880,642 + yeaU b1800 0.65 +0.0
1,880,706 + yeaU b1800 0.71 +0.6
1,880,787 - yeaU b1800 0.78 +0.5
1,880,846 + yeaU b1800 0.84 +1.8
1,880,881 + yeaU b1800 0.87 +0.8
1,880,881 + yeaU b1800 0.87 +0.2
1,880,890 + yeaU b1800 0.88 +1.8
1,880,904 - yeaU b1800 0.89 -0.3
1,880,929 - -1.3
1,880,953 + +1.0
1,880,956 + -0.2
1,880,956 + -2.6
1,880,977 - +0.3
1,881,022 + -0.2
1,881,053 + -3.2
1,881,081 + -0.5
1,881,172 + -1.5
1,881,192 - +1.9
1,881,206 - +1.2
1,881,208 - -1.7
1,881,243 - +2.1
1,881,260 - -2.0
1,881,298 - +0.1
1,881,298 - -0.3
1,881,303 - -0.7
1,881,307 + -0.3
1,881,307 + +1.7
1,881,322 + +0.0
1,881,322 + -1.3
1,881,329 + -1.9
1,881,474 - yeaV b1801 0.18 -0.6
1,881,494 - yeaV b1801 0.20 +2.0
1,881,494 - yeaV b1801 0.20 -1.5
1,881,513 - yeaV b1801 0.21 +0.0
1,881,715 - yeaV b1801 0.35 +1.4
1,882,003 + yeaV b1801 0.55 -1.0
1,882,003 + yeaV b1801 0.55 +1.1
1,882,028 - yeaV b1801 0.56 -1.8
1,882,190 + yeaV b1801 0.68 -1.7
1,882,315 + yeaV b1801 0.76 +1.0
1,882,315 + yeaV b1801 0.76 -0.5
1,882,323 - yeaV b1801 0.77 +0.0
1,882,323 - yeaV b1801 0.77 +1.8
1,882,323 - yeaV b1801 0.77 -0.5
1,882,324 - yeaV b1801 0.77 +0.5
1,882,481 + yeaV b1801 0.88 +0.1
1,882,635 - +0.0
1,882,679 + -0.4
1,882,679 + -0.8
1,882,769 + -0.6
1,883,061 + yeaW b1802 0.33 -1.4
1,883,116 - yeaW b1802 0.38 -1.6
1,883,116 - yeaW b1802 0.38 +0.2
1,883,298 - yeaW b1802 0.54 +0.9
1,883,298 - yeaW b1802 0.54 -0.1
1,883,458 - yeaW b1802 0.68 -1.2
1,883,503 + yeaW b1802 0.72 -1.9
1,883,656 - yeaW b1802 0.86 -0.3

Or see this region's nucleotide sequence