Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhI and b1644 are separated by 2 nucleotides b1644 and ydhK overlap by 1 nucleotides ydhK and sodC are separated by 0 nucleotides sodC and ydhF are separated by 80 nucleotides
b1643: ydhI - predicted inner membrane protein (NCBI), at 1,719,049 to 1,719,285
ydhI
b1644: b1644 - putative membrane protein (VIMSS), at 1,719,288 to 1,720,145
b1644
b1645: ydhK - conserved inner membrane protein (NCBI), at 1,720,145 to 1,722,157
ydhK
b1646: sodC - superoxide dismutase, Cu, Zn (NCBI), at 1,722,158 to 1,722,679
sodC
b1647: ydhF - predicted oxidoreductase (NCBI), at 1,722,760 to 1,723,656
ydhF
Position (kb) 1720
1721
1722
1723 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1719.163 kb on + strand, within ydhI at 1719.171 kb on - strand, within ydhI at 1719.171 kb on - strand, within ydhI at 1719.190 kb on + strand, within ydhI at 1719.211 kb on + strand, within ydhI at 1719.221 kb on - strand, within ydhI at 1719.559 kb on - strand, within b1644 at 1719.779 kb on + strand, within b1644 at 1719.779 kb on + strand, within b1644 at 1719.786 kb on - strand, within b1644 at 1719.786 kb on - strand, within b1644 at 1719.832 kb on - strand, within b1644 at 1719.832 kb on - strand, within b1644 at 1720.086 kb on + strand at 1720.196 kb on - strand at 1720.263 kb on + strand at 1720.263 kb on + strand at 1720.388 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.466 kb on + strand, within ydhK at 1720.520 kb on + strand, within ydhK at 1720.587 kb on - strand, within ydhK at 1720.587 kb on - strand, within ydhK at 1720.615 kb on + strand, within ydhK at 1720.741 kb on + strand, within ydhK at 1721.132 kb on + strand, within ydhK at 1721.142 kb on - strand, within ydhK at 1721.142 kb on - strand, within ydhK at 1721.170 kb on - strand, within ydhK at 1721.197 kb on + strand, within ydhK at 1721.225 kb on - strand, within ydhK at 1721.225 kb on - strand, within ydhK at 1721.272 kb on + strand, within ydhK at 1721.272 kb on + strand, within ydhK at 1721.272 kb on + strand, within ydhK at 1721.338 kb on + strand, within ydhK at 1721.343 kb on - strand, within ydhK at 1721.676 kb on + strand, within ydhK at 1721.722 kb on + strand, within ydhK at 1721.722 kb on + strand, within ydhK at 1721.762 kb on - strand, within ydhK at 1721.805 kb on - strand, within ydhK at 1721.805 kb on - strand, within ydhK at 1721.877 kb on - strand, within ydhK at 1721.877 kb on - strand, within ydhK at 1721.941 kb on + strand, within ydhK at 1722.103 kb on - strand at 1722.176 kb on + strand at 1722.692 kb on - strand at 1722.692 kb on - strand at 1722.868 kb on - strand, within ydhF at 1722.868 kb on - strand, within ydhF at 1722.904 kb on - strand, within ydhF at 1722.904 kb on - strand, within ydhF at 1723.132 kb on + strand, within ydhF at 1723.138 kb on - strand, within ydhF
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,719,163 + ydhI b1643 0.48 +0.2 1,719,171 - ydhI b1643 0.51 +1.9 1,719,171 - ydhI b1643 0.51 +2.1 1,719,190 + ydhI b1643 0.59 -0.3 1,719,211 + ydhI b1643 0.68 -0.2 1,719,221 - ydhI b1643 0.73 +2.9 1,719,559 - b1644 b1644 0.32 +1.3 1,719,779 + b1644 b1644 0.57 +1.1 1,719,779 + b1644 b1644 0.57 +0.1 1,719,786 - b1644 b1644 0.58 -0.4 1,719,786 - b1644 b1644 0.58 -0.9 1,719,832 - b1644 b1644 0.63 +0.6 1,719,832 - b1644 b1644 0.63 +2.3 1,720,086 + +0.7 1,720,196 - +0.5 1,720,263 + +0.4 1,720,263 + -0.1 1,720,388 + ydhK b1645 0.12 +0.9 1,720,466 + ydhK b1645 0.16 +0.0 1,720,466 + ydhK b1645 0.16 -1.1 1,720,466 + ydhK b1645 0.16 +1.6 1,720,466 + ydhK b1645 0.16 +2.3 1,720,520 + ydhK b1645 0.19 +0.1 1,720,587 - ydhK b1645 0.22 +1.5 1,720,587 - ydhK b1645 0.22 +1.2 1,720,615 + ydhK b1645 0.23 +0.5 1,720,741 + ydhK b1645 0.30 -0.3 1,721,132 + ydhK b1645 0.49 +2.5 1,721,142 - ydhK b1645 0.50 +2.8 1,721,142 - ydhK b1645 0.50 -0.1 1,721,170 - ydhK b1645 0.51 -2.9 1,721,197 + ydhK b1645 0.52 +0.9 1,721,225 - ydhK b1645 0.54 -1.0 1,721,225 - ydhK b1645 0.54 +1.0 1,721,272 + ydhK b1645 0.56 +1.1 1,721,272 + ydhK b1645 0.56 +0.9 1,721,272 + ydhK b1645 0.56 -1.1 1,721,338 + ydhK b1645 0.59 +0.4 1,721,343 - ydhK b1645 0.60 -0.2 1,721,676 + ydhK b1645 0.76 +0.5 1,721,722 + ydhK b1645 0.78 -0.9 1,721,722 + ydhK b1645 0.78 +3.2 1,721,762 - ydhK b1645 0.80 +0.4 1,721,805 - ydhK b1645 0.82 -0.1 1,721,805 - ydhK b1645 0.82 +2.0 1,721,877 - ydhK b1645 0.86 -0.1 1,721,877 - ydhK b1645 0.86 +1.1 1,721,941 + ydhK b1645 0.89 -0.6 1,722,103 - +0.8 1,722,176 + +0.2 1,722,692 - +2.6 1,722,692 - -1.9 1,722,868 - ydhF b1647 0.12 -1.5 1,722,868 - ydhF b1647 0.12 +3.4 1,722,904 - ydhF b1647 0.16 -0.2 1,722,904 - ydhF b1647 0.16 -1.2 1,723,132 + ydhF b1647 0.41 -0.1 1,723,138 - ydhF b1647 0.42 +1.5
Or see this region's nucleotide sequence