Strain Fitness in Escherichia coli BW25113 around b1303

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuE and pspF are separated by 119 nucleotidespspF and pspA are separated by 166 nucleotidespspA and pspB are separated by 53 nucleotides b1302: puuE - GABA aminotransferase, PLP-dependent (NCBI), at 1,363,574 to 1,364,839 puuE b1303: pspF - psp operon transcriptional activator (VIMSS), at 1,364,959 to 1,365,936 pspF b1304: pspA - regulatory protein for phage-shock-protein operon (NCBI), at 1,366,103 to 1,366,771 pspA b1305: pspB - phage shock protein B (NCBI), at 1,366,825 to 1,367,049 pspB Position (kb) 1364 1365 1366Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1363.993 kb on + strand, within puuEat 1364.468 kb on + strand, within puuEat 1364.619 kb on + strand, within puuEat 1364.739 kb on - strandat 1364.887 kb on - strandat 1364.951 kb on - strandat 1364.994 kb on + strandat 1365.094 kb on - strand, within pspFat 1365.094 kb on - strand, within pspFat 1365.177 kb on + strand, within pspFat 1365.234 kb on - strand, within pspFat 1365.275 kb on + strand, within pspFat 1365.299 kb on - strand, within pspFat 1365.299 kb on - strand, within pspFat 1365.402 kb on + strand, within pspFat 1365.559 kb on - strand, within pspFat 1365.559 kb on - strand, within pspFat 1365.591 kb on + strand, within pspFat 1365.850 kb on + strandat 1366.212 kb on + strand, within pspAat 1366.230 kb on - strand, within pspAat 1366.244 kb on + strand, within pspAat 1366.373 kb on - strand, within pspAat 1366.560 kb on + strand, within pspAat 1366.628 kb on + strand, within pspAat 1366.818 kb on - strandat 1366.840 kb on - strandat 1366.896 kb on + strand, within pspBat 1366.931 kb on + strand, within pspB

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,363,993 + puuE b1302 0.33 +0.7
1,364,468 + puuE b1302 0.71 +1.4
1,364,619 + puuE b1302 0.83 +1.7
1,364,739 - +0.2
1,364,887 - +0.1
1,364,951 - +0.9
1,364,994 + +2.0
1,365,094 - pspF b1303 0.14 +1.1
1,365,094 - pspF b1303 0.14 +1.5
1,365,177 + pspF b1303 0.22 +0.2
1,365,234 - pspF b1303 0.28 +0.7
1,365,275 + pspF b1303 0.32 -2.7
1,365,299 - pspF b1303 0.35 -0.9
1,365,299 - pspF b1303 0.35 +0.6
1,365,402 + pspF b1303 0.45 +0.4
1,365,559 - pspF b1303 0.61 -0.1
1,365,559 - pspF b1303 0.61 +0.2
1,365,591 + pspF b1303 0.65 +3.0
1,365,850 + +0.8
1,366,212 + pspA b1304 0.16 +0.3
1,366,230 - pspA b1304 0.19 +0.6
1,366,244 + pspA b1304 0.21 +1.5
1,366,373 - pspA b1304 0.40 +0.6
1,366,560 + pspA b1304 0.68 -0.1
1,366,628 + pspA b1304 0.78 -1.7
1,366,818 - +1.6
1,366,840 - +0.3
1,366,896 + pspB b1305 0.32 -0.1
1,366,931 + pspB b1305 0.47 +2.4

Or see this region's nucleotide sequence