Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malZ and yajB are separated by 4 nucleotides yajB and queA are separated by 92 nucleotides queA and tgt are separated by 55 nucleotides
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
b0404: yajB - hypothetical protein (NCBI), at 423,561 to 424,142
yajB
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
b0406: tgt - queuine tRNA-ribosyltransferase (NCBI), at 425,361 to 426,488
tgt
Position (kb) 424
425
426 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 423.283 kb on - strand, within malZ at 423.401 kb on - strand at 423.404 kb on - strand at 423.404 kb on - strand at 423.445 kb on - strand at 423.512 kb on + strand at 423.548 kb on - strand at 423.548 kb on - strand at 423.711 kb on + strand, within yajB at 423.711 kb on + strand, within yajB at 423.909 kb on + strand, within yajB at 423.909 kb on + strand at 423.923 kb on + strand, within yajB at 423.996 kb on + strand, within yajB at 424.011 kb on - strand, within yajB at 424.112 kb on + strand at 424.121 kb on - strand at 424.121 kb on - strand at 424.232 kb on + strand at 424.232 kb on + strand at 424.280 kb on + strand at 424.280 kb on + strand at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA at 424.612 kb on + strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.657 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.922 kb on - strand, within queA at 425.147 kb on - strand, within queA at 425.147 kb on - strand, within queA at 425.300 kb on + strand at 425.300 kb on + strand at 425.436 kb on - strand at 425.470 kb on + strand at 425.700 kb on - strand, within tgt at 425.700 kb on - strand, within tgt at 425.852 kb on - strand, within tgt at 425.914 kb on + strand, within tgt at 425.945 kb on - strand, within tgt at 425.945 kb on - strand at 425.964 kb on + strand, within tgt at 426.030 kb on - strand, within tgt at 426.030 kb on - strand, within tgt at 426.035 kb on - strand, within tgt at 426.035 kb on - strand, within tgt at 426.230 kb on + strand, within tgt at 426.230 kb on + strand, within tgt at 426.241 kb on + strand, within tgt at 426.249 kb on - strand, within tgt at 426.259 kb on - strand, within tgt at 426.277 kb on - strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 423,283 - malZ b0403 0.85 +0.6 423,401 - +0.3 423,404 - +1.0 423,404 - -0.6 423,445 - +0.4 423,512 + -0.8 423,548 - +0.2 423,548 - -1.5 423,711 + yajB b0404 0.26 +0.5 423,711 + yajB b0404 0.26 +0.8 423,909 + yajB b0404 0.60 +0.2 423,909 + +1.2 423,923 + yajB b0404 0.62 -0.7 423,996 + yajB b0404 0.75 +1.5 424,011 - yajB b0404 0.77 +0.9 424,112 + +3.4 424,121 - -1.9 424,121 - +1.8 424,232 + -1.6 424,232 + -1.0 424,280 + -1.8 424,280 + -1.6 424,484 - queA b0405 0.23 -0.7 424,484 - queA b0405 0.23 -2.6 424,612 + queA b0405 0.35 -2.1 424,651 - queA b0405 0.39 -0.1 424,651 - queA b0405 0.39 -2.4 424,651 - queA b0405 0.39 -3.9 424,657 + queA b0405 0.39 -0.8 424,668 + queA b0405 0.40 -1.0 424,668 + queA b0405 0.40 -2.1 424,922 - queA b0405 0.64 -1.7 425,147 - queA b0405 0.85 -2.3 425,147 - queA b0405 0.85 -3.8 425,300 + -2.0 425,300 + +1.5 425,436 - +2.0 425,470 + -1.2 425,700 - tgt b0406 0.30 -1.9 425,700 - tgt b0406 0.30 -1.7 425,852 - tgt b0406 0.44 -2.0 425,914 + tgt b0406 0.49 -2.4 425,945 - tgt b0406 0.52 -1.4 425,945 - +0.9 425,964 + tgt b0406 0.53 -2.6 426,030 - tgt b0406 0.59 -1.3 426,030 - tgt b0406 0.59 +1.5 426,035 - tgt b0406 0.60 -0.9 426,035 - tgt b0406 0.60 -0.9 426,230 + tgt b0406 0.77 -1.5 426,230 + tgt b0406 0.77 +0.4 426,241 + tgt b0406 0.78 -1.8 426,249 - tgt b0406 0.79 -2.2 426,259 - tgt b0406 0.80 -4.1 426,277 - tgt b0406 0.81 -3.1
Or see this region's nucleotide sequence