Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frvA and yiiL are separated by 300 nucleotides yiiL and rhaD are separated by 9 nucleotides rhaD and rhaA are separated by 450 nucleotides
b3900: frvA - predicted enzyme IIA component of PTS (NCBI), at 4,090,400 to 4,090,846
frvA
b3901: yiiL - L-rhamnose mutarotase (NCBI), at 4,091,147 to 4,091,461
yiiL
b3902: rhaD - rhamnulose-1-phosphate aldolase (NCBI), at 4,091,471 to 4,092,295
rhaD
b3903: rhaA - L-rhamnose isomerase (NCBI), at 4,092,746 to 4,094,005
rhaA
Position (kb)
4091
4092
4093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4090.554 kb on + strand, within frvA at 4090.554 kb on + strand, within frvA at 4090.554 kb on + strand, within frvA at 4090.554 kb on + strand, within frvA at 4090.561 kb on - strand, within frvA at 4090.561 kb on - strand, within frvA at 4090.591 kb on + strand, within frvA at 4090.591 kb on + strand, within frvA at 4090.645 kb on + strand, within frvA at 4090.655 kb on + strand, within frvA at 4090.660 kb on + strand, within frvA at 4090.667 kb on - strand, within frvA at 4090.670 kb on + strand, within frvA at 4090.679 kb on + strand, within frvA at 4090.679 kb on + strand, within frvA at 4090.681 kb on + strand, within frvA at 4090.706 kb on + strand, within frvA at 4090.708 kb on + strand, within frvA at 4090.708 kb on + strand, within frvA at 4090.711 kb on + strand, within frvA at 4090.714 kb on + strand, within frvA at 4090.719 kb on + strand, within frvA at 4090.719 kb on + strand, within frvA at 4090.719 kb on + strand, within frvA at 4090.729 kb on + strand, within frvA at 4090.729 kb on + strand, within frvA at 4090.729 kb on + strand, within frvA at 4090.729 kb on + strand, within frvA at 4090.732 kb on - strand, within frvA at 4090.764 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.767 kb on + strand, within frvA at 4090.777 kb on + strand, within frvA at 4090.777 kb on + strand, within frvA at 4090.778 kb on + strand, within frvA at 4090.778 kb on + strand, within frvA at 4090.788 kb on + strand, within frvA at 4090.788 kb on + strand, within frvA at 4090.788 kb on + strand, within frvA at 4090.802 kb on + strand at 4090.809 kb on + strand at 4090.821 kb on + strand at 4090.821 kb on + strand at 4090.821 kb on + strand at 4090.826 kb on + strand at 4090.829 kb on - strand at 4090.831 kb on + strand at 4090.831 kb on + strand at 4090.831 kb on + strand at 4090.831 kb on + strand at 4090.831 kb on + strand at 4090.831 kb on + strand at 4090.837 kb on + strand at 4090.837 kb on + strand at 4090.842 kb on + strand at 4090.842 kb on + strand at 4090.879 kb on + strand at 4090.879 kb on + strand at 4090.892 kb on + strand at 4090.895 kb on + strand at 4090.899 kb on + strand at 4090.900 kb on - strand at 4090.900 kb on - strand at 4090.902 kb on + strand at 4090.903 kb on - strand at 4090.910 kb on + strand at 4090.918 kb on - strand at 4090.967 kb on + strand at 4090.967 kb on + strand at 4090.967 kb on + strand at 4090.969 kb on + strand at 4090.970 kb on + strand at 4090.970 kb on + strand at 4090.972 kb on + strand at 4090.972 kb on + strand at 4091.136 kb on - strand at 4091.139 kb on - strand at 4091.162 kb on - strand at 4091.173 kb on + strand at 4091.173 kb on + strand at 4091.178 kb on + strand at 4091.181 kb on - strand, within yiiL at 4091.181 kb on - strand, within yiiL at 4091.181 kb on - strand, within yiiL at 4091.181 kb on - strand, within yiiL at 4091.181 kb on - strand, within yiiL at 4091.183 kb on - strand, within yiiL at 4091.183 kb on - strand, within yiiL at 4091.183 kb on - strand, within yiiL at 4091.186 kb on - strand, within yiiL at 4091.186 kb on - strand, within yiiL at 4091.186 kb on - strand, within yiiL at 4091.186 kb on - strand, within yiiL at 4091.188 kb on - strand, within yiiL at 4091.188 kb on - strand, within yiiL at 4091.190 kb on + strand, within yiiL at 4091.190 kb on - strand, within yiiL at 4091.190 kb on - strand, within yiiL at 4091.190 kb on - strand, within yiiL at 4091.190 kb on - strand, within yiiL at 4091.192 kb on + strand, within yiiL at 4091.200 kb on - strand, within yiiL at 4091.201 kb on - strand, within yiiL at 4091.201 kb on - strand, within yiiL at 4091.201 kb on - strand, within yiiL at 4091.202 kb on - strand, within yiiL at 4091.203 kb on - strand, within yiiL at 4091.207 kb on - strand, within yiiL at 4091.212 kb on - strand, within yiiL at 4091.215 kb on - strand, within yiiL at 4091.226 kb on - strand, within yiiL at 4091.226 kb on - strand, within yiiL at 4091.253 kb on + strand, within yiiL at 4091.256 kb on - strand, within yiiL at 4091.261 kb on - strand, within yiiL at 4091.270 kb on - strand, within yiiL at 4091.301 kb on - strand, within yiiL at 4091.320 kb on - strand, within yiiL at 4091.350 kb on - strand, within yiiL at 4091.350 kb on - strand, within yiiL at 4091.350 kb on - strand, within yiiL at 4091.370 kb on + strand, within yiiL at 4091.380 kb on - strand, within yiiL at 4091.380 kb on - strand, within yiiL at 4091.380 kb on - strand, within yiiL at 4091.391 kb on + strand, within yiiL at 4091.391 kb on + strand, within yiiL at 4091.399 kb on - strand, within yiiL at 4091.403 kb on + strand, within yiiL at 4091.411 kb on - strand, within yiiL at 4091.411 kb on - strand, within yiiL at 4091.427 kb on + strand, within yiiL at 4091.449 kb on - strand at 4091.464 kb on - strand at 4091.479 kb on - strand at 4091.524 kb on + strand at 4091.524 kb on + strand at 4091.548 kb on - strand at 4091.576 kb on - strand, within rhaD at 4091.587 kb on - strand, within rhaD at 4091.656 kb on - strand, within rhaD at 4091.660 kb on + strand, within rhaD at 4091.660 kb on + strand, within rhaD at 4091.675 kb on - strand, within rhaD at 4091.704 kb on - strand, within rhaD at 4091.704 kb on - strand, within rhaD at 4091.735 kb on - strand, within rhaD at 4091.739 kb on + strand, within rhaD at 4091.739 kb on + strand, within rhaD at 4091.747 kb on - strand, within rhaD at 4091.747 kb on - strand, within rhaD at 4091.795 kb on - strand, within rhaD at 4091.795 kb on - strand, within rhaD at 4091.809 kb on - strand, within rhaD at 4091.809 kb on - strand, within rhaD at 4091.838 kb on + strand, within rhaD at 4091.873 kb on + strand, within rhaD at 4091.881 kb on - strand, within rhaD at 4091.881 kb on - strand, within rhaD at 4091.881 kb on - strand, within rhaD at 4091.921 kb on + strand, within rhaD at 4091.921 kb on + strand, within rhaD at 4091.959 kb on - strand, within rhaD
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,090,554 + frvA b3900 0.34 -1.4 4,090,554 + frvA b3900 0.34 -0.5 4,090,554 + frvA b3900 0.34 +1.2 4,090,554 + frvA b3900 0.34 -0.2 4,090,561 - frvA b3900 0.36 -1.6 4,090,561 - frvA b3900 0.36 +1.2 4,090,591 + frvA b3900 0.43 +0.1 4,090,591 + frvA b3900 0.43 +0.3 4,090,645 + frvA b3900 0.55 +0.2 4,090,655 + frvA b3900 0.57 +0.8 4,090,660 + frvA b3900 0.58 +0.3 4,090,667 - frvA b3900 0.60 +0.7 4,090,670 + frvA b3900 0.60 +0.5 4,090,679 + frvA b3900 0.62 -0.1 4,090,679 + frvA b3900 0.62 +1.1 4,090,681 + frvA b3900 0.63 -0.6 4,090,706 + frvA b3900 0.68 -0.6 4,090,708 + frvA b3900 0.69 -0.7 4,090,708 + frvA b3900 0.69 -2.1 4,090,711 + frvA b3900 0.70 +0.6 4,090,714 + frvA b3900 0.70 -0.7 4,090,719 + frvA b3900 0.71 -2.1 4,090,719 + frvA b3900 0.71 -0.9 4,090,719 + frvA b3900 0.71 -1.2 4,090,729 + frvA b3900 0.74 -0.6 4,090,729 + frvA b3900 0.74 +2.3 4,090,729 + frvA b3900 0.74 +0.5 4,090,729 + frvA b3900 0.74 +1.2 4,090,732 - frvA b3900 0.74 +0.1 4,090,764 + frvA b3900 0.81 -1.2 4,090,767 + frvA b3900 0.82 +0.9 4,090,767 + frvA b3900 0.82 -0.3 4,090,767 + frvA b3900 0.82 -2.3 4,090,767 + frvA b3900 0.82 -2.0 4,090,767 + frvA b3900 0.82 +1.0 4,090,767 + frvA b3900 0.82 -1.5 4,090,767 + frvA b3900 0.82 +1.2 4,090,767 + frvA b3900 0.82 +2.6 4,090,767 + frvA b3900 0.82 -0.4 4,090,777 + frvA b3900 0.84 -0.2 4,090,777 + frvA b3900 0.84 -0.2 4,090,778 + frvA b3900 0.85 -0.4 4,090,778 + frvA b3900 0.85 -0.9 4,090,788 + frvA b3900 0.87 +0.3 4,090,788 + frvA b3900 0.87 -1.3 4,090,788 + frvA b3900 0.87 -0.7 4,090,802 + +0.6 4,090,809 + -0.6 4,090,821 + -0.1 4,090,821 + -0.3 4,090,821 + -1.2 4,090,826 + +0.6 4,090,829 - +1.1 4,090,831 + -1.5 4,090,831 + -0.2 4,090,831 + +0.0 4,090,831 + -1.0 4,090,831 + -0.4 4,090,831 + -0.8 4,090,837 + +0.3 4,090,837 + +1.4 4,090,842 + +0.1 4,090,842 + +0.1 4,090,879 + -0.4 4,090,879 + +0.7 4,090,892 + +0.2 4,090,895 + +0.0 4,090,899 + +0.7 4,090,900 - +0.3 4,090,900 - -1.1 4,090,902 + +0.3 4,090,903 - -0.3 4,090,910 + +0.7 4,090,918 - -0.5 4,090,967 + +0.9 4,090,967 + -1.1 4,090,967 + -1.4 4,090,969 + -1.5 4,090,970 + +0.7 4,090,970 + +1.7 4,090,972 + +0.2 4,090,972 + +0.1 4,091,136 - +0.2 4,091,139 - -2.2 4,091,162 - -0.1 4,091,173 + -2.7 4,091,173 + +1.3 4,091,178 + +0.1 4,091,181 - yiiL b3901 0.11 -2.0 4,091,181 - yiiL b3901 0.11 +0.9 4,091,181 - yiiL b3901 0.11 -0.4 4,091,181 - yiiL b3901 0.11 -0.8 4,091,181 - yiiL b3901 0.11 -0.3 4,091,183 - yiiL b3901 0.11 +0.2 4,091,183 - yiiL b3901 0.11 +3.2 4,091,183 - yiiL b3901 0.11 +1.0 4,091,186 - yiiL b3901 0.12 -1.2 4,091,186 - yiiL b3901 0.12 +1.7 4,091,186 - yiiL b3901 0.12 +1.5 4,091,186 - yiiL b3901 0.12 +0.4 4,091,188 - yiiL b3901 0.13 +1.0 4,091,188 - yiiL b3901 0.13 -0.8 4,091,190 + yiiL b3901 0.14 +1.5 4,091,190 - yiiL b3901 0.14 +0.8 4,091,190 - yiiL b3901 0.14 -1.1 4,091,190 - yiiL b3901 0.14 +1.2 4,091,190 - yiiL b3901 0.14 -1.6 4,091,192 + yiiL b3901 0.14 -0.8 4,091,200 - yiiL b3901 0.17 -1.7 4,091,201 - yiiL b3901 0.17 +0.6 4,091,201 - yiiL b3901 0.17 +0.6 4,091,201 - yiiL b3901 0.17 -0.0 4,091,202 - yiiL b3901 0.17 -1.8 4,091,203 - yiiL b3901 0.18 +0.2 4,091,207 - yiiL b3901 0.19 -0.4 4,091,212 - yiiL b3901 0.21 +1.4 4,091,215 - yiiL b3901 0.22 +0.2 4,091,226 - yiiL b3901 0.25 -1.5 4,091,226 - yiiL b3901 0.25 +2.3 4,091,253 + yiiL b3901 0.34 -2.0 4,091,256 - yiiL b3901 0.35 -0.4 4,091,261 - yiiL b3901 0.36 +0.4 4,091,270 - yiiL b3901 0.39 -2.5 4,091,301 - yiiL b3901 0.49 +0.0 4,091,320 - yiiL b3901 0.55 -2.1 4,091,350 - yiiL b3901 0.64 +1.0 4,091,350 - yiiL b3901 0.64 -2.1 4,091,350 - yiiL b3901 0.64 +0.2 4,091,370 + yiiL b3901 0.71 +1.0 4,091,380 - yiiL b3901 0.74 -1.5 4,091,380 - yiiL b3901 0.74 -0.4 4,091,380 - yiiL b3901 0.74 +2.6 4,091,391 + yiiL b3901 0.77 -0.2 4,091,391 + yiiL b3901 0.77 +1.0 4,091,399 - yiiL b3901 0.80 -2.9 4,091,403 + yiiL b3901 0.81 +1.9 4,091,411 - yiiL b3901 0.84 +1.9 4,091,411 - yiiL b3901 0.84 +1.1 4,091,427 + yiiL b3901 0.89 +1.5 4,091,449 - -1.5 4,091,464 - +0.4 4,091,479 - -0.5 4,091,524 + +0.9 4,091,524 + -1.7 4,091,548 - +2.1 4,091,576 - rhaD b3902 0.13 +0.8 4,091,587 - rhaD b3902 0.14 -1.0 4,091,656 - rhaD b3902 0.22 -0.2 4,091,660 + rhaD b3902 0.23 -1.8 4,091,660 + rhaD b3902 0.23 -1.4 4,091,675 - rhaD b3902 0.25 -2.4 4,091,704 - rhaD b3902 0.28 -0.3 4,091,704 - rhaD b3902 0.28 -1.4 4,091,735 - rhaD b3902 0.32 +0.4 4,091,739 + rhaD b3902 0.32 +0.2 4,091,739 + rhaD b3902 0.32 +0.1 4,091,747 - rhaD b3902 0.33 -0.6 4,091,747 - rhaD b3902 0.33 -0.3 4,091,795 - rhaD b3902 0.39 -1.5 4,091,795 - rhaD b3902 0.39 +0.7 4,091,809 - rhaD b3902 0.41 +1.5 4,091,809 - rhaD b3902 0.41 -0.6 4,091,838 + rhaD b3902 0.44 -0.5 4,091,873 + rhaD b3902 0.49 -1.3 4,091,881 - rhaD b3902 0.50 -1.9 4,091,881 - rhaD b3902 0.50 -0.1 4,091,881 - rhaD b3902 0.50 +0.3 4,091,921 + rhaD b3902 0.55 +0.2 4,091,921 + rhaD b3902 0.55 +0.8 4,091,959 - rhaD b3902 0.59 +0.0
Or see this region's nucleotide sequence