Strain Fitness in Escherichia coli BW25113 around b3379

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
3,504,747	+	yhfT	b3377	0.53	-0.9
3,504,747	+	yhfT	b3377	0.53	-2.9
3,504,763	-	yhfT	b3377	0.54	-0.2
3,504,774	+	yhfT	b3377	0.55	+0.3
3,504,774	+	yhfT	b3377	0.55	-0.6
3,504,837	-	yhfT	b3377	0.60	+1.5
3,504,855	+	yhfT	b3377	0.61	+4.4
3,504,855	+	yhfT	b3377	0.61	+0.0
3,504,871	-	yhfT	b3377	0.63	+0.1
3,504,871	-	yhfT	b3377	0.63	+1.9
3,504,887	-	yhfT	b3377	0.64	-0.0
3,504,887	-	yhfT	b3377	0.64	-0.4
3,504,887	-	yhfT	b3377	0.64	-2.5
3,504,932	+	yhfT	b3377	0.67	-2.4
3,504,932	+				+4.2
3,504,985	+	yhfT	b3377	0.71	+1.0
3,504,985	+	yhfT	b3377	0.71	-1.5
3,505,002	+	yhfT	b3377	0.73	+2.3
3,505,025	+	yhfT	b3377	0.74	+2.1
3,505,035	+	yhfT	b3377	0.75	-1.7
3,505,043	-	yhfT	b3377	0.76	-1.3
3,505,043	-	yhfT	b3377	0.76	-0.0
3,505,043	-	yhfT	b3377	0.76	-2.2
3,505,044	+	yhfT	b3377	0.76	+0.3
3,505,044	+	yhfT	b3377	0.76	-1.7
3,505,088	-	yhfT	b3377	0.79	+0.6
3,505,088	-	yhfT	b3377	0.79	+1.1
3,505,124	+	yhfT	b3377	0.82	+0.6
3,505,124	+	yhfT	b3377	0.82	+1.5
3,505,124	+	yhfT	b3377	0.82	+0.8
3,505,124	+	yhfT	b3377	0.82	-0.2
3,505,127	-	yhfT	b3377	0.82	+0.2
3,505,132	-	yhfT	b3377	0.83	+1.1
3,505,151	+	yhfT	b3377	0.84	-0.3
3,505,193	+	yhfT	b3377	0.87	+0.2
3,505,207	+	yhfT	b3377	0.88	+0.2
3,505,232	-				-0.2
3,505,232	-				+1.2
3,505,234	+				+0.4
3,505,234	+				+1.1
3,505,236	-				+0.5
3,505,244	+				-1.9
3,505,328	+				+1.5
3,505,504	-	yhfU	b3378	0.38	-0.5
3,505,556	-	yhfU	b3378	0.53	-1.3
3,505,556	-	yhfU	b3378	0.53	-1.9
3,505,560	-	yhfU	b3378	0.54	-0.6
3,505,571	-	yhfU	b3378	0.57	+0.2
3,505,652	+	yhfU	b3378	0.80	+1.5
3,505,652	+	yhfU	b3378	0.80	-0.4
3,505,659	-	yhfU	b3378	0.82	+0.6
3,505,687	+	yhfU	b3378	0.90	-0.8
3,505,718	+				+3.3
3,505,724	+				-1.6
3,505,725	+				+0.7
3,505,734	-				+1.2
3,505,767	+				-2.3
3,505,767	+				+1.5
3,505,780	-				-0.9
3,505,787	+				-0.0
3,505,793	+				+0.2
3,505,793	+				-1.1
3,505,796	-				+0.7
3,505,895	+	php	b3379	0.18	-0.7
3,505,913	-	php	b3379	0.20	+0.3
3,505,992	-	php	b3379	0.29	+0.2
3,506,060	-	php	b3379	0.37	+0.4
3,506,060	-	php	b3379	0.37	-0.9
3,506,062	+	php	b3379	0.37	-0.6
3,506,165	+	php	b3379	0.49	-0.2
3,506,168	-	php	b3379	0.49	-0.8
3,506,168	-	php	b3379	0.49	+0.4
3,506,173	-	php	b3379	0.50	+0.8
3,506,238	-	php	b3379	0.57	-3.0
3,506,296	+	php	b3379	0.64	-1.5
3,506,296	+	php	b3379	0.64	-0.2
3,506,296	+	php	b3379	0.64	-1.8
3,506,313	+	php	b3379	0.66	+1.1
3,506,313	+	php	b3379	0.66	+0.5
3,506,320	-	php	b3379	0.67	+0.4
3,506,330	+	php	b3379	0.68	+0.2
3,506,371	+	php	b3379	0.72	-0.7
3,506,379	-	php	b3379	0.73	-1.9
3,506,379	-	php	b3379	0.73	-0.9
3,506,385	+	php	b3379	0.74	+0.5
3,506,458	+	php	b3379	0.82	+0.4
3,506,458	+	php	b3379	0.82	+0.6
3,506,469	-	php	b3379	0.84	-1.9
3,506,489	-	php	b3379	0.86	-1.7
3,506,489	-	php	b3379	0.86	-1.9
3,506,509	+	php	b3379	0.88	+0.1
3,506,558	+				+1.4
3,506,584	-				-0.7
3,506,594	-				+0.4
3,506,595	-				-2.4
3,506,697	+				+1.3
3,506,703	+				+0.6
3,506,707	-				+0.1
3,506,707	-				-2.5
3,506,715	+				+0.7
3,506,715	+				+1.5
3,506,734	+	yhfW	b3380	0.10	+0.2
3,506,734	+	yhfW	b3380	0.10	+1.0
3,506,772	-	yhfW	b3380	0.13	-0.5
3,506,792	+	yhfW	b3380	0.15	+1.6
3,506,792	+	yhfW	b3380	0.15	-2.9
3,506,813	-	yhfW	b3380	0.17	-1.4
3,506,813	-	yhfW	b3380	0.17	+0.6
3,506,858	+	yhfW	b3380	0.20	+1.4
3,506,885	-	yhfW	b3380	0.22	-0.9
3,506,950	+	yhfW	b3380	0.28	-1.2
3,506,976	+	yhfW	b3380	0.30	-2.6
3,507,017	-	yhfW	b3380	0.33	-0.6
3,507,017	-	yhfW	b3380	0.33	+0.6
3,507,078	+	yhfW	b3380	0.38	+0.6
3,507,078	+	yhfW	b3380	0.38	-2.3
3,507,089	-	yhfW	b3380	0.39	+0.0
3,507,089	-	yhfW	b3380	0.39	+0.3
3,507,094	+	yhfW	b3380	0.40	+1.3
3,507,108	-	yhfW	b3380	0.41	-1.6
3,507,155	+	yhfW	b3380	0.44	-1.4
3,507,155	+	yhfW	b3380	0.44	+0.3
3,507,157	+	yhfW	b3380	0.45	+2.2
3,507,169	+	yhfW	b3380	0.46	+1.0
3,507,169	+	yhfW	b3380	0.46	-2.6
3,507,189	+	yhfW	b3380	0.47	-2.8
3,507,205	+	yhfW	b3380	0.49	+0.5
3,507,213	-	yhfW	b3380	0.49	-2.2
3,507,242	+	yhfW	b3380	0.52	+1.8
3,507,242	+	yhfW	b3380	0.52	-1.3
3,507,274	-	yhfW	b3380	0.54	-3.1
3,507,286	-	yhfW	b3380	0.55	-1.0
3,507,300	+	yhfW	b3380	0.56	-1.8
3,507,300	+	yhfW	b3380	0.56	+0.4
3,507,300	+	yhfW	b3380	0.56	-1.0
3,507,308	-	yhfW	b3380	0.57	+0.1
3,507,308	-	yhfW	b3380	0.57	-1.1
3,507,310	+	yhfW	b3380	0.57	-0.4
3,507,327	-	yhfW	b3380	0.59	-3.7
3,507,343	+	yhfW	b3380	0.60	-2.7
3,507,386	-	yhfW	b3380	0.63	+0.2
3,507,459	-	yhfW	b3380	0.69	-0.4
3,507,472	-	yhfW	b3380	0.70	+1.0
3,507,472	-	yhfW	b3380	0.70	+0.9
3,507,525	-	yhfW	b3380	0.75	+0.7
3,507,525	-	yhfW	b3380	0.75	-2.6
3,507,574	+	yhfW	b3380	0.79	-1.8
3,507,574	+	yhfW	b3380	0.79	+1.6
3,507,607	+	yhfW	b3380	0.81	+1.1
3,507,607	+	yhfW	b3380	0.81	+0.5