Strain Fitness in Escherichia coli BW25113 around b3349

Experiment: outer cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	outer cut, LB soft agar motility assay
3,474,935	-	fkpA	b3347	0.38	-0.3
3,474,945	-	fkpA	b3347	0.39	-2.6
3,474,945	-	fkpA	b3347	0.39	-0.6
3,474,956	+	fkpA	b3347	0.40	-0.9
3,474,968	+	fkpA	b3347	0.42	-0.7
3,474,981	+	fkpA	b3347	0.43	+0.9
3,474,982	+	fkpA	b3347	0.43	+0.8
3,475,013	-	fkpA	b3347	0.47	+0.2
3,475,049	+	fkpA	b3347	0.52	+0.3
3,475,123	+	fkpA	b3347	0.61	-0.1
3,475,132	+	fkpA	b3347	0.62	-0.6
3,475,133	-	fkpA	b3347	0.62	+0.4
3,475,133	-	fkpA	b3347	0.62	+0.9
3,475,134	+	fkpA	b3347	0.62	+0.5
3,475,134	+	fkpA	b3347	0.62	-0.4
3,475,140	-	fkpA	b3347	0.63	+2.0
3,475,266	+	fkpA	b3347	0.78	+1.3
3,475,269	+	fkpA	b3347	0.79	+0.8
3,475,271	+	fkpA	b3347	0.79	-2.3
3,475,274	-	fkpA	b3347	0.79	-1.4
3,475,274	-	fkpA	b3347	0.79	-0.3
3,475,276	+	fkpA	b3347	0.80	-1.1
3,475,283	+	fkpA	b3347	0.80	+0.3
3,475,321	+	fkpA	b3347	0.85	-1.7
3,475,334	-				+4.8
3,475,344	+	fkpA	b3347	0.88	-0.8
3,475,344	+	fkpA	b3347	0.88	-0.4
3,475,381	+				-0.4
3,475,381	+				-0.1
3,475,381	+				+1.3
3,475,385	+				+0.1
3,475,385	+				+2.1
3,475,385	+				-2.0
3,475,385	+				-0.4
3,475,425	-				+0.9
3,475,425	-				-0.5
3,475,438	-				+1.0
3,475,459	+				-0.6
3,475,459	+				-0.9
3,475,469	+				-0.1
3,475,474	+				+2.6
3,475,489	-				-1.8
3,475,498	-				-2.3
3,475,506	-				-1.1
3,475,523	+				-1.8
3,475,531	-				-2.6
3,475,533	-				-1.2
3,475,533	-				-1.8
3,475,544	-				-0.7
3,475,705	-	slyX	b3348	0.20	-1.7
3,475,705	-	slyX	b3348	0.20	-0.2
3,475,732	+	slyX	b3348	0.32	-0.2
3,475,732	+	slyX	b3348	0.32	-0.0
3,475,745	-	slyX	b3348	0.38	-0.4
3,475,787	+	slyX	b3348	0.57	-1.5
3,475,787	+	slyX	b3348	0.57	-1.8
3,475,797	+	slyX	b3348	0.62	-1.2
3,475,797	+	slyX	b3348	0.62	-2.1
3,475,861	+				+0.9
3,475,874	+				-1.8
3,475,874	+				+0.2
3,475,939	-				-0.5
3,475,971	+				-0.0
3,475,991	-	slyD	b3349	0.10	+0.1
3,475,991	-	slyD	b3349	0.10	-0.4
3,476,000	-	slyD	b3349	0.12	-2.1
3,476,042	+				+1.0
3,476,042	+	slyD	b3349	0.19	-0.2
3,476,061	+	slyD	b3349	0.22	+0.3
3,476,061	+	slyD	b3349	0.22	-0.3
3,476,063	+	slyD	b3349	0.23	-1.3
3,476,063	+	slyD	b3349	0.23	-1.6
3,476,089	-	slyD	b3349	0.27	-1.1
3,476,089	-	slyD	b3349	0.27	+0.8
3,476,114	-	slyD	b3349	0.31	-1.0
3,476,143	+	slyD	b3349	0.36	+3.7
3,476,206	+	slyD	b3349	0.47	-1.6
3,476,212	-	slyD	b3349	0.48	-0.4
3,476,242	+	slyD	b3349	0.53	-1.3
3,476,286	+	slyD	b3349	0.60	-0.7
3,476,333	-	slyD	b3349	0.68	-0.0
3,476,378	-	slyD	b3349	0.76	-0.4
3,476,423	-	slyD	b3349	0.84	-1.7
3,476,423	-	slyD	b3349	0.84	+2.0
3,476,465	+				-2.2
3,476,473	-				-0.2
3,476,473	-				+1.5
3,476,479	+				-0.5
3,476,479	+				-0.1
3,476,485	-				+0.4
3,476,485	-				-1.6
3,476,522	-				-0.1
3,476,525	+				+0.6
3,476,525	+				+1.4
3,476,525	+				-1.5
3,476,525	+				+0.4
3,476,544	-				-0.5
3,476,544	-				-0.7
3,476,549	-				+0.8
3,476,612	-				+1.5
3,476,649	-	yheV	b4551	0.17	-0.2
3,476,768	-	yheV	b4551	0.77	+2.2
3,476,810	+				-0.5
3,476,853	-				+1.2
3,476,917	-				+0.7
3,476,917	-				+1.0
3,476,933	+				+0.5
3,476,941	-				-1.2
3,476,941	-				-0.1
3,476,941	-				-0.6
3,476,972	-				-0.4
3,477,015	-	kefB	b3350	0.11	-0.1
3,477,021	-				+0.8
3,477,047	-	kefB	b3350	0.12	+1.1
3,477,096	+	kefB	b3350	0.15	-0.3
3,477,096	+	kefB	b3350	0.15	+0.4
3,477,101	+	kefB	b3350	0.15	-0.9
3,477,101	+	kefB	b3350	0.15	-0.0
3,477,109	-	kefB	b3350	0.16	+1.5
3,477,109	-	kefB	b3350	0.16	-1.9
3,477,111	+	kefB	b3350	0.16	+0.2
3,477,111	+	kefB	b3350	0.16	+2.4
3,477,113	-	kefB	b3350	0.16	+1.2
3,477,113	-	kefB	b3350	0.16	-0.1
3,477,129	-				+1.6
3,477,129	-	kefB	b3350	0.17	+1.1
3,477,131	+	kefB	b3350	0.17	+2.7
3,477,131	-	kefB	b3350	0.17	+1.8
3,477,131	-	kefB	b3350	0.17	+0.7
3,477,164	-	kefB	b3350	0.19	-0.2
3,477,164	-	kefB	b3350	0.19	-0.3
3,477,189	+	kefB	b3350	0.20	-0.1
3,477,189	+	kefB	b3350	0.20	-1.5
3,477,197	-	kefB	b3350	0.21	-2.2
3,477,197	-	kefB	b3350	0.21	-0.6
3,477,201	+	kefB	b3350	0.21	+2.1
3,477,218	-	kefB	b3350	0.22	+1.7
3,477,219	-	kefB	b3350	0.22	-0.5
3,477,256	+	kefB	b3350	0.24	-1.4
3,477,256	+	kefB	b3350	0.24	+2.3
3,477,271	-	kefB	b3350	0.25	-1.3
3,477,271	-	kefB	b3350	0.25	-0.3
3,477,313	-	kefB	b3350	0.27	-0.1
3,477,313	-	kefB	b3350	0.27	-0.4
3,477,326	+	kefB	b3350	0.28	-0.4
3,477,337	-	kefB	b3350	0.28	+0.4
3,477,337	-	kefB	b3350	0.28	+2.4
3,477,350	-	kefB	b3350	0.29	+1.7
3,477,388	+	kefB	b3350	0.31	-0.4
3,477,417	-	kefB	b3350	0.33	+2.1
3,477,506	+	kefB	b3350	0.38	-0.8
3,477,515	-	kefB	b3350	0.38	+1.1