Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yghJ and yghK are separated by 484 nucleotides yghK and glcB are separated by 354 nucleotides
b4466: yghJ - predicted inner membrane lipoprotein (NCBI), at 3,112,572 to 3,117,134
yghJ
b2975: yghK - glycolate transporter (NCBI), at 3,117,619 to 3,119,301
yghK
b2976: glcB - malate synthase (NCBI), at 3,119,656 to 3,121,827
glcB
Position (kb)
3117
3118
3119
3120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3116.811 kb on + strand at 3116.815 kb on - strand at 3116.824 kb on + strand at 3116.849 kb on + strand at 3116.929 kb on - strand at 3117.026 kb on - strand at 3117.067 kb on - strand at 3117.072 kb on + strand at 3117.074 kb on + strand at 3117.077 kb on + strand at 3117.077 kb on + strand at 3117.077 kb on - strand at 3117.080 kb on - strand at 3117.080 kb on - strand at 3117.085 kb on + strand at 3117.092 kb on - strand at 3117.132 kb on + strand at 3117.132 kb on + strand at 3117.146 kb on - strand at 3117.211 kb on + strand at 3117.275 kb on + strand at 3117.283 kb on - strand at 3117.408 kb on + strand at 3117.408 kb on + strand at 3117.412 kb on + strand at 3117.430 kb on - strand at 3117.430 kb on - strand at 3117.441 kb on + strand at 3117.586 kb on + strand at 3117.599 kb on - strand at 3117.599 kb on - strand at 3117.611 kb on - strand at 3117.611 kb on - strand at 3117.633 kb on - strand at 3117.640 kb on + strand at 3117.642 kb on + strand at 3117.644 kb on + strand at 3117.645 kb on - strand at 3117.649 kb on + strand at 3117.650 kb on + strand at 3117.650 kb on + strand at 3117.650 kb on + strand at 3117.650 kb on - strand at 3117.650 kb on - strand at 3117.650 kb on - strand at 3117.651 kb on + strand at 3117.652 kb on - strand at 3117.657 kb on - strand at 3117.659 kb on - strand at 3117.662 kb on + strand at 3117.665 kb on - strand at 3117.697 kb on + strand at 3117.702 kb on + strand at 3117.702 kb on + strand at 3117.707 kb on + strand at 3117.707 kb on + strand at 3117.710 kb on - strand at 3117.710 kb on - strand at 3117.752 kb on - strand at 3117.755 kb on - strand at 3117.757 kb on + strand at 3117.757 kb on + strand at 3117.830 kb on + strand, within yghK at 3117.885 kb on + strand, within yghK at 3117.885 kb on + strand, within yghK at 3117.893 kb on - strand, within yghK at 3117.896 kb on - strand, within yghK at 3117.915 kb on - strand, within yghK at 3117.965 kb on - strand, within yghK at 3117.968 kb on - strand, within yghK at 3117.975 kb on + strand, within yghK at 3118.027 kb on + strand, within yghK at 3118.027 kb on + strand, within yghK at 3118.035 kb on - strand, within yghK at 3118.035 kb on - strand, within yghK at 3118.050 kb on + strand, within yghK at 3118.054 kb on + strand, within yghK at 3118.054 kb on + strand, within yghK at 3118.107 kb on + strand, within yghK at 3118.113 kb on + strand, within yghK at 3118.127 kb on - strand, within yghK at 3118.127 kb on - strand, within yghK at 3118.130 kb on + strand, within yghK at 3118.157 kb on - strand, within yghK at 3118.249 kb on - strand, within yghK at 3118.249 kb on - strand, within yghK at 3118.329 kb on + strand, within yghK at 3118.332 kb on + strand, within yghK at 3118.332 kb on + strand, within yghK at 3118.333 kb on + strand, within yghK at 3118.336 kb on - strand, within yghK at 3118.342 kb on - strand, within yghK at 3118.342 kb on - strand, within yghK at 3118.342 kb on - strand, within yghK at 3118.344 kb on + strand, within yghK at 3118.344 kb on + strand, within yghK at 3118.347 kb on - strand, within yghK at 3118.347 kb on - strand, within yghK at 3118.347 kb on - strand, within yghK at 3118.354 kb on + strand, within yghK at 3118.356 kb on + strand, within yghK at 3118.372 kb on - strand, within yghK at 3118.382 kb on + strand, within yghK at 3118.386 kb on + strand, within yghK at 3118.391 kb on + strand, within yghK at 3118.393 kb on + strand, within yghK at 3118.401 kb on - strand, within yghK at 3118.408 kb on - strand, within yghK at 3118.413 kb on + strand, within yghK at 3118.440 kb on - strand, within yghK at 3118.440 kb on - strand, within yghK at 3118.443 kb on + strand, within yghK at 3118.443 kb on + strand, within yghK at 3118.456 kb on - strand, within yghK at 3118.458 kb on + strand, within yghK at 3118.458 kb on + strand, within yghK at 3118.466 kb on - strand, within yghK at 3118.466 kb on - strand, within yghK at 3118.515 kb on - strand, within yghK at 3118.530 kb on - strand, within yghK at 3118.576 kb on - strand, within yghK at 3118.606 kb on + strand, within yghK at 3118.606 kb on + strand, within yghK at 3118.641 kb on - strand, within yghK at 3118.738 kb on - strand, within yghK at 3118.811 kb on - strand, within yghK at 3118.816 kb on - strand, within yghK at 3118.817 kb on + strand, within yghK at 3118.817 kb on + strand, within yghK at 3118.866 kb on + strand, within yghK at 3118.901 kb on - strand, within yghK at 3118.901 kb on - strand, within yghK at 3118.902 kb on - strand, within yghK at 3118.907 kb on - strand, within yghK at 3118.912 kb on + strand, within yghK at 3118.918 kb on - strand, within yghK at 3118.931 kb on - strand, within yghK at 3118.939 kb on + strand, within yghK at 3118.942 kb on + strand, within yghK at 3118.948 kb on + strand, within yghK at 3118.948 kb on + strand, within yghK at 3118.958 kb on - strand, within yghK at 3118.976 kb on - strand, within yghK at 3118.977 kb on + strand, within yghK at 3119.005 kb on - strand, within yghK at 3119.005 kb on - strand, within yghK at 3119.005 kb on - strand, within yghK at 3119.015 kb on - strand, within yghK at 3119.015 kb on - strand, within yghK at 3119.015 kb on - strand, within yghK at 3119.020 kb on + strand, within yghK at 3119.048 kb on + strand, within yghK at 3119.089 kb on + strand, within yghK at 3119.094 kb on - strand, within yghK at 3119.148 kb on + strand at 3119.156 kb on - strand at 3119.160 kb on - strand at 3119.160 kb on - strand at 3119.161 kb on - strand at 3119.174 kb on - strand at 3119.174 kb on - strand at 3119.232 kb on + strand at 3119.242 kb on + strand at 3119.242 kb on + strand at 3119.242 kb on + strand at 3119.245 kb on - strand at 3119.250 kb on - strand at 3119.250 kb on - strand at 3119.250 kb on - strand at 3119.251 kb on + strand at 3119.251 kb on + strand at 3119.251 kb on + strand at 3119.252 kb on + strand at 3119.252 kb on - strand at 3119.254 kb on + strand at 3119.254 kb on + strand at 3119.255 kb on - strand at 3119.256 kb on + strand at 3119.256 kb on + strand at 3119.256 kb on + strand at 3119.257 kb on + strand at 3119.257 kb on + strand at 3119.259 kb on - strand at 3119.259 kb on - strand at 3119.260 kb on - strand at 3119.260 kb on - strand at 3119.264 kb on + strand at 3119.264 kb on - strand at 3119.264 kb on - strand at 3119.264 kb on - strand at 3119.264 kb on - strand at 3119.265 kb on - strand at 3119.268 kb on - strand at 3119.272 kb on - strand at 3119.281 kb on - strand at 3119.281 kb on - strand at 3119.282 kb on - strand at 3119.295 kb on - strand at 3119.314 kb on + strand at 3119.379 kb on + strand at 3119.379 kb on + strand at 3119.390 kb on + strand at 3119.393 kb on - strand at 3119.393 kb on - strand at 3119.398 kb on + strand at 3119.398 kb on + strand at 3119.400 kb on - strand at 3119.400 kb on - strand at 3119.403 kb on + strand at 3119.403 kb on + strand at 3119.411 kb on - strand at 3119.411 kb on - strand at 3119.411 kb on - strand at 3119.412 kb on - strand at 3119.428 kb on - strand at 3119.442 kb on - strand at 3119.442 kb on - strand at 3119.449 kb on - strand at 3119.451 kb on + strand at 3119.451 kb on + strand at 3119.456 kb on - strand at 3119.459 kb on - strand at 3119.460 kb on + strand at 3119.465 kb on + strand at 3119.468 kb on - strand at 3119.475 kb on + strand at 3119.475 kb on + strand at 3119.475 kb on + strand at 3119.482 kb on + strand at 3119.483 kb on + strand at 3119.483 kb on + strand at 3119.483 kb on - strand at 3119.485 kb on + strand at 3119.487 kb on - strand at 3119.489 kb on + strand at 3119.489 kb on + strand at 3119.490 kb on + strand at 3119.490 kb on - strand at 3119.493 kb on - strand at 3119.493 kb on - strand at 3119.495 kb on - strand at 3119.495 kb on - strand at 3119.498 kb on - strand at 3119.499 kb on - strand at 3119.500 kb on - strand at 3119.500 kb on - strand at 3119.526 kb on + strand at 3119.543 kb on + strand at 3119.543 kb on + strand at 3119.603 kb on + strand at 3119.603 kb on + strand at 3119.605 kb on + strand at 3119.605 kb on + strand at 3119.607 kb on + strand at 3119.607 kb on - strand at 3119.613 kb on - strand at 3119.613 kb on - strand at 3119.613 kb on - strand at 3119.615 kb on - strand at 3119.615 kb on - strand at 3119.615 kb on - strand at 3119.616 kb on + strand at 3119.620 kb on - strand at 3119.663 kb on - strand at 3119.695 kb on - strand at 3119.695 kb on - strand at 3119.698 kb on - strand at 3119.704 kb on + strand at 3119.711 kb on - strand at 3119.713 kb on - strand at 3119.721 kb on + strand at 3119.729 kb on - strand at 3119.729 kb on - strand at 3119.762 kb on + strand at 3119.762 kb on - strand at 3119.762 kb on - strand at 3119.795 kb on - strand at 3119.795 kb on - strand at 3119.801 kb on + strand at 3119.873 kb on - strand at 3119.885 kb on - strand, within glcB at 3119.896 kb on - strand, within glcB at 3119.943 kb on - strand, within glcB at 3119.943 kb on - strand, within glcB at 3119.944 kb on - strand, within glcB at 3119.991 kb on + strand, within glcB at 3120.021 kb on - strand, within glcB at 3120.021 kb on - strand, within glcB at 3120.152 kb on - strand, within glcB at 3120.152 kb on - strand, within glcB at 3120.162 kb on - strand, within glcB at 3120.162 kb on - strand, within glcB at 3120.192 kb on + strand, within glcB at 3120.201 kb on + strand, within glcB at 3120.260 kb on + strand, within glcB at 3120.260 kb on + strand, within glcB at 3120.269 kb on - strand, within glcB
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,116,811 + -0.7 3,116,815 - +0.4 3,116,824 + -0.4 3,116,849 + +0.6 3,116,929 - -0.3 3,117,026 - +1.1 3,117,067 - +0.3 3,117,072 + -1.3 3,117,074 + +0.5 3,117,077 + -0.6 3,117,077 + -1.4 3,117,077 - +0.4 3,117,080 - -0.2 3,117,080 - +0.7 3,117,085 + +0.5 3,117,092 - +1.8 3,117,132 + -0.2 3,117,132 + -0.7 3,117,146 - -2.4 3,117,211 + -0.5 3,117,275 + -2.4 3,117,283 - -2.8 3,117,408 + -2.9 3,117,408 + -2.4 3,117,412 + +1.1 3,117,430 - -1.7 3,117,430 - -0.9 3,117,441 + -0.9 3,117,586 + -1.4 3,117,599 - -2.1 3,117,599 - +2.1 3,117,611 - -0.8 3,117,611 - -0.8 3,117,633 - -0.6 3,117,640 + -1.5 3,117,642 + +0.5 3,117,644 + +1.1 3,117,645 - -0.3 3,117,649 + -1.0 3,117,650 + +0.3 3,117,650 + -3.5 3,117,650 + +1.6 3,117,650 - -2.4 3,117,650 - +1.6 3,117,650 - +2.4 3,117,651 + +0.6 3,117,652 - +0.3 3,117,657 - +0.3 3,117,659 - +0.9 3,117,662 + -1.5 3,117,665 - -1.7 3,117,697 + -0.5 3,117,702 + -0.4 3,117,702 + -0.1 3,117,707 + -0.6 3,117,707 + +0.4 3,117,710 - -0.8 3,117,710 - -2.4 3,117,752 - +0.6 3,117,755 - -0.8 3,117,757 + +0.5 3,117,757 + -0.4 3,117,830 + yghK b2975 0.13 +1.6 3,117,885 + yghK b2975 0.16 -0.8 3,117,885 + yghK b2975 0.16 +2.9 3,117,893 - yghK b2975 0.16 +0.4 3,117,896 - yghK b2975 0.16 +1.8 3,117,915 - yghK b2975 0.18 -0.8 3,117,965 - yghK b2975 0.21 -0.2 3,117,968 - yghK b2975 0.21 -1.1 3,117,975 + yghK b2975 0.21 -1.1 3,118,027 + yghK b2975 0.24 -1.1 3,118,027 + yghK b2975 0.24 +0.3 3,118,035 - yghK b2975 0.25 -1.4 3,118,035 - yghK b2975 0.25 +1.9 3,118,050 + yghK b2975 0.26 -0.9 3,118,054 + yghK b2975 0.26 -1.1 3,118,054 + yghK b2975 0.26 +2.5 3,118,107 + yghK b2975 0.29 +1.2 3,118,113 + yghK b2975 0.29 +0.1 3,118,127 - yghK b2975 0.30 +0.6 3,118,127 - yghK b2975 0.30 -0.7 3,118,130 + yghK b2975 0.30 +0.5 3,118,157 - yghK b2975 0.32 +0.4 3,118,249 - yghK b2975 0.37 +0.4 3,118,249 - yghK b2975 0.37 +0.7 3,118,329 + yghK b2975 0.42 +1.0 3,118,332 + yghK b2975 0.42 +2.1 3,118,332 + yghK b2975 0.42 -0.8 3,118,333 + yghK b2975 0.42 +0.2 3,118,336 - yghK b2975 0.43 -0.6 3,118,342 - yghK b2975 0.43 +2.0 3,118,342 - yghK b2975 0.43 -2.3 3,118,342 - yghK b2975 0.43 -0.5 3,118,344 + yghK b2975 0.43 +1.9 3,118,344 + yghK b2975 0.43 +1.0 3,118,347 - yghK b2975 0.43 -0.4 3,118,347 - yghK b2975 0.43 -0.9 3,118,347 - yghK b2975 0.43 +0.3 3,118,354 + yghK b2975 0.44 +1.2 3,118,356 + yghK b2975 0.44 +1.9 3,118,372 - yghK b2975 0.45 -0.8 3,118,382 + yghK b2975 0.45 -2.1 3,118,386 + yghK b2975 0.46 -1.4 3,118,391 + yghK b2975 0.46 -0.1 3,118,393 + yghK b2975 0.46 +1.2 3,118,401 - yghK b2975 0.46 -0.2 3,118,408 - yghK b2975 0.47 +1.2 3,118,413 + yghK b2975 0.47 +2.6 3,118,440 - yghK b2975 0.49 +2.5 3,118,440 - yghK b2975 0.49 -0.5 3,118,443 + yghK b2975 0.49 -0.4 3,118,443 + yghK b2975 0.49 -0.1 3,118,456 - yghK b2975 0.50 +0.1 3,118,458 + yghK b2975 0.50 +1.0 3,118,458 + yghK b2975 0.50 +0.7 3,118,466 - yghK b2975 0.50 +0.7 3,118,466 - yghK b2975 0.50 -0.1 3,118,515 - yghK b2975 0.53 -0.9 3,118,530 - yghK b2975 0.54 +1.5 3,118,576 - yghK b2975 0.57 +0.9 3,118,606 + yghK b2975 0.59 +0.5 3,118,606 + yghK b2975 0.59 +0.6 3,118,641 - yghK b2975 0.61 +1.0 3,118,738 - yghK b2975 0.66 +1.5 3,118,811 - yghK b2975 0.71 +0.7 3,118,816 - yghK b2975 0.71 +1.0 3,118,817 + yghK b2975 0.71 +0.2 3,118,817 + yghK b2975 0.71 +2.2 3,118,866 + yghK b2975 0.74 -0.1 3,118,901 - yghK b2975 0.76 -0.5 3,118,901 - yghK b2975 0.76 +0.0 3,118,902 - yghK b2975 0.76 +3.0 3,118,907 - yghK b2975 0.77 +0.4 3,118,912 + yghK b2975 0.77 +0.4 3,118,918 - yghK b2975 0.77 +1.3 3,118,931 - yghK b2975 0.78 -0.2 3,118,939 + yghK b2975 0.78 -0.4 3,118,942 + yghK b2975 0.79 -0.2 3,118,948 + yghK b2975 0.79 +1.2 3,118,948 + yghK b2975 0.79 +0.8 3,118,958 - yghK b2975 0.80 +0.8 3,118,976 - yghK b2975 0.81 +0.8 3,118,977 + yghK b2975 0.81 +1.2 3,119,005 - yghK b2975 0.82 -1.3 3,119,005 - yghK b2975 0.82 -0.3 3,119,005 - yghK b2975 0.82 -0.4 3,119,015 - yghK b2975 0.83 -2.3 3,119,015 - yghK b2975 0.83 -1.5 3,119,015 - yghK b2975 0.83 +1.2 3,119,020 + yghK b2975 0.83 +0.1 3,119,048 + yghK b2975 0.85 -1.5 3,119,089 + yghK b2975 0.87 +0.3 3,119,094 - yghK b2975 0.88 -0.3 3,119,148 + +0.5 3,119,156 - +1.4 3,119,160 - +0.5 3,119,160 - -1.2 3,119,161 - +0.3 3,119,174 - -0.7 3,119,174 - -0.8 3,119,232 + +0.1 3,119,242 + -0.9 3,119,242 + +1.9 3,119,242 + +0.6 3,119,245 - +1.4 3,119,250 - +1.2 3,119,250 - -1.3 3,119,250 - +0.2 3,119,251 + +1.9 3,119,251 + -1.9 3,119,251 + -1.0 3,119,252 + +1.5 3,119,252 - +2.1 3,119,254 + -0.6 3,119,254 + -0.6 3,119,255 - +1.0 3,119,256 + +0.1 3,119,256 + -2.8 3,119,256 + +1.0 3,119,257 + +1.6 3,119,257 + +0.3 3,119,259 - +0.8 3,119,259 - +1.9 3,119,260 - -1.9 3,119,260 - -1.8 3,119,264 + -1.1 3,119,264 - -1.2 3,119,264 - +0.4 3,119,264 - -1.7 3,119,264 - -1.0 3,119,265 - -1.1 3,119,268 - +1.6 3,119,272 - -0.1 3,119,281 - +0.4 3,119,281 - +0.4 3,119,282 - +0.2 3,119,295 - +0.9 3,119,314 + -0.9 3,119,379 + -0.1 3,119,379 + +1.4 3,119,390 + +0.1 3,119,393 - +1.1 3,119,393 - +1.0 3,119,398 + -2.7 3,119,398 + -1.0 3,119,400 - -0.9 3,119,400 - +0.3 3,119,403 + -0.3 3,119,403 + +0.1 3,119,411 - +0.6 3,119,411 - -0.5 3,119,411 - +0.8 3,119,412 - -0.4 3,119,428 - +1.1 3,119,442 - +1.0 3,119,442 - +1.0 3,119,449 - +0.5 3,119,451 + +0.6 3,119,451 + +0.2 3,119,456 - +2.0 3,119,459 - -0.6 3,119,460 + +2.0 3,119,465 + +0.1 3,119,468 - -0.3 3,119,475 + +1.1 3,119,475 + +1.4 3,119,475 + -0.5 3,119,482 + +1.7 3,119,483 + +3.2 3,119,483 + +1.2 3,119,483 - -2.3 3,119,485 + +1.0 3,119,487 - -0.1 3,119,489 + +1.1 3,119,489 + +0.6 3,119,490 + +0.9 3,119,490 - -0.3 3,119,493 - +0.2 3,119,493 - +3.0 3,119,495 - -3.4 3,119,495 - +2.4 3,119,498 - +0.1 3,119,499 - +1.1 3,119,500 - +0.5 3,119,500 - +0.8 3,119,526 + -0.7 3,119,543 + -0.3 3,119,543 + +0.3 3,119,603 + +2.0 3,119,603 + +1.5 3,119,605 + +0.2 3,119,605 + -1.8 3,119,607 + -0.5 3,119,607 - -0.4 3,119,613 - -1.3 3,119,613 - +1.0 3,119,613 - -1.1 3,119,615 - +0.5 3,119,615 - +0.3 3,119,615 - +0.0 3,119,616 + -0.4 3,119,620 - -1.5 3,119,663 - +4.0 3,119,695 - -1.2 3,119,695 - -0.3 3,119,698 - -0.9 3,119,704 + -1.2 3,119,711 - +2.1 3,119,713 - +0.8 3,119,721 + -0.8 3,119,729 - +2.3 3,119,729 - +0.2 3,119,762 + +0.7 3,119,762 - +0.9 3,119,762 - -0.1 3,119,795 - +3.0 3,119,795 - +2.1 3,119,801 + -3.1 3,119,873 - +1.5 3,119,885 - glcB b2976 0.11 -2.7 3,119,896 - glcB b2976 0.11 +1.1 3,119,943 - glcB b2976 0.13 -0.8 3,119,943 - glcB b2976 0.13 +1.1 3,119,944 - glcB b2976 0.13 -1.7 3,119,991 + glcB b2976 0.15 -0.4 3,120,021 - glcB b2976 0.17 -1.3 3,120,021 - glcB b2976 0.17 +0.6 3,120,152 - glcB b2976 0.23 +1.1 3,120,152 - glcB b2976 0.23 -0.8 3,120,162 - glcB b2976 0.23 -1.5 3,120,162 - glcB b2976 0.23 -1.8 3,120,192 + glcB b2976 0.25 +0.9 3,120,201 + glcB b2976 0.25 +0.9 3,120,260 + glcB b2976 0.28 +1.7 3,120,260 + glcB b2976 0.28 +1.0 3,120,269 - glcB b2976 0.28 +0.9
Or see this region's nucleotide sequence